Patents Examined by Sean C Barron
  • Patent number: 12365868
    Abstract: The present disclosure provides methods and systems for production of biostimulants that promote nitrogen use efficiency in plants. Embodiments described include methods of making a biostimulant composition in a bioreactor system that includes two or more containers arranged in series. The bioreactor system may include an established population of a nitrogen use efficiency-promoting microbial strain. The method may include operating the bioreactor system by transferring into the system an aqueous feedstock that comprises a microbial consortium, transferring working fluid between containers of the system, and collecting a product. The method may further include maintaining a concentration of a nitrogen use efficiency-promoting microbial strain in the system.
    Type: Grant
    Filed: December 15, 2023
    Date of Patent: July 22, 2025
    Assignee: TENFOLD TECHNOLOGIES, LLC
    Inventors: Luis Francisco Velazquez, Allana Kay Welsh, Neissa Maryann Pinzon, Maud Ann Wrightson Hinchee, Robert D Chisholm, Mohammad Kamrul Hassan, Leslie Michelle Perry, Shashi S Rajbanshi, Katherine McElhany Williams
  • Patent number: 12359238
    Abstract: A polypeptide comprising a residue substituted by a non-canonical amino acid (ncAA) is provided. Methods for: (a) transferring an electron to an electrode, by coupling the polypeptide to an electrode; and (b) quantifying the amount of an analyte e.g., glucose are also provided.
    Type: Grant
    Filed: May 27, 2021
    Date of Patent: July 15, 2025
    Assignee: B. G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITY
    Inventors: Lital Alfonta, Raz Zarivach, Jennifer Grushka, Itay Algov
  • Patent number: 12359226
    Abstract: Provided herein is an isolated Methanothermobacter microorganism that is (a) a microorganism of Methanothermobacter thermautotrophicus strain UC 120910, deposited on Dec. 21, 2010, with the American Type Culture Collection (ATCC) under ATCC® Patent Deposit Designation No. PTA-11561, (b) a variant of the microorganism of Methanothermobacter thermautotrophicus strain UC 120910, or (c) a progeny of the microorganism of Methanothermobacter thermautotrophicus strain UC 120910, wherein the variant or progeny retains the phenotypic characteristics of the microorganism of Methanothermobacter thermautotrophicus strain UC 120910. Also provided herein is a substantially pure culture or monoculture comprising the Methanothermobacter microorganism of the disclosure.
    Type: Grant
    Filed: February 16, 2022
    Date of Patent: July 15, 2025
    Assignee: THE UNIVERSITY OF CHICAGO
    Inventor: Laurens Mets
  • Patent number: 12350401
    Abstract: Provided are a composition and a sheet, including a mesenchymal stem cells-hydrogel-biodegradable support or a mesenchymal stem cells-hydrogel-nondegradable support and a preparing method thereof. More specifically, in the sheet including a mesenchymal stem cells-hydrogel-biodegradable support or a mesenchymal stem cells-hydrogel-nondegradable support according to the present invention, the high-active mesenchymal stem cells may be applied to a wounded part of a patient with epidermolysis bullosa as it is without isolation using proteases, and in the culturing, an extracellular matrix such as collagen, laminin, fibronectin, and elastin secreted from the mesenchymal stem cells is wholly present on the hydrogel to have an advantageous effect that skin reproduction and re-epithelization abilities are significantly excellent as compared with conventional dressing agents used for epidermolysis bullosa.
    Type: Grant
    Filed: August 11, 2022
    Date of Patent: July 8, 2025
    Assignee: ANTEROGEN CO., LTD.
    Inventors: Sung-Koo Lee, Mihyung Kim
  • Patent number: 12351819
    Abstract: The present invention relates to a method for removing undifferentiated embryonic stem cells. In particular, the present invention relates to a method for removing undifferentiated embryonic stem cells by using cardiac glycosides (CGs). The present invention also relates to a method for preparing differentiated cells in which undifferentiated embryonic stem cells have been removed and a method for cell therapy by using such differentiated cells.
    Type: Grant
    Filed: June 6, 2018
    Date of Patent: July 8, 2025
    Assignee: ACADEMIA SINICA
    Inventors: Joyce Jean Lu, Yu-Tsen Lin
  • Patent number: 12270739
    Abstract: A sectionable tissue sample support structure including a gel compound formed into a self supporting geometric shape for retention and orientation of at least one tissue sample during a histopathology process including processing, embedding and microtome slicing of the tissue sample. A method of orienting, processing, embedding and microtome slicing a tissue sample using a gel compound preformed into a self supporting geometric shape. A combination including the sectionable tissue sample support structure and a package containing the sectionable tissue sample support structure.
    Type: Grant
    Filed: September 26, 2023
    Date of Patent: April 8, 2025
    Assignees: BIOPATH AUTOMATION, LLC, SAKURA FINETEK U.S.A., INC.
    Inventors: Warren P. Williamson, IV, Richard W. Dapson, Amit D. Shah
  • Patent number: 12264305
    Abstract: A method and device for isolating a migratory cell population from a mixed population of migratory cells and non-migratory cells is described herein. The method comprises placing the mixed population of migratory cells and non-migratory cells in contact with a device having one or more microchannels; and isolating the migratory cell population within the microchannels following an incubation period, wherein the one or more microchannels have an average size in a proliferation direction of the migratory cells that is less than a minimum proliferation space of the migratory cells along the proliferation direction.
    Type: Grant
    Filed: September 18, 2019
    Date of Patent: April 1, 2025
    Assignees: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, THE BOARD OF REGENTS OF THE UNIVERSITY OF OKLAHOMA
    Inventors: Young-tae Kim, Loan Bui, James Battiste, Xue Cai, Michael Sughrue
  • Patent number: 12264355
    Abstract: The present invention relates to a process for preparing ammonium (meth-) acrylate, aqueous ammonium (meth-) acrylate solutions obtainable by such process, and (meth-) acrylic acid homopolymers or copolymers obtainable by polymerizing such ammonium (meth-) acrylate. The invention furthermore relates to a modular, relocatable bioconversion unit for manufacturing aqueous ammonium (meth-) acrylate solutions.
    Type: Grant
    Filed: October 17, 2019
    Date of Patent: April 1, 2025
    Assignee: BASF SE
    Inventors: Diego Ghislieri, Peter Oedman, Tobias Joachim Zimmermann, Anna-Corina Schmidt
  • Patent number: 12252726
    Abstract: The present invention relates to a process for simultaneous production of citric acid and cellulolytic enzymes. The batch process comprising (i) adding slurry of a pre-treated lignocellulosic biomass or cellulose in a fermentation media; (ii) inoculating 10% (v/v) active liquid seed culture of Penicillium funiculosum MRJ-16 in the fermentation media of step (i); (iii) subjecting the culture of step (ii) to fermentation in an aerated fermenter at a temperature of 28-33° C. for a duration of 96 hours; and (iv) collecting enzyme broth after fermentation of step (iii) to obtain citric acid and cellulolytic enzymes. The batch and fed-batch process of the present invention results in high titer production of citric acid and cellulolytic enzymes in a single step and using single microbial strain.
    Type: Grant
    Filed: July 31, 2021
    Date of Patent: March 18, 2025
    Assignee: INDIAN OIL CORPORATION LIMITED
    Inventors: Mukund Adsul, Simranjeet Kaur Sandhu, Reeta Rani Singhania, Anshu Shankar Mathur, Ravi Prakash Gupta, Deepak Kumar Tuli, Suresh Kumar Puri, Sankara Sri Venkata Ramakumar
  • Patent number: 12227780
    Abstract: The present invention relates to methods for producing isothiocyanate containing products from Brassicaceae material and lactic acid bacteria for use in such methods. The present invention also relates to isothiocyanate containing products from Brassicaceae material produced by such methods.
    Type: Grant
    Filed: September 27, 2018
    Date of Patent: February 18, 2025
    Assignee: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION
    Inventors: Mary Ann Augustin, Netsanet Shiferaw Terefe
  • Patent number: 12227775
    Abstract: Described herein are genetically engineered template-independent DNA polymerases, specifically terminal deoxynucleotidyl transferases, and methods of using these polymerases to control DNA synthesis by adding a single nucleotide (mononucleotide) at a time to the 3? end of a growing single-stranded DNA polynucleotide.
    Type: Grant
    Filed: May 22, 2018
    Date of Patent: February 18, 2025
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Andrew P. Magyar, Peter Cavanagh, Miles Rogers, Kirsty A. McFarland, Melissa M. Sprachman, Amanda Nicole Billings-Siuti, Nicole E. Raustad
  • Patent number: 12221623
    Abstract: Methods for processing and sorting sperm are disclosed. Portions of sperm sorting or staining processes may include standardizing sperm samples by adjusting the concentration of the sperm sample to a predetermined concentration and adjusting the pH of the sample to a predetermined value. Sperm may also be stained in a single staining buffer having a DNA selective dye and a quenching dye.
    Type: Grant
    Filed: March 4, 2013
    Date of Patent: February 11, 2025
    Assignee: INGURAN, LLC
    Inventors: Thomas Boyd Gilligan, Kenneth Michael Evans, Richard Lenz, Clara Gonzalez-Marin, Ramakrishnan Vishwanath
  • Patent number: 12214000
    Abstract: The present invention relates to a method for isolating stem cells comprising preparing a cell suspension from uterine cervix tissue, to the stem cells isolated by said method, and to the conditioned medium obtained from the culture of said stem cells. The invention also encompasses the use of said stem cells or conditioned medium for treating or preventing cancer, precancerous lesions, inflammatory diseases, autoimmune diseases, chronic pathologies or infectious diseases, diseases associated to tissue loss, or for use in diagnostic, prognostic or treatment of fertility disorders, as well as for cosmetic treatment.
    Type: Grant
    Filed: April 9, 2021
    Date of Patent: February 4, 2025
    Assignee: GISTEM RESEARCH S.L.
    Inventors: Francisco José Vizoso Piñeiro, Román Pérez Fernández, Noemí Eiró Díaz
  • Patent number: 12195781
    Abstract: A process is provided for management of ethanol concentration during syngas fermentation. A process for fermentation of syngas includes inoculating a medium to provide an inoculated medium. Inoculated medium is contacted with syngas and cells and medium are removed and separated to provide concentrated cells and permeate. Ethanol is separated from the permeate to provide ethanol and a reduced ethanol aqueous stream. The reduced ethanol aqueous stream is returned to the fermentation.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: January 14, 2025
    Inventors: Ryan Senaratne, Song Liu
  • Patent number: 12195708
    Abstract: To provide a means capable of providing excellent cell adhesion to a polymer substrate (particularly, a hydrophilic polymer substrate) as compared to the case of performing a surface treatment with polytetrahydrofurfuryl acrylate (PTHFA). Provided is a cell culture substrate comprising a coating layer on at least one side of a polymer substrate, wherein the coating layer includes a copolymer having more than 40% by mole and less than 100% by mole of a structural unit derived from furfuryl (meth)acrylate represented by Formula and more than 0% by mole and less than 60% by mole of a structural unit derived from ethylenically unsaturated monomer having a carboxylic group (the total of the structural unit and the structural unit is 100% by mole).
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: January 14, 2025
    Assignee: TERUMO KABUSHIKI KAISHA
    Inventors: Takao Anzai, Ichiro Hirahara
  • Patent number: 12188047
    Abstract: A method of generating retinal pigment epithelium cells is disclosed. Cell populations comprising same and uses thereof are also disclosed.
    Type: Grant
    Filed: October 26, 2016
    Date of Patent: January 7, 2025
    Assignees: HADASIT MEDICAL RESEARCH SERVICES AND DEVELOPMENT LTD., CELL CURE NEUROSCIENCES LTD.
    Inventors: Benjamin Eithan Reubinoff, Orna Singer, Osnat Bohana-Kashtan, Ofer Wiser
  • Patent number: 12144908
    Abstract: Disclosed are cell encapsulation devices and methods for transplanting cells, such as pancreatic endoderm cells, into a host. In some examples, a cell encapsulation can comprise a lumen configured to receive cells therein, a cell-excluding membrane, where the lumen is internal to the cell-excluding membrane, and a non-woven fabric layer external to the cell-excluding membrane, where the non-woven fabric layer and the cell-excluding membrane comprise perforations. The device can further comprise a woven mesh external to the non-woven fabric layer, where the non-woven fabric layer provides protection to the cell-excluding membrane from direct contact with the woven mesh.
    Type: Grant
    Filed: March 2, 2023
    Date of Patent: November 19, 2024
    Assignee: ViaCyte, Inc.
    Inventors: Kevin Allen D'Amour, Evert Kroon, Michael Scott, Laura Martinson, Craig McGreevy
  • Patent number: 12139741
    Abstract: The present disclosure relates to biocide compositions compatible with enzyme biosensors and methods for using same. More particularly, the disclosure relates to biocide compositions compatible with enzymes used for measuring creatine and creatinine levels.
    Type: Grant
    Filed: October 25, 2019
    Date of Patent: November 12, 2024
    Assignee: Instrumentation Laboratory Company
    Inventors: Dennis Robert Conlon, Prasad Pamidi
  • Patent number: 12129506
    Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.
    Type: Grant
    Filed: June 29, 2022
    Date of Patent: October 29, 2024
    Assignee: CARGILL, INCORPORATED
    Inventors: Michael D. Lynch, Michael Tai Man Louie, Shelley Copley, Eileen Colie Spindler, Brittany Robinson, Matthew Lipscomb, Tanya Lipscomb-Warnecke, Hans H. Liao, David Hogsett, Ron Evans
  • Patent number: 12115492
    Abstract: The present disclosure relates to processes for desulfurizing hydrocarbon feedstocks. The processes may include introducing a feedstock comprising hydrogen sulfide to an absorber comprising a metal chelate to form a reduced metal chelate. The processes may further include introducing the reduced metal chelate to a photobioreactor comprising a phototrophic bacterium. The present disclosure also relates to apparatuses for desulfurizing hydrocarbon feedstock. An apparatus may include and absorber and a photobioreactor fluidly connected to the absorber. The photobioreactor may be an anaerobic vessel with a light source.
    Type: Grant
    Filed: October 28, 2020
    Date of Patent: October 15, 2024
    Assignee: ExxonMobil Technology and Engineering Company
    Inventors: Mark L Heinnickel, Steven W Levine, Robert D Nielsen, Yuchen Liu, Joseph C Weissman