Abstract: Recombinant DNA molecules are disclosed which contain a nucleotide sequence encoding a mouse cytomegalovirus-derived transcriptional promoter. Eucaryotic host cells transformed with recombinant DNA molecules having such mouse cytomegalovirus-derived promoter sequences operatively linked to heterologous protein-coding sequences may be cultured as a method for producing the heterologous proteins or as a method for identifying and isolating DNA encoding the heterologous protein via any method involving detection of the protein.

Abstract: Methods and compositions are provided for efficient expression of genes in unicellular microorganisms, particularly yeast. The systems involve an expression system employing transcriptional initiation regions from glycolytic enzymes, particularly a chimeric expression system, having a first region providing for regulatable or constitutive expression, a second region providing for transcriptional initiation, where regions one and two are not found joined together in functional relationship in nature, and optionally a sequence providing for a secretory leader and processing signal, where the expression cassette will be joined to a gene which may be homologous or heterologous to the host. The expression cassette can be used on an extrachromosomal element or integrated into the host genome, whereby continuous expression can be achieved or inducible expression is obtained, by virtue of the presence or absence of an inducer.

Abstract: The present invention is directed to recombinant baculoviruses which encode fusion polyhedrin proteins capable of forming occlusion bodies containing foreign peptides. The recombinant baculoviruses of the invention are formed by insertion into or replacement of regions of the polyhedrin gene that are not essential for occlusion body formation, with foreign DNA fragments by recombinant DNA techniques. The recombinant occlusion bodies produced in accordance with the present invention have uses in vaccine formulations, immunoassays, immobilized enzyme reactions, as biological insecticides, and as expression vectors.

Abstract: A new cloning system is described capable of expressing genetic material derived from recombinant DNA material, which comprises a yeast of the genus Kluyveromyces as a host. Suitable vectors are e.g. vectors containing autonomously replicating sequences (ARS) and vectors containing homologous Kluyveromyces DNA acting as a site for recombination with the host chromosome. New and preferred vectors are those containing ARS sequences originating from Kluyveromyces (KARS vectors). The genetically engineered new strains of Kluyveromyces produce, inter alia, lactase and chymosin.

Abstract: A method is provided for transferring genes into cells which comprises the step of subjecting the mixture of genes to be transferred and the target cells to an electric treatment.

Abstract: This invention relates to a DNA base sequence, capable of increasing the amount of protein secreted by microorganisms, and its derivative sequences; a recombinant plasmid including the whole or a part of said DNA base sequence; a method for preparing the recombinant plasmid in which, when microorganisms having introduced thereinto a recombinant DNA including the desired DNA base sequence are to be separated in the process of cloning, suitable transformants can be efficiently selected by taking the amount of protein secreted out of the cells and particularly the activity of an enzyme protein as an index; and a method of microbial breeding which comprises introducing the recombinant plasmid into a microorganism to increase the amount of protein secreted by the microorganism.

Abstract: Cloning and expression vector of a heterologous gene in a yeast, characterized in that it comprises at least; all or part of DNA of the plasmide k.sub.1 of Kluyveromyces lactis, a DNA segment incorporating the heterologous gene as well as the sequences providing the expression of said gene in said yeast.

Abstract: Biologically active PDGF analogs expressed in eucaryotic cells are disclosed. The analogs are produced by yeast strains transformed with an extrachromosomal element composed of a strong transcriptional promoter directing the expression of a gene which encodes a protein having substantially the same biological activity as PDGF. Suitable genes include the v-sis gene or a derivative of the v-sis gene of simian sarcoma virus or portions thereof, or the human cDNA gene for PDGF or portions thereof. In particular, DNA sequences encoding polypeptides substantially homologous to the B chain of PDGF are preferred. A secretory signal sequence may be provided upstream of the gene, enabling secretion of the gene product from the host cell. Mitogenic activity is one of the biological activities possessed by these PDGF analogs, making them useful in promoting the growth of mammalian cells.

Abstract: The present invention relates to a vector comprising part of AAV DNA contained in a plasmid and capable of being packaged into AAV particles and functioning as a vector for stable integration and expression of a gene in eukaryotic cells when under control of an AAV transcription promoter. A method of preparing such plasmids which are packagable and rescuable is also described.

Abstract: Methods and compositions are provided for the cloning and expression of Streptococcus pyogenes M protein genes, and, in particular, types 5, 6 and 24 genes in single-cell host organisms. The streptococcal M protein produced by the recombinant DNA techniques described herein may be formulated for use as immunogens in vaccines to protect against S. pyogenes infections. The gene for the M protein may further be employed as a molecular probe for the accurate identification of streptococci in infected body tissues and fluids.

Abstract: Methods for expressing a variety of biologically active PDGF analogs in eucaryotic cells are disclosed. The methods generally comprise introducing into a eucaryotic host cell a DNA construct capable of directing the expression and secretion of biologically active PDGF analogs in eucaryotic cells. The DNA construct contains a transcriptional promoter followed downstream by a suitable DNA sequence. The DNA sequence may encode a protein substantially homologous to the A-chain or the B-chain of PDGF, or a portion thereof, or an A-B heterodimer. In addition, a portion of the DNA sequence may encode at least a portion of the A-chain, while another portion encodes at least a portion of the B-chain of PDGF. Eucaryotic cells transformed with these DNA constructs are also disclosed. Methods of promoting the growth of mammalian cells, comprising incubating the cells with a biologically active PDGF analog expressed by a eucaryotic host cell transformed with such a DNA construct, are also disclosed.

Abstract: Insulin receptor is purified in accordance with this invention to a level sufficient to enable amino acid sequencing thereof. DNA encoding insulin receptor is provided, as well as methods for synthesizing insulin receptor or its mutant in heterologous host cells transformed with vectors containing such DNA. Knowledge of the amino acid sequence for insulin receptor enables the preparation of novel immunogenic conjugates and antibodies raised against such conjugates. Novel therapeutically useful forms of the insulin receptor and anti-receptor antibodies are described.

Abstract: Plasmids which are in themselves unstably inherited or which have become unstable due to the insertion of a DNA fragment comprising one or more genes not naturally related to the plasmid are stablized by means of a partitioning function exerted by a par region, especially a plasmid R1 par region, inserted into the plasmid on a DNA fragment which may be the length of the wild-type R1 EcoR1-. A fragment, but which is preferably shorter than this fragment, and which may comprise the R1 par region A, the R1 par region B or both these R1 par regions. The stabilization obtained for several different types of plasmid, especially by employing both R1 par regions, approaches the stability level of wild-type plasmids, i.e. they typically have a frequency of loss of less than 5.times.10.sup.31 6 per cell per generation.

Abstract: The present invention relates to pseudorabies virus mutants containing deletion and/or insertion mutations in a major viral glycoprotein gene, such that no antigenic polypeptides encoded by the viral gene are produced. As a result, animals vaccinated with such do not develop antibodies to the viral glycoprotein and can be distinguished from animals infected with pseudorabies virus field strains and known pseudorabies virus vaccine strains. The present invention also relates to vaccines for pseudorabies disease containing the same, methods for production of the same and methods for use of the same.

Abstract: Plant cell lines with altered flower structures, and altered plant male and/or female sterility/fertility, and altered polyamine synthesis have been isolated using a UV mutagenizing light, polyamine synthesis inhibitors, and growth in the dark. Some of these cell lines are resistant to MGBG and exhibit elevated polyamine synthesis. Regenerated plants with unusual flowers are thus possible as are crop and flower plants with unusual flowers, and/or altered fertility capability by the method of the invention. These plant lines are also useful as research tools.

Abstract: Disclosed are a novel method for inducing the high expression of a nucleotide sequence which is under the transcriptional and translational control of the yeast YG100 gene and the novel vectors, transformants and selectable DNA for the practice thereof.

Abstract: A method for the purification of a culture filtrate resulting from the fermentation of an organism of the species Mucor miehei involves the selective adsorption of the microbial rennet present in the culture filtrate by a blue dye affinity ligand with subsequent elution of the adsorbed microbial rennet to provide it in a purified form.

Abstract: L-Glutamic acid is produced in a high yield by cultivating an L-glutamic acid-producing microorganism which requires oleic acid but does not require biotin for growth in a culture medium containing an oleic acid compound and a biotin compound of no less than 100 .mu.g/liter as biotin, with carbohydrate and acetic acid as carbon sources being maintained in a weight ratio of about 80:20 through about 40:60.

Abstract: A c-Kirsten ras oncogene has been isolated from a human lung tumor cell line. This c-Kirsten ras has a mutation in codon 61 of the second coding exon and is capable of transforming NIH/3T3 mouse fibroblast cells to tumorigenic cells.

Abstract: A cDNA sequence encoding porcine beta FSH.