Patents Examined by Ruth Davis
  • Methods for quantifying particulates in cell culture
    Patent number: 10564087
    Abstract: Provided are methods for quantifying and/or detecting sub-visible particulates in cell cultures. Specifically, the methods comprise a step of breaking down, e.g., lysing, cells in a cell culture. The methods can further comprising filtering the cell culture through a filter. Further provided are methods of quantifying sub-visible particulates that do not pass through the filter using a microscope.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: February 18, 2020
    Assignee: Celularity, Inc.
    Inventors: Lauren M. Depalma, Daniel Dewitt, Li Ren
  • Cell bandage
    Patent number: 10549012
    Abstract: The invention provides a method for delivering cells across the surface of a tissue, the method comprising distributing the cells on and/or within a biomaterial to form a cell bandage and applying the cell bandage to the surface, wherein, after application of the cell bandage to the surface of the tissue, the cells are released from the cell bandage. Further provided is a method for bonding two or more tissues, the method comprising providing a cell bandage in intimate contact with the surfaces to be joined, wherein the cell bandage comprises a biomaterial, said biomaterial having cells distributed on and/or within it.
    Type: Grant
    Filed: January 9, 2017
    Date of Patent: February 4, 2020
    Assignee: THE UNIVERSITY OF BRISTOL
    Inventors: Anthony P. Hollander, Wa'el Z. Kafienah, Ehsanollah Esfandiari, John F. Tarlton
  • Use of for promoting recovery of the intestinal microbiota diversity after dysbiosis
    Patent number: 10548928
    Abstract: The present invention provides the use of Lactobacillus paracasei, for maintaining or increasing the intestinal microbiota diversity in a subject having dysbiosis.
    Type: Grant
    Filed: April 15, 2015
    Date of Patent: February 4, 2020
    Assignees: COMPAGNIE GERVAIS DANONE, INSTITUT NATIONAL DE LA RECHERCHE AGRNOMIQUE
    Inventors: Gianfranco Grompone, Muriel Derrien, Johan Van Hylckama Vlieg, Pascale Serror, Lionel Rigottier-Gois, Laureen Crouzet, Claire Cherbuy
  • Process of cultivating microalgae and a joint method of same with a denitration process
    Patent number: 10533154
    Abstract: The present invention provides a joint method of cultivating microalgae combined with denitrating an industrial waste gas and a system useful for the same. The joint method comprises the steps of: (1) a step of cultivating microalgae; (2) a separation step of separating a microalgae suspension obtained from step (1) into a wet microalgae (microalgae biomass) and a residual cultivation solution; (3) a NOx absorbing/immobilizing step of denitrating an industrial waste gas with the residual cultivation solution obtained from step (2); wherein the nutrient stream absorbed with NOx obtained from step (3) is used to provide nitrogen source to the microalgae cultivation of step (1). During the microalgae cultivation, EM bacteria is added into the microalgae suspension. The microalgae is preferably Chlorella sp., Scenedesmus sp., Monoraphidium sp. or Spirulina sp.
    Type: Grant
    Filed: March 17, 2015
    Date of Patent: January 14, 2020
    Assignees: CHINA PETROLEUM & CHEMICAL CORPORATION, RESEARCH INSTITUTE OF PETROLEUM PROCESSING, SINOPEC
    Inventors: Junfeng Rong, Xuhua Zhou, Lin Cheng, Junying Zhu, Xugeng Huang, Baoning Zong
  • Methods and devices for preparation of enriched biological fluids
    Patent number: 10512659
    Abstract: The present disclosure relates to methods and apparatus for producing platelet rich plasma, bone marrow mononuclear cells, stromal vascular fraction from adipose tissue, and other concentrated or enriched biological fluids.
    Type: Grant
    Filed: July 2, 2016
    Date of Patent: December 24, 2019
    Assignee: Arthrex, Inc.
    Inventors: Melissa D. Tucker, Christopher Bare, Abigail Nabors, Robert Harrison
  • Compositions and methods for treating bone, joints and cartilage
    Patent number: 10500233
    Abstract: Briefly stated, the present invention provides compositions and methods for treating or preventing joint injuries utilizing hair follicle derived Non-Bulbar Dermal Sheath (“NDBS”) cells. Within one aspect of the invention methods are provided for isolating NBDS cells, comprising the steps of: (a) preparing vital hair; (b) cleaving the hair prepared in step (a) to remove the hair follicle bulb; (c) isolating Non-Bulbar Dermal Sheath tissue; and (d) cultivating the isolated Non-Bulbar Dermal sheath tissue to produce NBDS cells. Within one embodiment of the invention the vital hair is obtained by full skin biopsy from the occipital scalp of a subject.
    Type: Grant
    Filed: February 12, 2015
    Date of Patent: December 10, 2019
    Assignee: RepliCel Life Sciences Inc.
    Inventors: Rolf Hoffmann, Kevin John McElwee
  • Method and system for imaging and analysis of a biological specimen
    Patent number: 10495554
    Abstract: The present disclosure provides methods of preparing a biological specimen for imaging analysis, comprising fixing and clearing the biological specimen and subsequently analyzing the cleared biological specimen using microscopy. Also included are methods of quantifying cells, for example, active populations of cells in response to a stimulant. The present disclosure also provides devices for practicing the described methods. A flow-assisted clearing device provides rapid clearing of hydrogel-embedded biological specimens without the need of specialized equipment such as electrophoresis or perfusion devices.
    Type: Grant
    Filed: May 25, 2017
    Date of Patent: December 3, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Karl A. Deisseroth, William E. Allen, Brian Hsueh, Li Ye
  • System, apparatus and method for biomolecules production
    Patent number: 10494421
    Abstract: An automated method for the production of cells and/or biomolecules such as protein or peptides includes culturing cells in at least one high cell density bioreactor, thereby fluidly connecting said bioreactor with a culture medium supply and a gas or gaseous mixture; fluidly connecting said bioreactor with a downstream unit; and growing cells to a density at least 50 million cells per ml. The total volume of the bioreactor is at least 10 liters. A system suitable for implementation of the automated method above is a small-scale and cupboard-sized system, which can be placed in a portable clean room.
    Type: Grant
    Filed: February 9, 2015
    Date of Patent: December 3, 2019
    Assignee: UNIVERCELLS NV
    Inventor: José Castillo
  • In vitro pre-conditioned bone marrow-derived mesenchymal stem cells and uses thereof
    Patent number: 10478458
    Abstract: Disclosed is a composition including: an isolated in vitro pre-conditioned population of adult bone marrow derived mesenchymal stem cells (BMSCs), wherein the BMSCs express neuronal markers, and wherein the neuronal markers are PGP9.5, NSE, Tuj1, HuC/D and neuronal nitric oxide synthase (nNOS). Methods of preparing the BMSCs are also provided. In addition, the present disclosure is directed to a method of treating an enteric nervous system-related disorder including: administering to a subject in need thereof a pharmaceutical composition including the in vitro pre-conditioned BMSC population and a pharmaceutically acceptable carrier.
    Type: Grant
    Filed: August 3, 2016
    Date of Patent: November 19, 2019
    Assignees: THE JOHNS HOPKINS UNIVERSITY, UNION HOSPITAL, TONGJI MEDICAL COLLEGE, HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY
    Inventors: Xuhang Li, Xiaohua Hou, Rong Lin
  • HAR-NDS-derived stem cells, method for separating same, and use thereof
    Patent number: 10471097
    Abstract: The present invention relates to hyaluronic acid-rich node and duct system (HAR-NDS)-derived stem cells, a method for separating the same, and a use thereof and, more specifically, to node and ductal stem cells (NDSCs), which are adult stem cells having an ability to differentiate into HAR-NDS-derived neural cells, and hematopoietic stem cells having an ability to differentiate into blood cells. The present invention is capable of separating, from HAR-NDS, adult stem cells NDSCs and hematopoietic stem cells, which are not easy to obtain from bone marrow, peripheral blood and umbilical cord blood (cord blood), as an alternative source, and thus can be usefully used for treatment of brain diseases, neurological diseases, chronic infectious diseases, cancers, autoimmune diseases, organ regeneration treatments and various intractable diseases.
    Type: Grant
    Filed: April 28, 2015
    Date of Patent: November 12, 2019
    Assignee: Eutilex Co., Ltd.
    Inventor: Byoung Se Kwon
  • Methods and materials for assessing and treating arthritis
    Patent number: 10465224
    Abstract: This document provides methods and materials involved in assessing mammals (e.g., humans) for arthritis. For example, methods and materials for assessing a mammal's gut microbial diversity to identify the mammal as having arthritis (e.g., rheumatoid arthritis) are provided. This document also provides methods and materials involved in treating arthritis.
    Type: Grant
    Filed: February 3, 2016
    Date of Patent: November 5, 2019
    Assignee: Mayo Foundation for Medical Education and Research
    Inventor: Veena Taneja
  • Blends of strains and enzymes
    Patent number: 10463701
    Abstract: Compositions and methods are disclosed for providing beneficial effects to animals, including but not limited to increasing performance of the animal. In one embodiment, the animal is poultry. In another embodiment, the disclosure relates to a composition comprising one or more direct-fed microbials and one or more exogenous feed enzymes.
    Type: Grant
    Filed: December 30, 2014
    Date of Patent: November 5, 2019
    Assignee: DUPONT NUTRITION BIOSCIENCE APS
    Inventors: Janet Remus, Randolph Chick, Nuntawadee Sriperm, Tammy Baltzley, Milan Hruby
  • Selective near-infrared optical imaging of necrotic cells and simultaneous cell fixing and counter staining with metallacrown complexes
    Patent number: 10458991
    Abstract: In an example of a method for simultaneously fixing and staining cells, the cells are initially incubated in a solution including a Ln(III)Zn16(HA ligand)16 metallacrown complex, wherein the HA ligand is a hydroximate ligand. The incubating cells are exposed to ultraviolet (UV) light. The cells are allowed to continue to incubate in the solution after UV light exposure.
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: October 29, 2019
    Assignees: The Regents of the University of Michigan, Centre National de la Recherche Scientifique
    Inventors: Ivana Martinic, Tu Ngoc Nguyen, Svetlana V. Eliseeva, Vincent L. Pecoraro, Stéphane Petoud
  • Conditioned medium of liver progenitor cells
    Patent number: 10456425
    Abstract: The invention is in the field of regenerative medicine. It has been found that the conditioned medium of non-oval pluripotent liver progenitor cells exerts a tissue regenerating effect. A preparation of the cell free conditioned medium is therefore useful in the treatment of injury and organ failure, preferably liver and/or injury or failure.
    Type: Grant
    Filed: June 11, 2009
    Date of Patent: October 29, 2019
    Assignee: FRESENIUS MEDICAL CARE DEUTSCHLAND GMBH
    Inventors: Maria Beatriz Herrera Sanchez, Valentina Fonsato, Ciro Tetta, Giovanni Camussi
  • Composition and solution with controlled calcium ion level, and related method and use for reperfusion
    Patent number: 10433539
    Abstract: A solution comprises a preservation mixture comprising a calcium ion source; and a buffer for maintaining a pH of the solution. The molar concentration of calcium ion (Ca2+) in the solution is from 0.18 to 0.26 mmol/L, and the pH is lower than 7.4 and higher than 6.6. A composition for preparing the solution may comprise adenosine, lidocaine, and a calcium source, wherein the molar ratio of adenosine:calcium is from 0.3:0.26 to 0.45:0.18, and the molar ratio of lidocaine:calcium is from 0.04:0.26 to 0.09:0.18. A donor heart may be reperfused with the solution. The solution may be used for reperfusion of a donor heart, such as at a temperature from about 25 to about 37° C. The donor may be a donor after circulatory death.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: October 8, 2019
    Assignee: TEVOSOL, INC.
    Inventors: Christopher White, Darren Freed, Larry Hryshko
  • Banana fermentation product and manufacturing method and use of the same
    Patent number: 10426811
    Abstract: The present invention provides a banana fermentation product, which is obtained by subjecting a banana pulp juice to a pre-fermentation and then a post-fermentation, wherein the pre-fermentation is conducted in the presence of Streptococcus thermophiles and yeast and the post-fermentation is conducted in the presence of Acetobacter. The banana fermentation product of the present invention is effective in anti-oxidation, increasing the amount of probiotics in the intestinal tract, reducing the amount of bad bacteria in the intestinal tract, and relieving constipation symptoms. The banana fermentation product of the present invention can further be used for providing an edible composition or a pharmaceutical composition.
    Type: Grant
    Filed: August 24, 2017
    Date of Patent: October 1, 2019
    Assignee: TCI CO., LTD.
    Inventors: Yung-Hsiang Lin, Po-Chun Hsu, Cheng-Yu Ho
  • Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
    Patent number: 10420803
    Abstract: Cell populations of intestinal midgut endoderm cells and methods of generating the cells expressing markers characteristic of intestinal endoderm lineage are disclosed. Methods of treating conditions such as diabetes are also disclosed.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: September 24, 2019
    Assignee: Janssen Biotech, Inc.
    Inventors: Sebastian Rieck, Alireza Rezania
  • Transglutaminase treated products
    Patent number: 10413634
    Abstract: The present application relates to use of transglutaminases to treat various products, including medical devices such as tissue grafts, tissue matrices or other tissue-derived materials, and synthetics. The transglutaminases can be applied to the medical devices to provide advantages such as adhesion resistance or abrasion resistance.
    Type: Grant
    Filed: January 29, 2018
    Date of Patent: September 17, 2019
    Assignee: LifeCell Corporation
    Inventors: Yi Chen, Sean Collins, Li Ting Huang, Eric Stec, Hui Xu
  • Stem cell, bone, tissue and nerve scaffolding from discrete carbon nanotubes
    Patent number: 10414655
    Abstract: Stem cell, bone and nerve scaffolding comprising discrete carbon nanotubes is disclosed. The discrete carbon nanotubes may be have targeted, or selective oxidation levels and/or content on the interior and exterior of the tube walls. The described scaffolding may be used to guide, target and protect stem cells upon injection into the body.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: September 17, 2019
    Assignee: Molecular Rebar Design, LLC
    Inventors: Clive P. Bosnyak, Kurt W. Swogger, Nancy Henderson, Paul Everill
  • Method for packaging fungal spores in a modified atmosphere with a view to increasing the shelf life of the fungi
    Patent number: 10407661
    Abstract: The present invention relates to a packaging method for increasing the shelf life of fungal spores and to the packaging that contains such spores. The packaging method comprises the steps of pre-drying the spores to a water activity range viable for the fungus of interest, followed by packing of the spores into gas- and water-vapor-impermeable packaging by means of the use of sachets, thereby providing an atmosphere of low relative humidity and low oxygen content, followed by keeping of the spores in the packaging for a certain period of time at the temperature suitable for the spores prior to the exposure thereof to high temperatures.
    Type: Grant
    Filed: August 1, 2011
    Date of Patent: September 10, 2019
    Assignee: EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA—EMBRAPA
    Inventor: Marcos Rodrigues De Faria