Patents Examined by Samuel Woolwine
  • Luminophore-labeled molecules coupled with particles for microarray-based assays
    Patent number: 9310375
    Abstract: A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. This technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way.
    Type: Grant
    Filed: October 27, 2010
    Date of Patent: April 12, 2016
    Assignees: CapitalBio Corporation, Tsinghua University
    Inventors: Huafang Gao, Guangxin Xiang, Di Jiang, Wanli Xing, Jing Cheng
  • Use of perturbants to facilitate incorporation and recovery of taggants from polymerized coatings
    Patent number: 9297032
    Abstract: The invention provides methods for increasing the recoverability of taggants from an object. The methods include the steps of incorporating a taggant into a solution; mixing the solution including the taggant with a perturbant to form a first perturbant taggant solution; mixing the first perturbant taggant solution with a polymer to form a second perturbant taggant polymer solution; and applying the second perturbant taggant polymer solution to at least a portion of the object to form a taggant-coated object. Methods for authentication of a taggant marked object are also provided.
    Type: Grant
    Filed: October 10, 2012
    Date of Patent: March 29, 2016
    Assignee: APDN (B.V.I.) Inc.
    Inventors: Lawrence Jung, MingHwa Benjamin Liang, James A. Hayward
  • Method for quantifying human DNA
    Patent number: 9290815
    Abstract: The invention provides for a method for quantifying one or more nucleic acids of a genome in a sample comprising the steps of, (a) amplifying a first nucleic acid to be quantified, (b) determining the amount of said first nucleic acid by comparison of the amount of amplification product from said first nucleic acid with at least one amplification product from a second template nucleic acid, (c) wherein said second template nucleic acid was generated using whole genome amplification and wherein the starting concentration of the second template nucleic acid is known.
    Type: Grant
    Filed: February 21, 2012
    Date of Patent: March 22, 2016
    Assignee: Qiagen GMBH
    Inventors: Francesca Di Pasquale, Holger Engel
  • System and method for detecting biological materials
    Patent number: 9290799
    Abstract: A method of identifying a target biological material by using captures and probes. Each capture is specific for a kind of target. Each probe is designed to distinguish the type of the target. The captures and probes are each labeled with one or more detectable labels.
    Type: Grant
    Filed: August 20, 2013
    Date of Patent: March 22, 2016
    Assignee: Industrial Technology Research Institute
    Inventors: Yi-Chang Chen, Jane SC Tsai
  • Systems and methods for real-time PCR
    Patent number: 9283563
    Abstract: In one aspect, the present invention provides a systems and methods for the real-time amplification and analysis of a sample of DNA.
    Type: Grant
    Filed: October 28, 2008
    Date of Patent: March 15, 2016
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Gregory H. Owen, Gregory A. Dale, Kenton C. Hasson, Shulin Zeng, Dwayne W. Warfield, Sarah Warfield
  • Multiplex amplification and detection
    Patent number: 9284607
    Abstract: The invention relates to the field of multiplex amplification. In particular, the invention relates to methods for assaying a sample for one or more nucleic acid targets in a single reaction based on the distinct melting temperatures or melting profiles of primers and/or probes. The invention also provides probes and kits for use in such methods.
    Type: Grant
    Filed: October 25, 2011
    Date of Patent: March 15, 2016
    Assignee: OXITEC LTD
    Inventor: Guoliang Fu
  • EMX2 in cancer diagnosis and prognosis
    Patent number: 9279157
    Abstract: The present invention provides methods for determining a prognosis of disease free or overall survival in a patient suffering from cancer. The methods generally involve determining a normalized expression level of an EMX2 gene product, which correlates with prognosis and likelihood of survival.
    Type: Grant
    Filed: February 5, 2010
    Date of Patent: March 8, 2016
    Assignee: The Regents of the University of California
    Inventors: Biao He, David M. Jablons
  • Rare cell analysis using sample splitting and DNA tags
    Patent number: 9273355
    Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: March 1, 2016
    Assignees: The General Hospital Corporation, GPB Scientific, LLC, Verinata Health, Inc.
    Inventors: Daniel Shoemaker, Ravi Kapur, Mehmet Toner, Roland B. Stoughton, Ronald W. Davis
  • Methods and compositions for processes of rapid selection and production of nucleic acid aptamers
    Patent number: 9273345
    Abstract: Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
    Type: Grant
    Filed: December 27, 2007
    Date of Patent: March 1, 2016
    Assignee: CONCEPTUAL MINDWORKS, INC.
    Inventors: Johnathan L. Kiel, Eric A. Holwitt, Michael (Maomian) Fan, Shelly D. Roper
  • Detection, identification and differentiation of eubacterial taxa using a hybridization assay
    Patent number: 9273361
    Abstract: The present invention relates to a method for the specific detection and/or identification of Enterococcus species, in particular Enterococcus faecalis and/or Enterococcus faecium, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Enterococcus species, in particular of Enterococcus faecalis and/or Enterococcus faecium, in a biological sample. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Enterococcus species in a sample.
    Type: Grant
    Filed: January 23, 2013
    Date of Patent: March 1, 2016
    Assignees: FUJIREBIO EUROPE N.V., ROCHE DIAGNOSTICS GMBH
    Inventors: Hilde De Henau, Joachim Van Crombruggen, Geert Jannes, Gerd Haberhausen, Thomas Emrich
  • Detection of RNA-interacting regions in DNA
    Patent number: 9273347
    Abstract: The present invention provides methods and kits for detecting RNA-interacting regions in genomic DNA.
    Type: Grant
    Filed: September 9, 2011
    Date of Patent: March 1, 2016
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Steven Okino
  • Systems and methods for genetic and biological analysis
    Patent number: 9274077
    Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: March 1, 2016
    Assignee: GENAPSYS, INC.
    Inventors: Hesaam Esfandyarpour, Kosar Baghbani Parizi, Mark F. Oldham, Eric S. Nordman, Richard T. Reel, Susanne Baumhueter, Cheryl Heiner, Frank Lee
  • Combined thermal devices for thermal cycling
    Patent number: 9272282
    Abstract: The invention relates to systems and methods including a combination of thermal generating device technologies to achieve more efficiency and accuracy in PCR temperature cycling of nucleic samples undergoing amplification.
    Type: Grant
    Filed: August 13, 2013
    Date of Patent: March 1, 2016
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Gregory A. Dale, Shulin Zeng, Kenton C. Hasson
  • Method for making biochips
    Patent number: 9266726
    Abstract: A method for immobilizing biological polymers such as DNA or proteins, on a solid support, by ionocovalent bond, for making biochips, and the resulting chips obtained by the method. Also, a kit for the preparation of the chips.
    Type: Grant
    Filed: July 15, 2010
    Date of Patent: February 23, 2016
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCENTIFIQUE (C.N.R.S), UNIVERSITE DE NANTES, UNIVERSITY OF FLORIDA
    Inventors: Charles Tellier, Muriel Pipelier, Didier Dubreuil, Bruno Bujoli, Daniel Talham
  • Detection and differentiation of demodex mites
    Patent number: 9267178
    Abstract: Methods of detecting D. gatoi or D. cati, are disclosed. A sample suspected of containing a nucleic acid of D. gatoi or D. cati is screened for the presence or absence of that nucleic acid. The presence of the D. gatoi or D. cati nucleic acid indicates the presence of D. gatoi or D. cati. Determining whether the D. gatoi or D. cati nucleic acid is present in the sample can be accomplished by detecting hybridization between a D. gatoi or D. cati probe. Probes and primers for the detection of D. gatoi or D. cati are also disclosed. Disclosed are isolated nucleic acids that encode a D. gatoi or D. cati rRNA gene sequence. Kits and arrays that contain the disclosed probes and/or primers also are disclosed.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: February 23, 2016
    Assignee: UNIVERSITY OF TENNESSEE RESEARCH FOUNDATION
    Inventors: Stephen A. Kania, Linda A. Frank
  • Mobile rapid test system for nucleic acid analysis
    Patent number: 9260750
    Abstract: A mobile rapid test system for nucleic acid analysis. A method comprising the steps of amplification of the nucleic acids by means of rapid-PCR technology, conversion of a double-stranded amplification product into a single-stranded DNA fragment, hybridization with a labeled probe and detection of the nucleic acids on a lateral-flow test strip. A device comprising a reaction cavity which preferably consists of a thin film, inlet and outlet openings for the reaction cavity, one or more heatable sample blocks which are connected to miniaturized cooling bodies and a window for reading off the result. The lateral-flow test strip is a component of the mobile rapid test system. Operation of the instrument system requires no external power source, but only batteries or a rechargeable battery.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: February 16, 2016
    Assignee: AJ INNUSCREEN, GmbH
    Inventors: Timo Hillebrand, Claus Knippschild, Benjamin Jaschinsky, Elmara Graser
  • Method of detecting and assaying telomerase activity of telomerase bound by anti-telomerase antibodies
    Patent number: 9249452
    Abstract: The invention is directed to methods for determining the level of telomerase reverse transcriptase activity in mammalian cells. The method comprises binding the telomerase reverse transcriptase obtained from the mammalian cells to a solid support by contacting the telomerase with an anti-telomerase antibody bound to a solid support and then measuring the level of activity of the bound telomerase in a reaction where the telomerase can extend a first primer to produce an extension product and qualitatively or quantitatively detecting the extension product.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: February 2, 2016
    Inventor: Zhu Zhen Pirot
  • Methods for obtaining a sequence
    Patent number: 9249460
    Abstract: The invention generally relates to methods for obtaining a sequence, such as a consensus sequence or a haplotype sequence. In certain embodiments, methods of the invention involve determining an amount of amplifiable nucleic acid present in a sample, partitioning the nucleic acid based upon results of the determining step such that each partitioned portion includes, on average, a subset of unique sequences, sequencing the nucleic acid to obtain sequence reads, and assembling a consensus sequence from the reads.
    Type: Grant
    Filed: September 10, 2012
    Date of Patent: February 2, 2016
    Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
    Inventors: Dmitry Pushkarev, Stephen R. Quake, Ayelet Voskoboynik, Michael Kertesz
  • System and method for authentication and tamper detection using nucleic acid taggants
    Patent number: 9243283
    Abstract: Methods for authenticating and/or detecting tampering of an item of interest using a nucleic acid tag. A nucleic acid tag comprising a nucleotide-support platform attached to a nucleic acid molecule is created or obtained and then sealed within or on the item of interest. The surface of the item of interest is sampled for the presence of the seeded tag after the item of interest has been moved from one location to another or has been stored for a period of time, during which tampering can occur and/or authentication may necessary. The presence of the tag can indicate that tampering has occurred, or that the item of interest is authentic.
    Type: Grant
    Filed: November 19, 2012
    Date of Patent: January 26, 2016
    Assignee: SRC, Inc.
    Inventors: Mary F. Swartz, Garrett D. Liddil, Adam J. Lowe
  • Polony sequencing methods
    Patent number: 9243290
    Abstract: We describe ultra-high throughput polony genome sequencing that can permit, for example, generating raw data to re-sequencing the human genome in about one week (including library prep and sequencing) at a reasonable cost. The methods described herein include one or more of the following: (1) increasing polony sequencing read length, (2) improving library construction and emulsions protocols, (3) increasing bead density and/or moving to alternative clonal amplication strategies (other than emulsion PCR or ePCR), (4) extending software capabilities to allow SNP calls from our new sequencing raw data, (5) Dual Primer Emulsion PCR, and (6) diagnostic method exploiting one or more of the foregoing.
    Type: Grant
    Filed: October 8, 2010
    Date of Patent: January 26, 2016
    Assignee: STC.UNM
    Inventor: Jeremy Edwards