Abstract: Nucleic acid expression constructs are provided and, more particularly, nucleic acid constructs for expression of human alpha-galactosidase in plant cells, cells expressing the nucleic acid construct, producing the human alpha-galactosidase and uses thereof.

Abstract: Protein replacement therapy for patients with hemophilia or other inherited protein deficiencies is often complicated by pathogenic antibody responses, including antibodies that neutralize the therapeutic protein or that predispose to potentially life-threatening anaphylactic reactions by formation of IgE. Using murine hemophilia B as a model, we have developed a prophylactic protocol against such responses that is non-invasive and does not include immune suppression or genetic manipulation of the patient's cells. Oral delivery of coagulation factor IX (F. IX) expressed in chloroplasts, bioencapsulated in plant cells, effectively blocked formation of inhibitory antibodies in protein replacement therapy. Inhibitor titers were mostly undetectable and up to 100-fold lower in treated mice when compared to controls. Moreover, this treatment eliminated fatal anaphylactic reactions that occurred after 4 to 6 exposures to intravenous F. IX protein. While only 20-25% of control animals survived after 6-8 F.

Abstract: The present invention relates to a Lactuca sativa seed designated 81-64 RZ, which exhibits a combination of traits including resistance to downy mildew (Bremia lactucae) races Bl:1 to Bl:28, Bl:30-Bl:32 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri) and resistance to corky root (Sphingomonas suberifaciens) race CA1, as well as having non-flabellate leaves. The present invention also relates to a Lactuca sativa plant produced by growing the 81-64 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 81-64 RZ.

Abstract: Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated, target-specific RNA is provided by transcription of a chimeric gene comprising a promoter and a DNA region encoding the target-specific RNA.

Abstract: Compositions and methods for producing plant-derived immunogenic compositions that confer immunity against smallpox in a subject are provided. Plants engineered to produce antigenic proteins as well as expression cassettes comprising nucleic acids encoding proteins thereof are described. Methods of immunizing subjects with plant-derived compositions are also provided.

Abstract: Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi.

Abstract: Method for heterologous RNA species and protein production in plant cell mitochondria comprising introducing into plant cells nucleic acid components that encode heterologous proteins/RNAS under the control of promoters operative in mitochondria, vectors, host cells, plants and uses thereof.

Abstract: Polypeptide sequences for inducing recombinant protein bodies are described. The sequences comprise a polyproline II (PPII) structure and/or a proline-rich sequence between two cysteine residues on either end. Recombinant protein bodies are useful for protein production because they allow for simple and efficient purification of high quantities of recombinant protein. In addition, other methods of using recombinant protein bodies, for example, in vaccination and food products, are also described.

Abstract: The invention relates to the soybean variety designated 01057518. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01057518. Also provided by the invention are tissue cultures of the soybean variety 01057518 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01057518 with itself or another soybean variety and plants produced by such methods.

Abstract: The present invention is in the field of soybean variety SJ1312528 breeding and development. The present invention particularly relates to the soybean variety SJ1312528 and its seed, cells, germplasm, plant parts, and progeny, and methods of using SJ1312528 in a breeding program.

Abstract: The present invention relates to a Lactuca sativa seed designated 41-191 RZ, which exhibits a combination of traits including resistance to downy mildew (Bremia lactucae) races Bl:1 to Bl:32 and CA-I to CA-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, as well as having a hollow main vein, broad base leaves, a small head size and a small plant size. The present invention also relates to a Lactuca sativa plant produced by growing the 41-191 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 41-191 RZ.

Abstract: A soybean cultivar designated 56052100 is disclosed. The invention relates to the seeds of soybean cultivar 56052100, to the plants of soybean cultivar 56052100, to the plant parts of soybean cultivar 56052100, and to methods for producing progeny of soybean cultivar 56052100. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 56052100. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 56052100, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 56052100 with another soybean cultivar.

Abstract: The present invention relates to a Lactuca sativa seed designated 45-189 RZ, which may exhibit a combination of traits including resistance to downy mildew (Bremia lactucae) races Bl:1 to Bl:31 and CA-I to CA-VIII, currant lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, root aphids (Pemphigus bursarius), as well as having a medium head size, large outer leaves, and moderately glossy leaves. The present invention also relates to a Lactuca sativa plant produced by growing the 45-189 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 45-189 RZ.

Abstract: New lettuce varieties designated ‘Ezrilla’, ‘Analora’, and ‘Anandra’ are described. ‘Ezrilla’, ‘Analora’, and ‘Anandra’ are lettuce varieties exhibiting stability and uniformity.

Abstract: Method for heterologous protein production in plant cell plastids comprising introducing into plant cells nucleic acid components that encode heterologous proteins under the control of promoters operative in plastids, vectors, host cells, plants and uses thereof.

Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of a novel influenza HA protein in plants and its purification The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding improved influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.

Abstract: In certain embodiments transgenic plants (e.g., transgenic tomatoes) are provided that comprise cells that express a peptide one or more domains of which comprise the amino acid sequence of an apolipoprotein or apolipoprotein mimetic peptide where the peptide has biological activity (e.g., lowers SAA, and/or increases paroxonase activity, and/or improves HDL inflammatory index, etc.) when the plant and/or the peptide is fed to a mammal.

Abstract: The invention relates to the soybean variety designated 01050742. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01050742. Also provided by the invention are tissue cultures of the soybean variety 01050742 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01050742 with itself or another soybean variety and plants produced by such methods.

Abstract: The invention relates to the soybean variety designated 01050668. Provided by the invention are the seeds, plants and derivatives of the soybean variety 01050668. Also provided by the invention are tissue cultures of the soybean variety 01050668 and the plants regenerated therefrom. Still further provided by the invention are methods for producing soybean plants by crossing the soybean variety 01050668 with itself or another soybean variety and plants produced by such methods.

Abstract: The present invention relates to a Lactuca sativa seed designated 83-02 RZ, which may exhibit a combination of traits including resistance to downy mildew (Bremia lactucae) races B1:1-31 and Ca-I to Ca-VIII, resistance to currant-lettuce aphid (Nasonovia ribisnigri), very intensely red deeply lobed leaves, and a loose to moderately firm head. The present invention also relates to a Lactuca sativa plant produced by growing the 83-02 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 83-02 RZ.