Patents Examined by Teresa E Strzelecka
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Patent number: 11952620Abstract: According to one embodiment, a method of quantifying a target nucleic acid containing a first sequence in a sample is provided. The method includes preparing a substrate on which a plurality of detection regions are arranged, forming a reaction field by placing, on the substrate, a reaction liquid containing a sample, a primer set, and an amplification enzyme, maintaining the reaction field in an isothermal amplification condition, detecting a detection signal for each of the detection regions, determining, for each of the plurality of detection regions, whether positive or negative and detecting or quantifying the target nucleic acid based on the number of positive and/or a rise time of each of the positive detection signal.Type: GrantFiled: November 11, 2020Date of Patent: April 9, 2024Assignee: KABUSHIKI KAISHA TOSHIBAInventors: Koji Hashimoto, Keiko Ito, Mika Inada
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Patent number: 11932912Abstract: The present specification discloses a method for detecting an aneuploid of a Brassica oleracea plant, including: performing real-time PCR using DNA extracted from a sample derived from a Brassica oleracea plant to be tested as a template and DNA markers specific to each of two or more chromosomes of Brassica oleracea plant; and detecting chromosomal aneuploidy from a relative difference in amplification amount between the obtained DNA markers. According to one embodiment of the present invention, it is possible to simply, accurately, and rapidly detect an off-type (chromosomal aneuploid) that may occur in Brassica oleracea varieties, in a laboratory equipped with a general molecular biological equipment in the course of seed quality control and breeding research.Type: GrantFiled: August 17, 2018Date of Patent: March 19, 2024Assignee: SAKATA SEED CORPORATIONInventors: Atsushi Izumida, Takao Suzuki, Tetsuya Hiramoto
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Patent number: 11920188Abstract: Provided herein is a method for detecting the presence of clade variants in the COVID-19 virus in a human sample and/or an environmental sample. Samples are processed to obtain total RNA. The RNA is used as a template in a combined reverse transcription and amplification reaction to obtain fluorescent COVID-19 virus amplicons. These amplicons are hybridized on a microarray with nucleic acid probes having sequences that discriminate among the various clade variants. The microarray is imaged to detect the clade variant. Also provided is a method of distinguishing each clade variant from others by generating an intensity distribution profile from the image, which is unique to each of the clade variants.Type: GrantFiled: May 27, 2021Date of Patent: March 5, 2024Inventors: Frederick Henry Eggers, Benjamin Alan Katchman, Fushi Wen, Candy Mavis Rivas, Cory Scott Newland, Michael Edward Hogan
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Patent number: 11913067Abstract: A composition for amplifying a polynucleotide is provided that includes a substrate comprising a first region and a second region. A first plurality of capture primers is coupled to the first region of the substrate. A second plurality of capture primers is coupled to the second region of the substrate. The capture primers of the second plurality of capture primers are longer than the capture primers of the first plurality of capture primers. A first plurality of orthogonal capture primers are coupled to the first region of the substrate. A second plurality of orthogonal capture primers are coupled to the second region of the substrate. The orthogonal capture primers of the second plurality of orthogonal capture primers are shorter than the orthogonal capture primers of the first plurality of orthogonal capture primers.Type: GrantFiled: August 27, 2021Date of Patent: February 27, 2024Assignees: Illumina, Inc., Illumina Cambridge LimitedInventors: Jeffrey Fisher, Jason Betley
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Patent number: 11898202Abstract: The present invention disclosure relates to a next generation DNA sequencing method and use for accurate and massively parallel quantification of one or more nucleic acid targets, for example in large volumes of unpurified sample material. More particularly, the invention is related to a method and a kit comprising probes for detecting and quantifying genetic targets in complex samples. The invention includes one or more target-specific nucleic acid probes per genetic target and a bridge oligo or bridge oligo complex.Type: GrantFiled: March 3, 2022Date of Patent: February 13, 2024Assignee: Genomill Health OyInventors: Juha-Pekka Pursiheimo, Tatu Hirvonen, Manu Tamminen, Anttoni Korkiakoski
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Patent number: 11898210Abstract: The present invention relates to an in vitro method for identifying subjects hosting high amounts of Fim H expressing proteobacteria in their gut, said method comprising the step of detecting the expression of the fimH gene in a stool sample of said subjects.Type: GrantFiled: October 16, 2018Date of Patent: February 13, 2024Assignee: ENTEROMEInventors: Alessandra Cervino, Christophe Bonny, Jonathan Plassais
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Patent number: 11891658Abstract: The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). The methods described herein can be used to achieve clonal amplification even with a greater than Poisson distribution of beads and/or nucleic acid templates into an emulsion. A PCR method may comprise generating a partition (e.g., a droplet) comprising at least two beads and/or at least two nucleic acid molecules and generating clonal amplification products corresponding to the nucleic acid molecule, at least a subset of which may be attached to a bead.Type: GrantFiled: August 5, 2021Date of Patent: February 6, 2024Assignee: ULTIMA GENOMICS, INC.Inventors: Gilad Almogy, Florian Oberstrass, Omer Barad, Chandan Shee
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Patent number: 11891654Abstract: Provided herein are methods of determining a location of a target nucleic acid in a biological sample.Type: GrantFiled: February 24, 2021Date of Patent: February 6, 2024Assignee: 10x Genomics, Inc.Inventors: Luigi Jhon Alvarado Martinez, Eswar Prasad Ramachandran Iyer
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Patent number: 11884973Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.Type: GrantFiled: November 16, 2017Date of Patent: January 30, 2024Assignee: Hitachi, Ltd.Inventors: Junko Tanaka, Takahide Yokoi, Masao Kamahori, Yoshinobu Kohara
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Patent number: 11884972Abstract: In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.Type: GrantFiled: June 23, 2017Date of Patent: January 30, 2024Assignee: Carlsberg A/SInventors: Toni Wendt, Ole Olsen, Søren Knudsen, Hanne Cecille Thomsen, Birgitte Skadhauge, Magnus Wohlfahrt Rasmussen, Massimiliano Carciofi, Alexander Striebeck
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Patent number: 11873325Abstract: The present invention relates to nucleic acids encoding for proteins correlated with the resistance of the Sm1 locus in wheat and uses of these nucleic acids, in particular for conferring or improving resistance to orange wheat blossom midge (OWBM) in a plant.Type: GrantFiled: October 10, 2017Date of Patent: January 16, 2024Assignee: LIMAGRAIN EUROPEInventors: Simon Timothy Berry, Jordi Comadran, Sebastien Specel
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Patent number: 11866782Abstract: This disclosure provides methods and systems for single-cell, multi-omic analysis of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile. Nucleic-acid-tagged antibody conjugates are used for simultaneous proteomic analysis along with the gene expression profiling.Type: GrantFiled: March 16, 2021Date of Patent: January 9, 2024Assignee: Fluent Biosciences Inc.Inventor: Sepehr Kiani
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Patent number: 11857940Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.Type: GrantFiled: August 10, 2021Date of Patent: January 2, 2024Assignee: Fluidigm CorporationInventor: Peilin Chen
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Patent number: 11851720Abstract: Provided herein are methods for identifying the presence or absence of a target nucleic acid from a microorganism using direct amplification without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids.Type: GrantFiled: April 10, 2020Date of Patent: December 26, 2023Assignee: Quest Diagnostics Investments LLCInventors: Maurice Exner, Luca Jacky, Yin-Peng Chen, Huong Mai, Michelle M. Tabb, Michael Aye
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Patent number: 11851699Abstract: Disclosed are methods for isothermal nucleic acid amplification and detection.Type: GrantFiled: February 5, 2021Date of Patent: December 26, 2023Assignee: Board of Regents, The University of Texas SystemInventors: Andrew Ellington, Yu Sherry Jiang, Sanchita Bhadra, Bingling Li, Randy Allen Hughes, Yan Du, Jimmy Gollihar
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Patent number: 11851703Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.Type: GrantFiled: October 15, 2020Date of Patent: December 26, 2023Assignee: Life Technologies CorporationInventors: John Leamon, Mark Andersen, Michael Thornton
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Patent number: 11845924Abstract: Reagents and methods for preparing nucleic acid samples for sequencing are provided. The reagents include multimeric barcoding reagents that comprise barcode regions linked together and a cell-binding moiety. The methods comprise contacting a nucleic acid sample comprising cells with a library of multimeric barcoding reagents, wherein each multimeric barcoding reagent comprises barcode regions linked together, and appending barcode sequences of a first multimeric barcoding reagent to sub-sequences of a target nucleic acid of a first cell, and appending barcode sequences of a second multimeric barcoding reagent to sub-sequences of a target nucleic acid of a second cell. Methods are also provided that comprise steps of internalising multimeric barcoding reagents into cells (e.g. by endocytosis) or exposing multimeric barcoding reagents to target nucleic acids by lysing cells or permeabilizing cell membranes.Type: GrantFiled: March 17, 2023Date of Patent: December 19, 2023Assignee: CS Genetics LimitedInventor: Lucas Brandon Edelman
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Patent number: 11840716Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5? to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5? to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.Type: GrantFiled: December 28, 2020Date of Patent: December 12, 2023Assignee: GEN-PROBE INCORPORATEDInventors: Norman C. Nelson, Margarita Batranina-Kaminsky
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Methods for detection and quantification of infectious carbapenem resistant enterobacteriaceae (CRE)
Patent number: 11840723Abstract: Provided herein are qPCR-based methods for quantitatively detecting and differentiating between live and dead pathogenic bacteria such as carbapenem-resistant Enterobacteriaceae (CRE). Also provided herein are simple, fast, and reliable genomic DNA extraction methods with improved efficiency.Type: GrantFiled: June 11, 2021Date of Patent: December 12, 2023Assignees: Arizona Board of Regents on behalf of Arizona State University, Mayo Foundation for Medical Education and ResearchInventors: Joshua LaBaer, Vel Murugan, Merica Vorachitti, Rahul Pannala, Douglas Faigel -
Patent number: 11840721Abstract: The present disclosure generally relates to the field of microbial pathogen detection and identification utilizing genomic sequence recognition.Type: GrantFiled: April 2, 2018Date of Patent: December 12, 2023Assignee: HelixBind, Inc.Inventors: Alon Singer, Ranjit Prakash, Jork Nolling