Abstract: An improved chromogen-reagent test system for a slide, test kit or the like has particular utility in the testing of fecal specimens for occult blood. The chromogen-reagent system may include the use of colorless guaiac which, in the presence of a developing solution and a catalyst, is oxidized to yield a predetermined color such as blue. Hydrogen peroxide is used as the developing solution and hemoglobin in the fecal specimen functions as the catalyst. The chromogen-reagent test system includes an indicator area for determining if the guaiac and the developing solution are functioning properly, i.e., the test system of the present invention verifies that the guaiac has not lost its activity nor been improperly catalyzed and that the hydrogen peroxide has not lost its activity. The indicator area includes a catalyst which has a greater stability than blood, in respect to adverse environmental conditions, such that a determination can be made if the reagents are functioning properly.

Abstract: This invention relates to polypeptides that are human somatomedin carrier protein subunits and to processes for producing them. The carrier protein subunits bind to human somatomedin-like polypeptides, also known as insulin-like growth factors. The process involves preparation from a human serum fraction, Cohn IV-1, by a molecule of various chromatographic steps.This invention also relates to DNA molecules encoding human somatomedin carrier protein-like polypeptides, recombinant DNA molecules, hosts, processes for producing carrier protein-like polypeptides, human somatomedin carrier protein-like polypeptides produced using those molecules, hosts and processes. The invention relates to DNA molecules and their expression in appropriate hosts. The recombinant DNA molecules contain DNA molecules that code for polypeptides which have a biological activity of the human carrier protein or a human carrier protein subunit capable of binding somatomedins.

Abstract: A novel approach to study changes in protein tyrosine phosphorylation during apoptosis, and thereby identify cells committed to apoptosis is presented, methods used to study apoptosis and tyrosine phosphorylation at the single cell level are combined to study directly whether apoptosis in hematopoietic cells is associated with changes in cellular phosphotyrosine levels. The changes in cellular phosphotyrosine content strongly correlated with the appearance of features of cell death such as cell shrinkage, DNA fragmentation and loss of membrane integrity.