Patents Examined by Shafiqul Haq
  • Alkynyl sugar analogs for labeling and visualization of glycoconjugates in cells
    Patent number: 10317393
    Abstract: Methods for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates are disclosed. Alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars are incorporated into cellular glycoconjugates. Chemical probes comprising an azide group and a visual or fluorogenic probe and used to label alkyne-derivatized sugar-tagged glycoconjugates are disclosed. Chemical probes bind covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and are visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA, confocal microscopy, and mass spectrometry.
    Type: Grant
    Filed: October 10, 2017
    Date of Patent: June 11, 2019
    Assignee: ACADEMIA SINICA
    Inventors: Chi-Huey Wong, Tsui-Ling Hsu, Sarah R. Hanson, Masaaki Sawa
  • Controlling the architecture, coordination, and reactivity of nanoparticle coating utilizing an amino acid central scaffold
    Patent number: 10294213
    Abstract: A series of multicoordinating and multifunctional ligands optimized for the surface-functionalization of luminescent quantum dots (QDs) and gold nanoparticles (AuNPs) alike is disclosed. An L-aspartic acid precursor is modified with functionality, through simple peptide coupling chemistry, one or two lipoic acid (LA) groups and poly(ethylene glycol) (PEG) moieties in the same ligand. These ligands were combined with a new photoligation strategy to yield hydrophilic and reactive QDs that are colloidally stable over a broad range of conditions, including storage at nanomolar concentration and under ambient conditions.
    Type: Grant
    Filed: December 1, 2016
    Date of Patent: May 21, 2019
    Assignee: The Florida State University Research Foundation, Inc.
    Inventors: Hedi Mattoussi, Naiqian Zhan, Goutam Palui
  • Antibodies to quetiapine and use thereof
    Patent number: 10288631
    Abstract: Disclosed is an antibody which binds to quetiapine, which can be used to detect quetiapine in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of quetiapine, including multiplex detection of aripiprazole, quetiapine, olanzapine, and risperidone in a single lateral flow assay device.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: May 14, 2019
    Assignee: JANSSEN PHARMACEUTICA NV
    Inventors: Eric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Bart M. Remmerie, Rhys Salter, Matthew G. Donahue, Yong Gong
  • Composition for preparing biomaterial with excellent light-transmitting property, and use thereof
    Patent number: 10267714
    Abstract: The present invention provides an aminoalcohol together with, as necessary, at least one selected from the group consisting of: at least one compound selected from the group consisting of urea and a urea derivative; a nonionic detergent; and sucrose to provide a biological material having an excellent light-transmitting property. The present invention provides a technique that allows a high-throughput observation of gene expression/localization or cell morphology in tissue at an whole-tissue level.
    Type: Grant
    Filed: August 5, 2014
    Date of Patent: April 23, 2019
    Assignee: RIKEN
    Inventors: Etsuo Susaki, Hiroki Ueda, Kazuki Tainaka
  • Paramagnetic supports for use as assay reagents
    Patent number: 10259969
    Abstract: A composition for use as an assay reagent includes a paramagnetic solid support comprising a coating of a synthetic copolymer. The synthetic copolymer comprises two or three of a first copolymerized monomer, a second copolymerized monomer and a third copolymerized monomer and further comprises a polyethylenic backbone.
    Type: Grant
    Filed: August 23, 2018
    Date of Patent: April 16, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Bhalchandra S. Lele, Eric Allgaier, Robert Pervere, Jeremy Lyons, Steve St-Onge
  • Method and system for detection of natural high intensity sweeteners that contain hydroxyl groups
    Patent number: 10254278
    Abstract: The present invention provides a hapten derivative and conjugate of a natural high intensity sweetener containing hydroxyl groups. The conjugate can be used to produce antibodies specific against the natural high intensity sweetener. The present invention further provides a kit and method for detecting and quantifying the natural high intensity sweetener in a sample.
    Type: Grant
    Filed: August 22, 2016
    Date of Patent: April 9, 2019
    Assignee: PureCircle Sdn Bhd
    Inventors: Jose Antonio Gabaldon Hernandez, Estrella Nunez Delicado, Rosa Puchades Pla, Angel Maquiera Catala, Eva Maria Brun Sanchez, Avetik Markosyan
  • Solution for dissociating vitamin D from vitamin D-binding protein, associated detection method and use
    Patent number: 10222389
    Abstract: At least one fluoroalkyl surfactant and of at least one alcohol having 1 to 4 carbon atoms are used for dissociating vitamin D and/or a vitamin D metabolite from vitamin D binding protein. A solution comprising the at least one fluoroalkyl surfactant and at least one alcohol is provided. A method for detecting and quantifying, in vitro, vitamin D and/or at least one vitamin D metabolite in a biological sample includes treating the sample by incorporating at least one fluoroalkyl surfactant and at least one alcohol having 1 to 4 carbon atoms, so as to dissociate the vitamin D and/or its metabolite(s) to be detected from vitamin D binding protein; and detecting and quantifying vitamin D and/or at least one of its metabolites, in particular by immunoassay. A kit for detecting and quantifying vitamin D and/or at least one vitamin D metabolite by immunoassay and including the solution is provided.
    Type: Grant
    Filed: September 16, 2014
    Date of Patent: March 5, 2019
    Assignee: BIOMERIEUX
    Inventors: Valerie Meunier, Emmanuel Moreau
  • Silane mixtures
    Patent number: 10213762
    Abstract: Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: February 26, 2019
    Assignee: AFFYMETRIX, INC.
    Inventors: Glenn H. McGall, Robert G. Kuimelis
  • Nanotube array for optical detection of protein-protein interactions
    Patent number: 10215752
    Abstract: A composition can include a nanostructure, and a linker associated with the nanostructure, wherein the linker is configured to interact with a capture protein. The nanostructure can include a single-walled carbon nanotube. A plurality of the compositions can be configured in an array.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: February 26, 2019
    Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Michael S. Strano, Jin-Ho Ahn, Jong-Ho Kim, Paul W. Barone, Nigel F. Reuel
  • Platinum-labeled probes for mass cytometry
    Patent number: 10215758
    Abstract: The present disclosure provides analyte-specific binding reagents conjugated with a platinum-containing moiety, e.g., cisplatin, and methods, compositions, and kits for their production and use in assays for analyte detection.
    Type: Grant
    Filed: August 8, 2016
    Date of Patent: February 26, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Holden Terry Maecker, Michael Duane Leipold, Henrik Mei
  • Composition for preparing biomaterial with excellent light-transmitting property, and use thereof
    Patent number: 10209164
    Abstract: The present invention provides an aminoalcohol together with, as necessary, at least one selected from the group consisting of: at least one compound selected from the group consisting of urea and a urea derivative; a nonionic detergent; and sucrose to provide a biological material having an excellent light-transmitting property. The present invention provides a technique that allows a high-throughput observation of gene expression/localization or cell morphology in tissue at an whole-tissue level.
    Type: Grant
    Filed: August 5, 2014
    Date of Patent: February 19, 2019
    Assignee: RIKEN
    Inventors: Etsuo Susaki, Hiroki Ueda, Kazuki Tainaka
  • Stabilized liquid formulations containing receptors
    Patent number: 10203338
    Abstract: Methods and reagents are disclosed for preparing a liquid solution of a receptor. The methods comprise combining in a liquid medium the receptor, a chelating agent and a C2-C6 polyol. An amount of the chelating agent and the C2-C6 polyol is sufficient to achieve a stable and active receptor in the liquid solution, which is maintained at a temperature of about 2° C. to about 40° C. The compositions may be employed in assays for the determination of analytes that include receptor-binding analytes.
    Type: Grant
    Filed: May 13, 2014
    Date of Patent: February 12, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: David Kiaei
  • Compounds and methods for use in detecting gabapentin
    Patent number: 10203345
    Abstract: Compounds and methods for use in detecting gabapentin in a sample suspected of containing gabapentin are disclosed. Gabapentin derivatives are used to produce gabapentin conjugates. A gabapentin-immunogenic carrier conjugate may be used as an immunogen for the preparation of an anti-gabapentin antibody. A gabapentin-detectable label may be used in a signal producing system in gabapentin assays.
    Type: Grant
    Filed: November 11, 2016
    Date of Patent: February 12, 2019
    Assignee: ARK Diagnostics, Inc.
    Inventors: Johnny Jose Valdez, Byung Sook Moon, Michael Kevin Helms, Alejandro A. Orozco
  • Compositions and methods for detecting vitamin D
    Patent number: 10203344
    Abstract: Compounds include carbamate derivatives of vitamin D including vitamin D3 and vitamin D2. The compounds are useful in methods and kits for determining the presence and/or amount of vitamin D including vitamin D analogs and metabolites thereof in a sample suspected of containing the same.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: February 12, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Zhu Teng, Roland Janzen, Martin A. Drinan
  • Ceramides and their use in diagnosing CVD
    Patent number: 10197582
    Abstract: The present invention inter alia provides a method, and use thereof, of predicting CV complications such as AMI, ACS, stroke, and CV death by determining the concentrations of at least one ceramide of Group A and at least one ceramide of Group B in a biological sample and comparing those concentrations to a control. Finding a decreased concentration of at least one Group A ceramide and an increased concentration of at least one Group B ceramide indicates that the subject has an increased risk of developing one or more CV complications. Also provided are a newly identified subset of ceramide molecules, labelled versions thereof, and kits and compositions comprising the same for use in predicting and/or diagnosing CV complications.
    Type: Grant
    Filed: May 19, 2016
    Date of Patent: February 5, 2019
    Assignee: ZORA BIOSCIENCES, OY
    Inventor: Reijo Laaksonen
  • Accurate assay measurement of hydrophobic haptenic analytes
    Patent number: 10191069
    Abstract: Methods are disclosed for determining an actual concentration of a hydrophobic haptenic analyte in an unknown sample suspected of containing the hydrophobic haptenic analyte, wherein the unknown sample is suspected of containing an interfering substance. A first assay method is conducted on an unknown sample to obtain a measured concentration of the hydrophobic haptenic analyte in the unknown sample. A second assay method is conducted on the unknown sample to obtain a concentration of the interfering substance in the unknown sample. A predetermined correction formula that utilizes the measured concentration of the hydrophobic haptenic analyte and the measured concentration of the interfering substance obtained in step (a) is applied to determine an actual concentration of the hydrophobic haptenic analyte in the unknown sample.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: January 29, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Izak Bahar, Tie Q. Wei
  • Quantum dot-based optical sensors for rapid detection and quantitative analysis of biomolecules and biological materials
    Patent number: 10184935
    Abstract: The invention generally relates to detection and analysis of biological materials. In particular; the invention relates to quantum dot-based optical, sensors and methods for rapid detection and quantitative analysis of various biomolecules and biological materials, such as nucleic acids, proteins, cells, etc.
    Type: Grant
    Filed: April 5, 2011
    Date of Patent: January 22, 2019
    Assignee: University of Massachusetts
    Inventors: Triantafillos John Mountziaris, Jun Wang
  • Grafted dinuclear metal complexes, and use thereof as cellular microparticle sensors
    Patent number: 10180427
    Abstract: Novel grafted dinuclear metal complexes having formula (I) or of formula (II) wherein M represents a metal ion, and also the use thereof as sensors in a method for detecting and/or characterising cellular microparticles.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: January 15, 2019
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), UNIVERSITE DE CAEN BASSE-NORMANDIE, UNIVERSITE GRENOBLE ALPES
    Inventors: Catherine Belle, Gisele Gellon, Laurent Plawinski, Loic Doeuvre, Eduardo Angles Cano
  • Enhancement solution for enhancing chemiluminescence and method for preparing chemiluminescent solution
    Patent number: 10180424
    Abstract: Disclosed are an enhancement solution for enhancing chemiluminescence, and a method for preparing a chemiluminescent solution. The method comprises Step A: dissolving methylglucamine in water, adjusting the pH to 8-9, then adding an N-alkyldimethylmannosamine quaternary ammonium salt, acridine orange, BSA, glycine, MgCl2, and ZnCl2 respectively to a diethanolamine solution, stirring to dissolve them, and diluting to a constant volume, to obtain an enhancement solution; and Step B: diluting a chemiluminescent substrate with the enhancement solution, to obtain a chemiluminescent solution. When used in the field of immunoassays, the enhancement solution of the present invention increases the sensitivity and linear range of detection, reduces the cost, and is free of contamination.
    Type: Grant
    Filed: August 25, 2016
    Date of Patent: January 15, 2019
    Assignee: SHENZHEN MAXCHEMTECH CO., LTD.
    Inventors: Qi Ren, Jian Li
  • Rapid and sensitive method for detection of biological targets
    Patent number: 10168335
    Abstract: The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein ‘cross-linker’) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.
    Type: Grant
    Filed: May 26, 2016
    Date of Patent: January 1, 2019
    Assignee: DAKO DENMARK A/S
    Inventors: Jesper Lohse, Kenneth Heesche Petersen