Patents Examined by Steven G. Walsh
  • Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
    Patent number: 5491086
    Abstract: Recombinant DNA sequences encoding the DNA polymerase activity of Pyrodictium species can be used to construct recombinant vectors and transformed host cells for production of the activity. Pyrodictium enzymes for catalyzing 3'.fwdarw.5' exonuclease activity, i.e., proofreading enzymes, are also provided. The Pyrodictium enzymes are useful in DNA amplification procedures and are not irreversibly inactivated by exposure to 100.degree. C. in a polymerase chain reaction.
    Type: Grant
    Filed: May 14, 1993
    Date of Patent: February 13, 1996
    Assignee: Hoffmann-La Roche Inc.
    Inventors: David H. Gelfand, Alice M. Wang