Patents Examined by Suzanne M. Noakes
  • Patent number: 11225675
    Abstract: The present invention provides D-lactate dehydrogenase, an engineered strain containing the D-lactate dehydrogenase and a construction method and use of the engineered strain. The present invention discloses a D-lactate dehydrogenase which has unique properties and is from Thermodesulfatator indicus, and the D-lactate dehydrogenase has good thermophily and heat stability. By using the D-lactate dehydrogenase and the gene engineering reconstruction method, a fermentation product of the reconstructed Bacillus licheniformis can be redirected to optically-pure D-lactic acid with a high yield from naturally produced 2,3-butanediol, and the optical purity of the produced D-lactic acid reaches 99.9%; and raw materials for fermentation are low-cost, and a fermentation state is between an anaerobic fermentation state and a microaerobic fermentation state.
    Type: Grant
    Filed: June 24, 2020
    Date of Patent: January 18, 2022
    Assignee: Shanghai Jiao Tong University
    Inventors: Ping Xu, Chao Li, Fei Tao, Hongzhi Tang
  • Patent number: 11224641
    Abstract: The invention provides compositions comprising Eno1 for delivery to a muscle. Further, the invention provides a method for normalizing blood glucose in a subject with elevated blood glucose, comprising administering to the subject enolase 1 (Eno1), thereby normalizing blood glucose in the subject. The invention also provides methods of treating one or more conditions including impaired glucose tolerance, insulin resistance, pre-diabetes, and diabetes, especially type 2 diabetes in a subject, comprising administering to the subject enolase 1 (Eno1), thereby treating the condition in the subject. In certain methods of the invention, the Eno1 is delivered to muscle.
    Type: Grant
    Filed: November 28, 2018
    Date of Patent: January 18, 2022
    Assignee: Berg LLC
    Inventors: Niven Rajin Narain, Rangaprasad Sarangarajan, Vivek K. Vishnudas, Stephane Gesta, Enxuan Jing
  • Patent number: 11220677
    Abstract: Provided are: a reverse transcriptase mutant including an amino acid mutation at a position corresponding to position 55 of the amino acid sequence of wild-type reverse transcriptase derived from the Moloney murine leukemia virus, wherein the reverse transcriptase mutant is characterized in that the amino acid mutation is a substitution from threonine to another amino acid, and the other amino acid is selected from the group consisting of amino acids having a nonpolar aliphatic side chain and amino acids having a polar acidic functional group side chain; a nucleic acid that encodes the mutant; a method for producing the mutant and the nucleic acid that encodes the mutant; a method for synthesizing cDNA in which the mutant is used; and a composition and kit including the mutant.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: January 11, 2022
    Assignee: TAKARA BIO INC.
    Inventors: Kazuhiko Ishikawa, Takashi Uemori, Nariaki Takatsu
  • Patent number: 11220678
    Abstract: Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: January 11, 2022
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Benjamin Kleinstiver
  • Patent number: 11214588
    Abstract: This invention relates to a method for the enzymatic synthesis of oligosaccharides, preferably human milk oligosaccharides (HMOs) The method comprises the enzymatic transfer of a glycosyl moiety and subsequent removal of by-products, such as lactose, by nanofiltration using a membrane comprising an active polyamide layer.
    Type: Grant
    Filed: June 27, 2018
    Date of Patent: January 4, 2022
    Assignee: GLYCOM A/S
    Inventors: Nikolay Khanzhin, Markus Jondelius Hederos
  • Patent number: 11208700
    Abstract: Pharmaceutical compositions comprising spray-dried oxalate decarboxylase crystals are disclosed. Methods to treat a disorder associated with elevated oxalate concentration using compositions comprising spray-dried oxalate decarboxylase crystals are also disclosed.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: December 28, 2021
    Assignee: Allena Pharmaceuticals, Inc.
    Inventors: Jack Greene, Richard Johnson, Dennis Krushinskie, Bhami Shenoy
  • Patent number: 11208643
    Abstract: The present invention relates in general to the field of antimicrobial enzymes. In particular, the present invention relates to a polypeptide comprising the amino acid sequence of a globular Gram-negative endolysin and the amino acid sequence of a cell wall binding domain of i) a modular Gram-negative endolysin or ii) a bacteriophage tail/baseplate protein. The present invention relates also to corresponding nucleic acids, vectors, bacteriophages, host cells, and compositions. The present inventions also relates to the use of the polypeptide, nucleic acids, vectors, bacteriophages, host cells, and compositions in methods for treatment of the human or animal body by surgery or therapy or in diagnostic methods practiced on the human or animal body. The polypeptides, nucleic acids, vectors, bacteriophages, host cells, and compositions according to the invention may also be used as an antimicrobial in, e.g., food or feed, in cosmetics, or as disinfecting agent.
    Type: Grant
    Filed: April 2, 2018
    Date of Patent: December 28, 2021
    Assignee: SASINAPAS CO., LTD.
    Inventors: Martin Griessl, Manfred Biebl
  • Patent number: 11208639
    Abstract: The present invention relates to polypeptides having DNase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: March 23, 2018
    Date of Patent: December 28, 2021
    Assignee: Novozymes A/S
    Inventors: Mary Ann Stringer, Jesper Salomon, Klaus Gori, Jürgen Carsten Franz Knötzel
  • Patent number: 11208736
    Abstract: Provided herein are scalable methods of processing wet-spun fiber comprising recombinant spider silk polypeptides to generate a three-dimensional crystalline lattice of beta-sheet structures in the fiber.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: December 28, 2021
    Assignee: Bolt Threads, Inc.
    Inventors: Amir Ahmad Bakhtiary Davijani, Steven Tom, Maxime Boulet-Audet, Paul Andre Guerette, Lindsay Wray, Nicole Elizabeth Subler, Zoya Nasir Zulfiqar, Hua Li, Jessica Wang
  • Patent number: 11203764
    Abstract: The present invention relates a variant polypeptide having kaurenoic acid 13-hydroxylase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 72, 85, 108, 127, 129, 141, 172, 195, 196, 197, 199, 226, 236, 291, 302, 361 or 464, said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
    Type: Grant
    Filed: July 24, 2020
    Date of Patent: December 21, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Gustaaf Ernst Van Leeuwen, Priscilla Zwartjens, Viktor Marius Boer
  • Patent number: 11203760
    Abstract: The invention can be used in biotechnology, in particular to the gene therapy DNA vector GDTT1.8NAS12, Escherichia coli strain JM 110-NAS, Escherichia coli strain JM 110-NAS/GDTT1.8NAS12, and the industrial production of the gene therapy DNA vector GDTT1.8NAS12. A method of obtaining the gene therapeutic DNA vector GDTT1.8NAS12 involves construction of a 2408-bp intermediate vector containing a 688-bp replication origin, a 467-bp transcription terminator hGH-TA, a 137-bp regulatory site RNA-out transposon TnIO, a I0I8-bp kanamycin resistance gene, and a 68-bp polylinker. Then, it is splitted using restriction endonucleases SalI and BamHI and ligated to the promoter regulatory region containing the 1219-bp promoter region of the human elongation factor EF1A with its own enhancer. The kanamycin resistance gene is cleaved at the SpeI restriction sites.
    Type: Grant
    Filed: July 26, 2019
    Date of Patent: December 21, 2021
    Assignees: GENETIC DIAGNOSTICS AND THERAPY 21 LTD, Obschestvo s ogranichennoi otvetstvennostju «REKOMBITEKH»
    Inventor: Anton Gamolski
  • Patent number: 11203741
    Abstract: The invention relates to a recombinant cell, preferably a yeast cell comprising: a) one or more heterologous genes encoding a glycerol dehydrogenase activity; b) one or more genes encoding a dihydroxyacetone kinase (E.C. 2.7.1.28 and/or E.C. 2.7.1.29); c) one or more heterologous genes encoding a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39, RuBisCO); and d) one or more heterologous genes encoding a phosphoribulokinase (EC 2.7.1.19, PRK); and optionally e) one or more heterologous genes encoding for a glycerol transporter, wherein the recombinant yeast comprises overexpression of one or more PPP-genes. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.
    Type: Grant
    Filed: June 11, 2021
    Date of Patent: December 21, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz, Jozef Petrus Johannes Schmitz, Hans Marinus Charles Johannes De Bruijn
  • Patent number: 11198895
    Abstract: Fermentative production of L-lysine using an L-lysine excreting bacterium of the species Corynebacterium glutamicum having a completely or partly deleted whiB4 gene is provided.
    Type: Grant
    Filed: November 27, 2019
    Date of Patent: December 14, 2021
    Assignee: Evonik Operations GmbH
    Inventors: Georg Thierbach, Frank Schneider, Kornelia Voß, Thomas Bekel
  • Patent number: 11198896
    Abstract: One aspect of the invention provides a nucleic acid encoding a 7?-hydroxysteroid dehydrogenase (7?-HSDH) that catalyzes at least the stereospecific enzymatic reduction of a 7-ketosteroid to the corresponding 7-hydroxysteroid. The enzyme includes a mutation at position 64 of SEQ ID NO:2 or in the corresponding sequence positions of an amino acid sequence derived therefrom with at least 90% sequence identity to SEQ ID NO:2. The mutation at position 64 is the mutation R64X1, wherein X1 represents E, D, T, L, S, P, V, K, C, A, G, Q, F, W, I or Y. The enzyme shows the following property profile in comparison with the 7?-HSDH with SEQ ID NO:2: (a) an increased specific activity (Vmax [U/mg]) for NADPH in the enzymatic reduction of dehydrocholic acid (DHCA) with NADPH as cofactor.
    Type: Grant
    Filed: July 10, 2020
    Date of Patent: December 14, 2021
    Assignee: Pharmazell GmbH
    Inventors: Werner Hummel, Daniel Bakonyi
  • Patent number: 11198857
    Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.
    Type: Grant
    Filed: July 29, 2021
    Date of Patent: December 14, 2021
    Assignee: Inscripta, Inc.
    Inventors: Aamir Mir, Andrew Garst, Stephen Federowicz, Kyle Seamon
  • Patent number: 11198862
    Abstract: The present invention relates to a method for promoting acetylglucosamine synthesis of Bacillus subtilis, which belongs to the field of genetic engineering. The present invention adopts the recombinant Bacillus subtilis BSGNKAP2 as a starting strain, exogenously introducing pyruvate carboxylase BalpycA derived from Bacillus cereus, eliminating the central carbon metabolism overflow of the Bacillus subtilis and avoiding the synthesis of the by-product acetoin; further, five exogenous reducing force metabolic reactions are introduced to replace the reaction of generating NADH in glycolysis pathway and tricarboxylic acid cycle to reconstruct intracellular reducing force metabolism, which specifically comprise glyceraldehyde-3-phosphate ferredoxin dehydrogenase, isocitrate NAD+ dehydrogenase, a malate quinone dehydrogenase, a ketoacid ferredoxin oxidoreductase and a nitrogenase ferritin. In a shake-flask fermentation process using a complex medium, acetylglucosamine yield of the recombinant strain BSGNKAP8 is 24.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: December 14, 2021
    Assignee: Jiangnan University
    Inventors: Long Liu, Yang Gu, Jieying Deng, Jian Chen, Guocheng Du, Jianghua Li
  • Patent number: 11191816
    Abstract: Provided herein are compositions comprising mutant GADPH. Methods for treating or preventing cancer in a subject by administering to the subject a therapeutically effective amount of mutant GAPDH compositions are provided.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: December 7, 2021
    Assignee: The Johns Hopkins University
    Inventor: Shanmugasundaram Ganapathy-Kanniappan
  • Patent number: 11193150
    Abstract: The present disclosure provides isolated nepetalactone oxidoreductase polypeptides (NORs), nepetalactol synthases (NEPSs), and related polynucleotides, engineered host cells, and cultures, as well as methods for producing NORs and NEPSs, and for using them to produce nepetalactol, nepetalactone, and dihydronepetalactone. The present disclosure also provides methods for engineering cells (e.g., microbial cells) to produce nepetalactone from a fermentation substrate such as glucose, as well as engineered cells having this capability and related cultures and methods for producing nepetalactone.
    Type: Grant
    Filed: May 29, 2020
    Date of Patent: December 7, 2021
    Assignee: ZYMERGEN INC.
    Inventors: Grayson Wawrzyn, Christine Roche, Patrick J. Westfall, Warren Lau, Savita Ganesan, Fern R. McSorley, Zach Serber
  • Patent number: 11186853
    Abstract: The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid.
    Type: Grant
    Filed: November 13, 2019
    Date of Patent: November 30, 2021
    Assignee: Genomatica, Inc.
    Inventors: Robin E. Osterhout, Anthony P. Burgard
  • Patent number: 11180767
    Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.
    Type: Grant
    Filed: June 26, 2020
    Date of Patent: November 23, 2021
    Assignee: GLYKOS FINLAND OY
    Inventors: Christopher Landowski, Anne Huuskonen, Juhani Saarinen, Ann Westerholm-Parvinen, Anne Kanerva, Jari Natunen, Anna-Liisa Hänninen, Noora Salovuori, Merja Penttilä, Markku Saloheimo