Abstract: Disclosed are 1,4-dihydropyridine immunogen conjugates of immunogenic carrier materials coupled to a 1,4-dihydropyridine derivative. Said conjugates are useful for the preparation of antibodies thereto which antibodies may be used in immunoassays for 1,4-dihydropyridine compounds. An affinity column useful for the purification of said antibodies is also disclosed.

Abstract: A simple method for preparing highly radioactive 1,4-dihydropyridine and 1,4-dihydroquinoline derivatives labelled with .sup.125 I enables a specific radioactivity of approx. 2200 to 8800 Ci/m Mole to be achieved. In this way high sensitivity in medicament screening with the aid of radioreceptor assays and in determining plasma levels of 1,4-dihydropyridines, 1,4-dihydroquinolines and other substances that inter-react with receptors for 1,4-dihydropyridines and 1,4-dihydroquinolines is achieved, with the result that a substantially shorter exposure time in the auto-radiographic identification of the receptors for these substances is required. For this purpose an amino derivative of a 1,4-dihydropyridine or 1,4-dihydroquinoline is reacted with an acylating reagent radiactively labelled with .sup.125 I (2200-4400 Ci/mMole), and the .sup.

Abstract: Hybrid cell line for production of monoclonal antibody to an antigen found on approximately 95% of normal human thymocytes. The hybrid is formed by fusing splenocytes from immunized CAF.sub.1 mice with P3X63Ag8U1 myeloma cells. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.

Abstract: An antibody reactive with activated human platelets, and substantially unreactive with resting human platelets, with the azurophilic granules of monocytes, and with granulocytes.

Abstract: A mammalian monoclonal receptor is produced by a hybridoma formed by fusion of cells from a myeloma cell line and lymphocytes that produce antibodies that react with a viral antigen from a mammal immunized with cytomegalovirus-infected cells. The monoclonal receptor reacts with the viral antigen in a diagnostic system to detect the presence of cytomegalovirus in a biological sample.

Abstract: An immunoassay in which a thermally induced phase separation is used to effect the separation of specifically bound reactants from free reactants is disclosed. A first reactant is conjugated to a temperature-sensitive polymer to form a polymer/reactant conjugate, and a second reactant is conjugated to a reporter to form a reporter/reactant conjugate. The polymer/reactant, reporter/reactant, and biological fluid samples suspected of containing the analyte are admixed in solution at a temperature other than that at which the polymer will precipitate. Specific binding is allowed to occur, thereby forming a ternary complex. The salt concentration of the adjusted solution is then adjusted to a concentration sufficient to cause the complex to precipitate from the solution, the amount of reporter activity in the precipitated complex or in the solution measured and the presence and/or concentration of the analyte therefrom determined.

Abstract: Improved heterogeneous fluorescence assays are provided which employ solid supports comprising controlled pore glass particles which are substantially transparent at both the wavelength used to excite the fluorescent probe used in the assay and at the emission wavelength of the probe. The assays achieve sensitivities comparable to those achieved with radioimmunoassays without the need to concentrate the particles prior to the fluorescent measurement as in prior art heterogeneous fluorescence assays employing other types of solid supports.

Abstract: A method for use in the assay of PIVKA-II by the enzyme immunoassay using the two antibody-sandwiching method, characterized by using a monoclonal anti-PIVKA-II antibody as solidified antibody for said assay.

Abstract: Dyed inorganic composite particles having a mean particle diameter of 0.1 to 10.0 micrometers and a particle dispersibility value of at least 80%, each of said particles consisting of at least three layers comprising a core, a dyed layer on the surface of the core and a coated layer on the dyed layer, the core being composed of an inorganic compound, the dyed layer being composed of a dye or a mixture of a dye and an inorganic compound, and the coated layer being water-insoluble and light-pervious and composed of an inorganic compound or a mixture of it with a dye. The particles are useful as a carrier for preparing an immunological diagonostic reagent by binding an immunologically active substance (an antigen and an antibody) to them, particularly an immunological diagnostic reagent for use in a microtiter method, or as a hydrophilic pigment.

Abstract: A diagnostic test is described for the assay of human and animal immune system response, in vitro. The test measures production in a peripheral blood lymphocyte population of a material that is an index of mitogen-induced immune response. Adaptations of the test are provided for the titration of amplifier dosage, and for a determination whether amplifier treatment is warranted for immunodeficient patients such as those with AIDS or ARC.

Abstract: A method of inactivating viruses comprises bringing the viruses into contact with an effective amount of a photosensitizing agent which will bind to the viruses and/or virus infected cells, and then exposing the resulting mixture to visible light to excite and inactivate the viruses.

Abstract: The unit consists of a cuvette and light guiding means comporting more than one waveguide element in contact with the solution to be analyzed. During operation, signals issuing from said waveguide elements provide data on two or more parameters in the solution substantially simultaneously.

Abstract: Methods and compositions are provided for identifying patients suffering from neoplastic diseases such as breast cancer. It has been found that neoplastic epithelial cells, including neoplastic mammary epithelial cells, release a particular N-terminal blocked, soluble cytokeratin into circulation. The presence of this cytokeratin is diagnostic of neoplastic disease.

Abstract: Dry chemistry reagent system, kit and method for detection of an analyte such as glucose, cholesterol, urea, antigen or antibody. The reagent system is a porous membrane or bibulous film having a porosity gradient from one planar surface to the other. In the membrane are uniformly distributed an indicator, a flow control agent and a reagent cocktail. The membrane is also modified by a conditioning agent.

Abstract: A heterogeneous specific binding assay method for determining the amount of a suspected analyte in an aqueous test medium wherein a reaction mixture is formed by combining the test medium with assay reagents including a labeled reagent, an immobilizable component, and a binding substance which causes the immobilizable component to precipitate. Free and bound species of the labeled reagent are formed as a function of the amount of the analyte in the test medium. One of the free and bound species of the labeled reagent is immobilized by binding of the immobilizable component with the binding substance. The immobilized labeled reagent is seperated from labeled reagent which has not been immobilized, and the amount of label in the labeled reagent in one of the separated fractions is determined and related to the amount of analyte in the test medium.

Abstract: Polymorphisms in genes related to lipid metabolism, specifically apolipoproteins B, CII, E, and apoAIV, have been identified. Presence or absence of these polymorphisms in particular individuals may be correlated with propensity to show symptoms of atherosclerosis. Also thus correlated are two insertion polymorphisms 5' of the insulin gene.

Abstract: It has been found that immunologically related piliated organisms exhibit a hierarchic relationship wherein the cross-reactivity of the antisera to first pili against second pill in the series is greater for the senior members of the series than the cross-reactivity of the first pili with the corresponding antisera from second pili. This asymmetric relationship leads to the situation where the juniormost pili in the series may be utilized to detect the presence of all members of the family senior to it and conversely the seniormost pili may be utilized to protect against infection by all strains junior in the series.

Abstract: Fragments of human Factor VIII:C which bind to antibody inhibitors of Factor VIII in patients afflicted with such inhibitors are disclosed. The method of treating Factor VIII inhibitors, by administering one or more of these fragments, is also disclosed.

Abstract: Nucleic acid hybridization probes are provided which have sequences complementary to sequences of segments in bovine male-specific DNA and are suitable for sexing bovine embryos at the time of embryo transfer with virtually 100% accuracy.

Abstract: A carrier which consists of a carboxyl group-containing polymer, preferably activated polymethyl methacrylate, and on the surface of which a biologically active substance consisting of Poly I:C is immobilized, is described, as is a method of preparing such a carrier, and its use for diagnostic purposes. In the method of immobilizing Poly I:C on a carrier surface of polymethyl methacrylate, the surface of the polymethyl methacrylate carrier is first activated by treating it with strong alkali, such as a 10M sodium hydroxide solution. An aqueous solution of Poly I:C is heated, preferably for 1-2 h, and is then allowed to cool to about 50.degree.-80.degree. C., whereupon a coupler, preferably a water-soluble carbodiimide, is added to the solution. The warm solution is then applied to the activated carrier surface to immobilize Poly I:C on the carrier surface which then is allowed to cool to 0.degree.-50.degree. C.