Patents Examined by Satyendra Singh
  • Patent number: 9179692
    Abstract: Embodiments of the process for isolating proteins from a defatted meal comprise extracting the defatted meal with an acidic solvent at a pH of between 2 and 6 to obtain a mixture of treated meal and a first solution of proteins. The process further comprises extracting the treated meal with an alkaline solvent at a pH of between 9 and 12.5 to obtain a mixture of meal residue and a second solution of proteins. The meal residue is separated from the second solution of proteins, and the proteins are separated from the second solution of proteins to obtain one or more protein products.
    Type: Grant
    Filed: June 9, 2009
    Date of Patent: November 10, 2015
    Inventors: Olev Trass, Eduard Tearo, Toivo Trass
  • Patent number: 9176310
    Abstract: One embodiment of techniques for confocal microscopy includes illuminating a spot on a surface of a biological sample. A first emission intensity from the spot is detected in a first range of optical properties; and a second emission intensity in a second range. A pixel that corresponds to the spot is colored using a linear combination of the first and second emission intensities. Sometimes, the pixel is colored to approximate a color produced by histology. In some embodiments, a surface of a sample is contacted with a solution of acridine orange. Then, a spot is illuminated with a laser beam of wavelength about 488 nanometers (nm). Fluorescence emission intensity is detected above about 500 nm. Sometimes, a certain illumination correction is applied. In some embodiments, a sample holder that compresses a sample is removable from a stage that is fixed with respect to a focal plane of the microscope.
    Type: Grant
    Filed: July 24, 2009
    Date of Patent: November 3, 2015
    Assignees: Sloan Kettering Institute for Cancer Research, Oregon Health & Science University
    Inventor: Daniel S. Gareau
  • Patent number: 9149529
    Abstract: Compositions comprising thrombin and collagen and methods of preparation thereof are disclosed herein. In one embodiment, a composition comprises thrombin and collagen in an aqueous buffer solution, wherein the buffer solution includes at least one of a first compound represented by Formula I: R1—C(O)—O?M+, wherein R1 is —CH3, —CH2CH2C(O)OH, or —CH2CH2C(O)O?M+, and wherein M+ is a monovalent ion; or a second compound represented by Formula II: R2—C(O)—O?M2+?O—C(O)—R2, wherein R2 is —CH3 or —CH2CH3, and wherein M2+ is a divalent ion. In one embodiment, the buffer solution includes sodium acetate, calcium propionate, and sorbitol.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: October 6, 2015
    Assignee: Orthovita, Inc.
    Inventor: Venkat R. Garigapati
  • Patent number: 9139855
    Abstract: The embodiments disclosed herein relate to bacterial cell production of triacylglycerides from renewable biomass resources.
    Type: Grant
    Filed: October 19, 2012
    Date of Patent: September 22, 2015
    Assignee: Massachusetts Institute of Technology
    Inventors: Kazuhiko Kurosawa, Anthony John Sinskey
  • Patent number: 9138416
    Abstract: The present invention is a composition composed of a therapeutic agent encapsulated in a copolymer of an N-alkylacrylamide, a vinyl monomer, and a polyethylene glycol (PEG) conjugate and a method for using the same in the treatment or prevention of a disease or condition.
    Type: Grant
    Filed: October 28, 2013
    Date of Patent: September 22, 2015
    Assignee: Board of Regents of the University of Nebraska by and behalf of the University of Nebraska Medical Center
    Inventors: Vinod D. Labhasetwar, Sanjeeb K. Sahoo, Maram K. Reddy
  • Patent number: 9134318
    Abstract: A diagnostic method for determining the absence or presence of a disease is provided. The method generally includes assaying the amount and/or types of oxidized peptides in a sample from a subject, and comparing these to the amount and types of reference oxidized polypeptides. The method may include the use of stable isotope label, affinity selection, to identify and quantify changes in oxidized peptides or oxidized proteins associated with diseases such as type II diabetes mellitus, breast cancer, and Parkinson's disease, to monitor a patient's response to a therapeutic agent (e.g., an antioxidant), and/or to monitor disease recurrence.
    Type: Grant
    Filed: December 10, 2010
    Date of Patent: September 15, 2015
    Assignee: Purdue Research Foundation
    Inventors: Ashraf G. Madian, Fred E. Regnier
  • Patent number: 9103817
    Abstract: The present invention is concerned with a method and apparatus for determining the instant at which coagulating blood forms a clot—the primary function of a clot being to act as a hemostatic plug at the site of a lesion in the circulatory system. The method comprises applying a harmonically time-varying stress and strain of controlled amplitude and frequency to the blood using a rheometer and through the use of the Chambon-Winter Gel Equation, the method is capable of determining the gel point which precedes the clotting time indicated by a thromboelastograph.
    Type: Grant
    Filed: April 20, 2006
    Date of Patent: August 11, 2015
    Assignee: Haemometrics Limited
    Inventors: Phylip Rhodri Williams, Adrian Evans
  • Patent number: 9089625
    Abstract: Disclosed herein is a composition suitable for use in spinal surgery. The composition generally includes a reaction product or settable mixture of first and second components, the first component including a spray-dried inorganic filler, a radical donor, and a pre-polymerized vinyl polymer, and the second component including a radical scavenger, a diluent, a polymerization accelerator, and a reactive monomer. Furthermore, disclosed herein is a method of making the composition, which generally includes mixing the first and second components under conditions suitable to form a curable reaction product.
    Type: Grant
    Filed: December 10, 2009
    Date of Patent: July 28, 2015
    Assignee: KYPHON SARL
    Inventors: Jörg Meyer, Robert Wenz
  • Patent number: 9078903
    Abstract: The present invention includes methods and compositions designed for treating a subject suffering from a skin condition, disorder or disease. The compositions include undifferentiated fetal skin cells that are either integrated with a collagen matrix or a carrier.
    Type: Grant
    Filed: January 15, 2013
    Date of Patent: July 14, 2015
    Assignee: MERZ GMBH & CO. KGAA
    Inventors: Lee Laurent-Applegate, Patrick Hohlfeld
  • Patent number: 9078429
    Abstract: A non-linear cooling cryopreservation method for improving cryopreservation protocols for cells that involves producing a simulation of cellular responses to a range of cooling parameters; determining optimal cooling parameters required to minimize cryoinjury to the cells using simulation of cellular responses and experimental results; and incorporating optimal parameters into the protocol. The simulation is based on mathematical models of cellular parameters. A non-linear cooling cryopreservation protocol for cryopreserving stem cells is also disclosed that does not require cryoprotectants.
    Type: Grant
    Filed: February 4, 2010
    Date of Patent: July 14, 2015
    Assignee: The Governors of the University of Alberta
    Inventors: Locksley Earl McGann, Janet Anne Wade Elliott, Lisa Ula Ross-Rodriguez
  • Patent number: 9063147
    Abstract: This invention provides methods of isolating ferritin from plant and animal material. The isolated ferritin can be administered to humans or animals in need of iron, and can be used to treat or supplement iron deficiency. The isolated ferritin can be used in industrial applications, such as increasing the iron content in heat-processed food or beverages. The methods of the invention also include quantitation of iron derived from plant or animal ferritin.
    Type: Grant
    Filed: June 3, 2013
    Date of Patent: June 23, 2015
    Assignee: SLO-IRON, LLC
    Inventor: Elizabeth C. Theil
  • Patent number: 9057088
    Abstract: Silver-coated proteins, being dissolvable or suspendable in aqueous media and/or retaining a biological activity of the protein and a process for preparing same are disclosed. Further disclosed are modified proteins which have a reducing moiety attached to the surface thereof and a process for preparing same. The modified proteins can be utilized for obtaining the silver-coated proteins. Further disclosed are a pharmaceutical composition containing and a method of treating bacterial and fungal infections utilizing biologically active silver-coated hydrogen peroxide producing enzymes such as glucose oxidase. Further disclosed are conductive elements that comprise the silver-coated proteins disclosed herein and electronic circuits containing same. Further disclosed are electrodes having the silver-coated proteins deposited thereon and biosensor systems utilizing same for determining a level of an analyte in a liquid sample.
    Type: Grant
    Filed: January 26, 2006
    Date of Patent: June 16, 2015
    Assignee: Ramot at Tel-Aviv University Ltd.
    Inventors: Amihay Freeman, Yosi Shacham-Diamand, Hila Moscovich-Dagan, Noa Hadar
  • Patent number: 9050332
    Abstract: The present invention relates to a composition comprising at least one biologically active protease and at least one biologically active glycosidase wherein the proteases and the glycosidases are present in an activity ratio of from 1,000,000:1 to 1:1,000,000 and wherein the composition has a total enzyme activity of at least 2 U/ml. Furthermore, the present invention relates to a process of producing such enzyme comprising composition and to processes of removing caries. Moreover, the invention relates to the use of one or more enzyme comprising compositions for producing a treatment agent for removing caries.
    Type: Grant
    Filed: May 16, 2008
    Date of Patent: June 9, 2015
    Assignee: 3M Innovative Properties Company
    Inventors: Ingo Häberlein, Rainer Guggenberger, Wolfgang Weinmann, Oliver Kappler
  • Patent number: 9051542
    Abstract: Compositions and methods are provided for preparation of concentrated stock solutions of AAV virions without aggregation. Formulations for AAV preparation and storage are high ionic strength solutions (e.g. ?˜500 mM) that are nonetheless isotonic with the intended target tissue. This combination of high ionic strength and modest osmolarity is achieved using salts of high valency, such as sodium citrate. AAV stock solutions up to 6.4×1013 vg/mL are possible using the formulations of the invention, with no aggregation being observed even after ten freeze-thaw cycles. The surfactant Pluronic® F68 may be added at 0.001% to prevent losses of virions to surfaces during handling. Virion preparations can also be treated with nucleases to eliminate small nucleic acid strands on virions surfaces that exacerbate aggregation.
    Type: Grant
    Filed: March 19, 2010
    Date of Patent: June 9, 2015
    Assignee: Genzyme Corporation
    Inventors: John Fraser Wright, Guang Qu
  • Patent number: 9017667
    Abstract: The therapeutic efficacy of zymogen proteins for oral administration is improved by including in the formulation one or more excipients that optimize pH and other reaction conditions for rapid activation of the zymogen shortly after ingestion.
    Type: Grant
    Filed: October 9, 2009
    Date of Patent: April 28, 2015
    Assignee: Alvine Pharmaceuticals, Inc.
    Inventor: Bret Berner
  • Patent number: 9012194
    Abstract: A method for producing sophorolipids having protein inducer and/or repressor activities having the steps of synthesizing the sophorolipid by fermentation of Candida bombicola in a fermentation media to form a natural mixture of lactonic sophorolipids and non-lactonic sophorolipids and then utilizing the natural mixture as a protein inducing agent, utilizing the natural mixture as a protein repressing agent, and/or utilizing the natural mixture as a combined protein induction/repressor agent. An application of the sophorolipid compound produced according to the method as a microbial media component.
    Type: Grant
    Filed: December 22, 2005
    Date of Patent: April 21, 2015
    Assignee: Synthezyme LLC
    Inventors: Richard A. Gross, Vishal Shah, Frantisek Nerud, Datta Madamwars
  • Patent number: 9012169
    Abstract: The invention is concerned with the identification of compounds as flavors or fragrances, their precursors, or as modulators of the perception of fragrances or flavors. The method comprises a reaction of a compound with a metabolic enzyme expressed in the nose, mouth or respiratory tract, followed by a method of identification of the compound or its metabolites as a fragrance or flavor, their precursor, or a modulator of their perception or of the perception of their respective leads.
    Type: Grant
    Filed: July 15, 2005
    Date of Patent: April 21, 2015
    Assignee: Givaudan S.A.
    Inventor: Boris Schilling
  • Patent number: 9011842
    Abstract: The present technology relates to an enzyme composition. The enzyme composition may be used to treat gluten intolerant subjects, including suffering from non-Celiac gluten intolerance and/or non-Celiac gluten sensitivity. The enzyme composition may also be used to reduce gluten exposure in certain individuals. For example, the enzyme composition may also be used as a prophylactic to reduce exposure to gluten oligopeptides.
    Type: Grant
    Filed: February 2, 2011
    Date of Patent: April 21, 2015
    Assignees: Amano Enzyme Inc., Amano Enzyme USA Co., Ltd.
    Inventors: James F. Jolly, Horoki Ido, Hirotaka Matsubara, Tetsuya Takahashi, Kyoichi Nishio
  • Patent number: 9012159
    Abstract: A method for identifying a compound which modulates the activity of acyl-coA: diacylglycerol acyltransferase comprises the steps of contacting a stable isotope labeled fatty acid with cells in either presence or absence of the compound, extracting the cells with isopropyl alcohol, and determining the level of a stable isotope labeled triglyceride in the presence or absence of the compound; wherein a change in the level of the stable isotope labeled triglyceride indicates that the compound modulates the DGAT activity.
    Type: Grant
    Filed: January 7, 2010
    Date of Patent: April 21, 2015
    Assignee: Janssen Research & Development, LLC
    Inventors: Jian-Shen Qi, Wensheng Lang, Margery A. Connelly
  • Patent number: 8993313
    Abstract: Provided are an analytical instrument and an analytical method that allow for the direct analysis of a target substance in an undiluted specimen by a transmission photometry in a tightly closed cell container space. An analytical instrument is an analytical instrument for analyzing a target substance contained in a specimen flown in the tightly closed cell container by utilizing an oxidative color-developing agent and an oxidative enzyme reaction, in which an upper substrate and a lower substrate are arranged facing each other and at least a part of the upper substrate and/or at least a part of the lower substrate are/is made of a material transmitting light used for the analysis and having oxygen transmission properties.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: March 31, 2015
    Assignee: ARKRAY, Inc.
    Inventor: Toshihiro Imai