Abstract: The present disclosure relates to compositions and methods for marking/tagging objects for identification. In particular, tagging objects with a nucleic acid taggant (genetic tag based merchandise authentication).

Abstract: The invention is a novel method of generating a library of circular single stranded nucleic acid molecules by utilizing circular capture molecules. The method is not limited by size of target nucleic acid molecules and can potentially accommodate very long molecules. The method finds application in nucleic acid sequencing, e.g., nanopore sequencing where unlimited-length templates can be read.

Abstract: Compositions, methods and kits for detecting Chikungunya virus (CHIKV) nucleic acids are for detecting very low levels of the viral nucleic acids using nucleic acid amplification. The kit includes a first primer up to 100 bases long and includes a target-complementary 3? terminal sequence of 15-48 contiguous bases. The first primer optionally may include a first primer 5? sequence (i.e., an upstream sequence) that is not complementary to CHIKV nucleic acids.

Abstract: Methods of selectively sequencing amplicons in a biological sample are provided.

Abstract: Described herein are methods, compositions and kits directed to amplification of nucleic acids suitable for both next generation sequencing (NGS) and a second round of sequencing as validation, such as Sanger sequencing.

Abstract: The invention provides an antibody-based bioanalytical method, specifically a hybridization Chain Reaction-based Method for Amplifying Immunosignals named immunosignal HCR (isHCR), which combines antibody-antigen interactions with hybridization Chain Reaction (HCR) technology for amplifying immunosignals. The invention also provides a kit for performing the above isHCR.

Abstract: Systems and methods for operating an aquatic robot. The methods comprise: autonomously propelling the aquatic robot through a body of water to a location where a water sample is to be obtained; and performing operations by the aquatic robot to autonomously collect the water sample, cause the water sample to flow through a filter that retains eDNA, lyses and releases the eDNA to a create a lysate, process the lysate to obtain a product for eDNA sequencing, generate eDNA sequencing data using the product, and communicate the eDNA sequencing data to a remote external device.

Abstract: Provided is a PCR tube including: an outer tube configured to provide a first space having a blocked lower portion; and an inner tube inserted to the first space. Here, the inner tube includes: an inner tube body configured to provide a second space; and a spiral structure protruding from a side surface of the inner tube body to the outside of the inner tube body, and a micro-channel is defined by the outer tube, the inner tube body, and the spiral structure in a state in which the inner tube is inserted into the first space.

Abstract: The disclosure provides methods for estimating tumor purity from tumor samples without use of matched-normal controls. A set of genomic regions are identified based on a nucleic acid sequence data that is aligned to a reference genome. Each genomic region of the set of genomic regions includes one or more nucleotide-sequence variants relative to a corresponding genomic region of the reference genome. A B-allele frequency distribution for the biological sample is determined based on a B-allele frequency determined for each genomic region of the set of genomic regions. The B-allele frequency distribution is processed using a trained machine-learning model to estimate a metric identifying tumor purity in the biological sample.

Abstract: Provided herein are methods of sequencing comprising click chemistry bioconjugation. In some embodiments, target polynucleotide sequences on the same or different molecules are contacted with and hybridize to probes comprising click functional groups. Probes (e.g., reading probes) hybridizing to adaptor sequences adjacent to different sequences of interest (e.g., barcodes to be sequenced) can be hybridized simultaneously in large pools. In some embodiments, the provided methods achieve multiplexing without requiring separate hybridization of probes (e.g., reading probes) for each sequencing-by-ligation cycle, thereby reducing total hybridization time which is typically a most time-consuming step in in situ technologies. In some aspects, the hybridized probes (e.g., reading probes) are clicked onto detectable probes to analyze a sequence of a target polynucleotide in a sequencing-by-ligation fashion.

Abstract: A method for screening Lactobacillus casei fermentation agent is provided. The method is specifically implemented by screening a Lactobacillus casei strain with high-expression of a lac cluster gene and no enzymatic activity of ?-fructosidase. The Lactobacillus casei strain that rapidly metabolizes lactose is screened through high expression of the lac cluster gene, such that the problem of large differences in acid production in fermented milk beverage products is avoided.

Abstract: The present disclosure provides methods for molecular neighborhood detection of molecules, such as by iterative proximity ligation or split-and-pool methods for obtaining positional information.

Abstract: The present disclosure relates to a method of analyzing a biofluid of a subject for the presence of bacterial extracellular vesicles (EV), the method comprising the steps of a) extracting bacterial EV from the biofluid, b) analyzing the bacterial EV-extracted molecular patterns for the presence of a disease marker, wherein the disease marker is a disease-specific molecular pattern of the subject; and the subject comprising patients diagnosed with HIV, IBD or cancer or the subject receiving a treatment.

Abstract: The present invention relates to catalytic biomolecule characterization and microfluidics. It is used for identification of nucleic acids encoding active catalytic molecules in the plurality of nucleic acids and for gathering information about catalytic biomolecule activity. It can also be used for exploring different properties of regulating sequences that modulate expression of catalytic biomolecules by recording that information into the DNA sequence of the same catalytic biomolecule using microfluidic techniques.

Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting neoplasms such as cholangiocarcinoma.

Abstract: Provided herein, in some embodiments, are methods and compositions for the production of long single-stranded DNA.

Abstract: Novel means that enables detection of the monocytogenes bacterium alone distinctly from other bacteria belonging to the genus Listeria with sufficiently high accuracy is disclosed. The present inventors intensively analyzed the genome of the monocytogenes bacterium to identify two genes (the lmo0084 gene and the lmo2736 gene) as target regions with which the monocytogenes bacterium can be specifically detected distinctly from other bacteria belonging to the genus Listeria utilizing a nucleic acid amplification method. By a further intensive study of the base sequences of these two genes, primer setting regions for highly accurate, specific detection of the monocytogenes bacterium alone were identified, and preferred particular examples of PCR primer sets, LAMP primer sets, and real-time PCR primer-probe sets were established.

Abstract: Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.

Abstract: Described herein, among other things, is a method of sequencing nucleic acids of interest (NAOIs). In some embodiments the method may comprise: providing a sample comprising NAOIs; attaching oligonucleotides to the NAOIs to provide labelled NAOIs, wherein the oligonucleotides comprise a PCR cycle counter generator sequence comprising at least one universal nucleotide base; amplifying the labelled NAOIs using PCR to provide an amplified library of NAOIs each containing a PCR cycle counter sequence; sequencing the amplified library of NAOIs to provide a set of sequence reads, wherein each sequence read comprises a NAOI-originating component and a PCR cycle counter component; and distinguishing true variants in NAOI sequences from false variants in NAOI sequences.

Abstract: Novel means that enables detection of the monocytogenes bacterium alone distinctly from other bacteria belonging to the genus Listeria with sufficiently high accuracy is disclosed. The present inventors intensively analyzed the genome of the monocytogenes bacterium to identify two genes (the lmo0084 gene and the lmo2736 gene) as target regions with which the monocytogenes bacterium can be specifically detected distinctly from other bacteria belonging to the genus Listeria utilizing a nucleic acid amplification method. By a further intensive study of the base sequences of these two genes, primer setting regions for highly accurate, specific detection of the monocytogenes bacterium alone were identified, and preferred particular examples of PCR primer sets, LAMP primer sets, and real-time PCR primer-probe sets were established.