Abstract: The disclosed device for trapping biologically-relevant substances can easily collect biologically-relevant substances from a thin tissue section or a gel in which said biologically-relevant substance have been fractionated. Said device is provided with: trapping bodies for trapping biologically-relevant substances; carriers that hold the trapping bodies; and electrodes for charging the trapping bodies. The trapping bodies are charged and brought into contact with a prescribed position on a thin tissue section that contains biologically-relevant substances or a gel in which biologically-relevant substances have been fractionated, thereby trapping said biologically-relevant substances from said gel or thin tissue section.

Abstract: There is provided a sensor testing method including: applying at least one of a first voltage that obtains a response caused by a substance and a second voltage that either obtains no response or substantially no response caused by the substance across a first electrode and a second electrode of a sensor; measuring current flowing between the first electrode and the second electrode; and determining whether or not there is a defect present in the sensor based on a quantity related to an amount of change per specific period of time of a current measured when the first voltage and/or the second voltage have been applied.

Abstract: An aspect includes a low slope electrode device, including: at least one electrode; at least one container at least partially enclosing the at least one electrode and having at least one ion sensitive region; an external buffer container having at least one separating element that separates said at least one ion sensitive region into: a first ion sensitive area separating an internal buffer solution bathing the at least one electrode and an external sample solution; and a second ion sensitive area separating the internal buffer solution bathing the at least one electrode and an external buffer solution; wherein the at least one separating element, the first ion sensitive area, and the second ion sensitive area establish a charge flow circuit. Other aspects are described and claimed.

Abstract: Electromechanical sensors that employ Janus micro/nano-components and techniques for the fabrication thereof are provided. In one aspect, a method of fabricating an electromechanical sensor includes the following steps. A back gate is formed on a substrate. A gate dielectric is deposited over the back gate. An intermediate layer is formed on the back gate having a micro-fluidic channel formed therein. Top electrodes are formed above the micro-fluidic channel. One or more Janus components are placed in the micro-fluidic channel, wherein each of the Janus components has a first portion having an electrically conductive material and a second portion having an electrically insulating material. The micro-fluidic channel is filled with a fluid. The electrically insulating material has a negative surface charge at a pH of the fluid and an isoelectric point at a pH less than the pH of the fluid.

Abstract: Electromechanical sensors that employ Janus micro/nano-components and techniques for the fabrication thereof are provided. In one aspect, a method of fabricating an electromechanical sensor includes the following steps. A back gate is formed on a substrate. A gate dielectric is deposited over the back gate. An intermediate layer is formed on the back gate having a micro-fluidic channel formed therein. Top electrodes are formed above the micro-fluidic channel. One or more Janus components are placed in the micro-fluidic channel, wherein each of the Janus components has a first portion having an electrically conductive material and a second portion having an electrically insulating material. The micro-fluidic channel is filled with a fluid. The electrically insulating material has a negative surface charge at a pH of the fluid and an isoelectric point at a pH less than the pH of the fluid.

Abstract: A sheath-flow interface for producing electrospray from a capillary is provided. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. In the interface, electrokinetic flow moves a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The mixture of sheath liquid and analyte is directed to an electrospray emitter in order to generate an electrospray.

Abstract: A biosensor system including the underfill management system determines the analyte concentration in a sample from the at least one analytic output signal value. The underfill management system includes an underfill recognition system and an underfill compensation system. The underfill recognition system determines whether the test sensor initially is substantially full-filled or underfilled, indicates when the sample volume is underfilled so that additional sample may be added to the test sensor, and starts or stops the sample analysis in response to the sample volume. The underfill recognition system also may determine the initial degree of underfill. After the underfill recognition system determines the initial fill state of the test sensor, the underfill compensation system compensates the analysis based on the initial fill state of the test sensor to improve the measurement performance of the biosensor system for initially underfilled test sensors.

Abstract: The invention relates transiently attaching drag-tags to molecules during electrophoresis. The invention includes running buffers having drag-tags that transiently attach to lipophilic moieties attached to the molecules. The lipophilic moieties can be covalently or ionically bonded to the molecules. One particular aspect of the invention is a nucleoside analog or a nucleic acid analog comprising a lipophilic moiety. The invention is also directed to methods of separating molecules that comprise a lipophilic moiety. The methods generally comprise transiently attaching a drag-tag to the lipophilic moiety during a separation modality. These methods can be used to separate the molecules by size or weight, to measure a hydrodynamic radius of a drag-tag, or to separate a plurality of drag-tag by their hydrodynamic radius.

Abstract: Compositions containing vinyl polymers and ionophores selective for lead ions (e.g., aniline copolymers), and methods for making these compositions are disclosed herein. The compositions can, for example, be used for detecting lead ions in a sample.

Abstract: The present disclosure provides an apparatus for sample introduction including a flow channel, a sample inlet, a metering unit, a separating unit, cell dischargers and, and a pressure controlling unit. The sample inlet is given a sample fluid such as blood. The sample inlet has a constricting hole that communicates with the flow channel for passage of a carrier fluid. The constricting hole has a diameter large enough for passage of a single cell contained in the sample fluid. The sample fluid is introduced into the flow channel through the constricting hole, and the cells in the sample fluid pass one by one through the constricting hole. The constricting hole does not exist inside the flow channel for passage of the carrier fluid, so that the diameter of the constricting hole does not affect the flow rate of the carrier fluid passing through the flow channel.

Abstract: An electrochemical ethylene sensor and method for ethylene sensing are disclosed. In one aspect, an electrochemical ethylene sensor includes a working electrode and a counter electrode on an electrically insulating substrate. An ionic liquid layer covers the working electrode and counter electrode. In one method, a voltage is applied to the working electrode which is equal to or lower than the voltage required for the onset of oxidation of the material of the working electrode, for example, in the range spanning 700 mV before the onset of oxidation of the material of the working electrode.

Abstract: In a method and apparatus for determining information concerning the presence of ingredients with oxygen demand in a liquid sample, especially for determining the chemical oxygen demand of a liquid sample, especially a water, or wastewater, sample, by means of electrochemical oxidation of ingredients of the liquid sample, oxidation of ingredients of the liquid sample occurs on a boron doped, diamond electrode.

Abstract: Systems and methods are provided for producing fluids with desired concentration factors. According to one embodiment, an arrangement of digital microfluidic (DMF) based electrode platforms are provided. The arrangement may be configured to carry out a sequence of mix steps that may demand storage of resultant fluid mixtures produced in intermediate mix steps. Such sequences of mix steps may be desirable as a result of the decreased demand for initial fluid samples, and reduced wastage.

Abstract: A sensing apparatus for sensing target materials including biological or chemical molecules in a fluid. One such apparatus includes a semiconductor-on-insulator (SOI) structure having an electrically-insulating layer, a fluidic channel supported by the SOI structure and configured and arranged to receive and pass a fluid including the target materials, and a semiconductor device including at least three electrically-contiguous semiconductor regions doped to exhibit a common polarity. The semiconductor regions include a sandwiched region sandwiched between two of the other semiconductor regions, and configured and arranged adjacent to the fluidic channel with a surface directed toward the fluidic channel for coupling to the target materials in the fluidic channel, and further arranged for responding to a bias voltage. The sensing apparatus also includes an amplification circuit in or on the SOI and that is arranged to facilitate sensing of the target material near the fluidic channel.

Abstract: Electrochemiluminescent technique and device suitable for cheap analytical and diagnostic applications, with electrodes manufactured from carbon paste and terbium chelates as labeling compounds.

Abstract: An active matrix electrowetting on dielectric (AM-EWOD) device includes a substrate electrode and a plurality of array elements, each array element including an array element electrode. The AM-EWOD device further includes thin film electronics disposed on a substrate. The thin film electronics includes first circuitry configured to supply a first time varying signal V1 to the array element electrodes, and second circuitry configured to supply a second time varying signal V2 to the substrate electrode. An actuation voltage is defined by a potential difference between V2 and V1, and the first circuitry further is configured to adjust the amplitude of V1 to adjust the actuation voltage. V1 may be adjusted to adjust the actuation voltage while V2 remains unchanged. The actuation voltage may be controlled to operate the AM-EWOD device between high and low voltage modes of operation in accordance with different droplet manipulation operations to be performed.

Abstract: Methods for distinguishing between an aqueous non-blood sample (e.g., a control solution) and a blood sample. In one aspect, the methods include using a test strip in which multiple current transients are measured by a meter electrically connected to an electrochemical test strip. The current transients are used to determine if a sample is a blood sample or an aqueous non-blood sample based on at least two characteristics (e.g., amount of interferent present and reaction kinetics). The method can also include calculating a discrimination criteria based upon at least two characteristics. Various aspects of a system for distinguishing between blood samples and an aqueous non-blood sample are also provided herein.

Abstract: Method and a device for capturing heavy metal ions included in sewage sludge. The method includes steps of: a) placing in the fluid a functionalized radiografted track-etched membrane FRTEM which contains polymer nanopores; this membrane including a first electrode on one side of the membrane, b) selectively capturing heavy metal ions inside the polymer nanopores, c) applying an anodic stripping voltammetric ASV analysis on the membrane in order to differentiate and quantify captured metal ions, the first electrode being used as an ASV detection electrode.

Abstract: A nanopore sensor comprises second electrophoresis electrode or micropump, second fluidic reservoir, second micro-nanometer separation channel, substrate, sub-nanometer-thick functional layer, first micro-nanometer separation channel, first electrophoresis electrode or micropump, and first electrophoresis electrode or micropump that are sequentially assembled. An opening and a nanopore are provided through the substrate and the sub-nanometer-thick functional layer, respectively. A first electrode for measuring ionic current is provided in the first micro-nanometer separation channel, and a second electrode for measuring ionic current is provide in the second micro-nanometer separation channel. The present invention provides a simple method to prepare a sub-nanometer functional layer having a nanopore extending through the sub-nanometer-thick functional layer. The pore size is comparable to the spacing between two adjacent bases in a DNA strand required for single-base resolution sequencing.

Abstract: A method for isotope measurement of charged species contained in a solution to be analyzed, particularly charged species having an isobaric interference, has the following consecutive steps: a) in the capillary of a capillary electrophoresis device, the solution to be analyzed is inserted contiguously between a terminating electrolyte and a leading electrolyte that, respectively, are placed after the inlet and before the outlet of the capillary and contain ions of the same charge but with mobility inferior and superior to those of said species; b) separating the species by using the capillary electrophoresis device according to the isotachophoresis mode; then c) in the continuity of the preceding step, performing an isotope measurement of the species detected in the form of a substantially constant amplitude signal by using an inductively coupled plasma mass spectrometer (ICPMS) connected by direct coupling with the capillary electrophoresis device.