Patents Examined by Sean C Barron
  • Patent number: 11447733
    Abstract: The present invention relates to transparent disc-shaped microparticles having polydopamine-modified surfaces and cell culture using the same. When the disc-shaped microparticles coated with polydopamine according to the present invention are used in cell culture, cell adhesive properties are improved and an inverted microscope may be employed to observe cells, which are major advantages when compared to conventional spherical microcarriers.
    Type: Grant
    Filed: January 15, 2018
    Date of Patent: September 20, 2022
    Inventors: Tong In Oh, Sung Hyun Kim, Wook Park, Eun Ah Lee
  • Patent number: 11439668
    Abstract: Methods are described for differentiating stem and post-natal cells into sex hormone-producing cells that can be administered to a patient autologously or allogeneically in order to maintain in balance, or rebalance, their hypothalamic-pituitary-gonadal (HPG) axis.
    Type: Grant
    Filed: April 6, 2018
    Date of Patent: September 13, 2022
    Assignee: JangoBio, LLC
    Inventors: Craig S. Atwood, Sivan Vadakkadath Meethal
  • Patent number: 11439730
    Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
    Type: Grant
    Filed: December 6, 2019
    Date of Patent: September 13, 2022
    Inventors: Chung-Hsing Chou, Dueng-Yuan Hueng, Tsung-Neng Tsai
  • Patent number: 11414635
    Abstract: Disclosed are fluidic devices and methods for preparing fluidic devices. More particularly, disclosed are fluidic devices having fiber scaffolds and methods for their preparation. Also disclosed are methods for culturing cells using the fluidic devices having fiber scaffolds.
    Type: Grant
    Filed: April 18, 2017
    Date of Patent: August 16, 2022
    Assignee: Saint Louis University
    Inventors: Robert Scott Martin, Chengpeng Chen, Scott Allen Sell
  • Patent number: 11408013
    Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.
    Type: Grant
    Filed: June 26, 2019
    Date of Patent: August 9, 2022
    Inventors: Michael Lynch, Michael Louie, Shelley Copley, Eileen Spindler, Brittany Robinson, Matthew Lipscomb, Tanya Lipscomb-Warnecke, Hans Liao, David Hogsett, Ron Evans
  • Patent number: 11408023
    Abstract: Devices for microbial detection of microorganisms are provided including a body member including a substrate having a first major surface and a second major surface. The device further includes a substantially dry, first microbial growth nutrient composition disposed on a portion of the first major surface, a first adhesive composition adhered to the first microbial growth nutrient composition, and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition. The device also includes a cover sheet attached to the body member, where the cover sheet includes a first major surface facing the body member. Devices including a water-proof pouch are also provided. Methods for detecting and enumerating at least one microorganism in a sample are additionally provided, using the devices.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: August 9, 2022
    Assignee: 3M Innovative Properties Company
    Inventors: Alexi J. Young, Evan D. Brutinel
  • Patent number: 11401500
    Abstract: A system and method for generating an incubated bacteria solution by heating a nutrient germinant composition and bacteria, including at least one species in spore form, to a preferred temperature a range of 35-50° C. for 2-60 minutes using exothermic chemical reaction heat. An incubated bacteria solution is preferably generated at or near a point-of-use in an aquaculture, agriculture, wastewater, or environmental remediation application. The nutrient-germinant composition comprises L-amino acids, optionally D-glucose and/or D-fructose, a buffer, an industrial preservative, and may include bacteria spores (preferably of one or more Bacillus species) or they may be separately combined for incubation. A first chemical contained in a pouch is activated by contact with a second chemical, water, or air in a flameless heater to initiate exothermic reaction to provide incubation heat.
    Type: Grant
    Filed: August 23, 2019
    Date of Patent: August 2, 2022
    Assignee: NCH Corporation
    Inventors: Charles Greenwald, Ivy Jones, John Knope
  • Patent number: 11400139
    Abstract: Embodiments of the technology described herein are based, in part, upon the discovery that NETosis, the formation of neutrophil extracellular traps (NETs) is increased in wounds, in organ fibrosis and in subjects with diabetes. Accordingly, methods for treating wounds, fibrosis and NET associated complications in diabetes are provided. The methods comprise administrating a therapeutically effective amount of at least one anti-NET compound to a subject in need of treatment, e.g. a PAD 4 inhibitor, a DNase, a histone-degrading enzyme; an inhibitor of chromatin decondensation; an antibody against a component of a NET; an inhibitor of NET release, a protease inhibitor, or an elastase inhibitor.
    Type: Grant
    Filed: January 19, 2016
    Date of Patent: August 2, 2022
    Assignee: The Children's Medical Center Corporation
    Inventors: Denisa D. Wagner, Siu Ling Wong, Kimberly Lindsay Martinod, Luise Erpenbeck, Jörn Thilo Witsch, Alexander Savchenko
  • Patent number: 11390845
    Abstract: Provided is a method for manufacturing cell-containing blocks having steps of: preparing an standardized size mold by 3D printing (three dimensional printing) using a biocompatible elastic material; injecting a thermosensitive colloid into the mold to form a thermosensitive mold; injecting a hydrogel containing cells in to the thermosensitive mold and curing the hydrogel containing cells to form the cell-containing blocks; separating the thermosensitive mold and the cell-containing blocks at a temperature higher than a solidifying point of the thermosensitive colloid. Also provided are method for assembling the cell-containing blocks in a target configuration by using an assembling mold defining the target configuration and made of a thermoreversible material.
    Type: Grant
    Filed: June 20, 2019
    Date of Patent: July 19, 2022
    Assignee: Asia University
    Inventors: Yu Fang Shen, Ming You Shie, Yi Wen Chen, Wei Huang Wang
  • Patent number: 11382958
    Abstract: The present invention relates to debriding compositions in the form of an aqueous gel. Particularly, the present invention relates to a debriding composition comprising a proteolytic enzyme mixture obtained from bromelain present in a dry form, and an aqueous gel carrier, wherein, prior to use, the proteolytic enzyme mixture being admixed with the aqueous gel carrier to form a debriding composition useful for debridement and treatment of c wounds.
    Type: Grant
    Filed: January 30, 2017
    Date of Patent: July 12, 2022
    Assignee: MediWound Ltd.
    Inventors: Eilon Asculai, Dafna Geblinger, Mery Kleyman, Deborah Hanah Bartfeld
  • Patent number: 11359174
    Abstract: The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.
    Type: Grant
    Filed: August 23, 2019
    Date of Patent: June 14, 2022
    Assignee: Ecovative Design LLC
    Inventors: Jacob Michael Winiski, Peter James Mueller
  • Patent number: 11345942
    Abstract: The invention relates to solid medium for producing glucosamine, including a substrate and 0.15-4.8 mL/g substrate of supplemental solution, which includes 0.1-2 g/L KH2PO4, 0.1-2 g/L NaCl, and 0.1-2 g/L MgSO4.7H2O. The invention also relates to a method for producing glucosamine, including providing microorganism being able to produce glucosamine, and fermenting the microorganism in the medium mentioned above.
    Type: Grant
    Filed: September 6, 2019
    Date of Patent: May 31, 2022
    Inventors: Ho-Shing Wu, Jia-Wei Peng
  • Patent number: 11345881
    Abstract: The present disclosure relates to a nanofiber structure for cell culture, a method for manufacturing the structure, and a cell analysis device including the nanofiber structure for cell culture. The structure includes a cell culture layer made of nanofibers; and a spacer protruding upward from a surface of the cell culture layer, wherein the spacer divides a region on the cell culture layer into at least two culturing regions, wherein the spacer is made of the same nanofibers as the cell culture layer and thus has a cell migration channel defined therein.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: May 31, 2022
    Inventors: Jong-Young Kwak, Young-Hun Jeong, Jeong-Hwa Kim, Tae-Eon Kim
  • Patent number: 11338120
    Abstract: Methods for treating a subject for a parasympathetic bias mediated condition are provided. Aspects of the methods include modulating at least a portion of the subject's autonomic nervous system to increase the sympathetic/parasympathetic activity ratio in a manner effective to treat the subject for the parasympathetic bias mediated condition. In some instances, the subject is known to have parasympathetic bias. Also provided are devices that find use in practicing various embodiments of the methods.
    Type: Grant
    Filed: April 15, 2019
    Date of Patent: May 24, 2022
    Assignee: Palo Alto Investors LP
    Inventors: Anthony Joonkyoo Yun, Jeremy Thomas Yun, Eric Foster Yun
  • Patent number: 11338065
    Abstract: Engineered human tissue seed constructs are provided that are suitable for implantation in subjects. Methods of making and using the engineered tissue seed constructs are provided.
    Type: Grant
    Filed: October 7, 2016
    Date of Patent: May 24, 2022
    Assignees: Massachusetts Institute of Technology, Trustees of Boston University
    Inventors: Sangeeta N. Bhatia, Kelly R. Stevens, Christopher S. Chen
  • Patent number: 11339411
    Abstract: The invention is directed to a process to prepare elemental sulphur by (i) contacting an aqueous solution comprising bisulphide with oxidised sulphide-oxidising bacteria under anaerobic conditions wherein elemental sulphur is produced and a reduced sulphide-oxidising bacteria is obtained and (ii) wherein the reduced sulphide-oxidising bacteria are oxidised by transfer of electrons to an anode of an electrochemical cell to obtain the oxidised sulphide-oxidising bacteria.
    Type: Grant
    Filed: May 30, 2018
    Date of Patent: May 24, 2022
    Assignee: Paqell B.V.
    Inventors: Johannes Bernardus Maria Klok, Annemiek Ter Heijne, Frederikus De Rink, Cees Jan Nico Buisman
  • Patent number: 11319573
    Abstract: A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a ?-D-glucuronidase indicator system that comprises a plurality of compounds that enhance ?-glucuronidase enzyme activity in E. coli, a redox indicator system and a first cold-water soluble gelling agent adhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. Methods of using the device are also provided.
    Type: Grant
    Filed: April 2, 2018
    Date of Patent: May 3, 2022
    Inventors: Sailaja Chandrapati, Alec J. Teagarden, Haley A. Saddoris
  • Patent number: 11286449
    Abstract: The purpose of the present invention is to provide a cell culture substratum which has excellent resistance to liquid culture media and low cytotoxicity, can achieve a high cell adhesion ratio and a high viability of cultured cells, has excellent thermal stability, and is less likely to absorbs ultraviolet ray.
    Type: Grant
    Filed: May 18, 2017
    Date of Patent: March 29, 2022
    Inventors: Masahiko Inui, Sunao Chatani, Motohiro Tagaya, Satoshi Motozuka
  • Patent number: 11266766
    Abstract: Engineered human tissue seed constructs are provided that are suitable for implantation in subjects. Methods of making and using the engineered tissue seed constructs are provided.
    Type: Grant
    Filed: March 26, 2021
    Date of Patent: March 8, 2022
    Assignees: Massachusetts Institute of Technology, Trustees of Boston University
    Inventors: Sangeeta N. Bhatia, Kelly R. Stevens, Christopher S. Chen
  • Patent number: 11261476
    Abstract: The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.
    Type: Grant
    Filed: October 19, 2017
    Date of Patent: March 1, 2022
    Assignee: Q-LINEA AB
    Inventors: Markus Klintstedt, Harer Osman, Mats Gullberg