Patents Examined by Teresa Strzelecka
-
Patent number: 8435743Abstract: The present invention provides a cotton plant event MON 88913 compositions and seed. Also provided are assays for detecting the presence of the cotton plant event MON 88913 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.Type: GrantFiled: November 4, 2011Date of Patent: May 7, 2013Assignee: Monsanto Technology LLCInventors: R. Eric Cerny, Can Duong, Jesse L. Hart, Scott A. Huber, Rachel L. Krieb, Jennifer J. Listello, Amy B. Martens, Bernard Sammons
-
Patent number: 8017318Abstract: The invention concerns a method for detecting a known function, from nucleic acids present in a sample, characterized in that it comprises the following steps: (a) preparing, from the nucleic acids of the sample, nucleic acid molecules comprising the gene(s) coding for the protein(s) corresponding to said function, and the control elements required for the transcription and the translation of said gene(s); (b) in vitro transcription and translation of the nucleic acid molecule prepared in step (a); (c) detecting and/or measuring the function corresponding to the protein(s) produced in step (b).Type: GrantFiled: November 28, 2000Date of Patent: September 13, 2011Assignee: Proteus S.A.Inventors: Jean François Bloch, Sandrine Dautel, Daniel Dupret, Jean-Michel Masson, Fabrice Lefevre
-
Patent number: 7897336Abstract: The present invention describes a method for identification and labeling of sentinel lymph nodes (SLNs) and the presence or absence of lymph node metastases as an important diagnostic and prognostic factor in early stage cancers of all types. The method, know as Molecular Lymphatic Mapping, uses traditional dye/radioactive tracer based techniques in conjunction with a nucleic acid marker to identify and label the SLN, not only for current diagnostic methods, but for archival purposes. In addition, MLM can be used to deliver a therapeutic gene or genes to the SLN to activate tumor immunity to tumor cells, and/or to inhibit tumor metastases. The methods may be combined with therapeutic intervention including chemotherapy and radiotherapy.Type: GrantFiled: August 15, 2003Date of Patent: March 1, 2011Assignee: John Wayne Cancer InstituteInventors: Dave S. B. Hoon, Bret Taback
-
Patent number: 7803528Abstract: A method is provided for detecting the presence of nucleotides or monitoring nucleotide amplification. It utilizes fluorescence energy transfer by competitive hybridization. Competitive hybridization is achieved by using unequal length complementary probes which have a fluorophore on one probe and a quencher on the other. The fluorophore and quencher are juxtaposed in a manner wherein the proximity of the quencher to the fluorophore produces quenching of the fluorescence of the fluorphore.Type: GrantFiled: February 26, 1998Date of Patent: September 28, 2010Assignee: Quest Diagnostics IncorporatedInventors: Chih-Sheng Chiang, Jose F Cuan
-
Patent number: 7709192Abstract: A nucleic acid ligand “biochip” is disclosed, consisting of a solid support to which one or more specific nucleic acid ligands is attached in a spatially defined manner. Each nucleic acid ligand binds specifically and avidly to a particular target molecule contained within a test mixture, such as a bodily fluid. The target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the test mixture with the biochip leads to the binding of a target molecule to its cognate nucleic acid ligand. The biochip may then be contacted with a reagent(s) that reacts covalently with proteins and not with nucleic acids. Each protein target in the test mixture may then detected by detecting the presence of the reagent at the appropriate address on the biochip.Type: GrantFiled: February 27, 2003Date of Patent: May 4, 2010Assignee: SomaLogic, Inc.Inventors: Larry Gold, Dominic Zichi
-
Patent number: 7429376Abstract: The invention provides for methods of screening for compounds that increase the expression of P-selectin, SDF-1, and/or CXCR4 on facilitatory cells (FCs). The invention also provides for methods of screening for compounds that increase the level of p-predendritic cells (p-pre DC) without substantially decreasing the level of natural killer (NK) cells in a population of FCs. The invention further provides for methods of characterizing the facilitating capability of FCs by evaluating such cells for the amount of P-selectin, SDF-1, and/or CXCR4.Type: GrantFiled: October 17, 2005Date of Patent: September 30, 2008Assignee: University of Louisville Research Foundation, Inc.Inventor: Suzanne T. Ildstad
-
Patent number: 7368243Abstract: The present invention provides a method of detecting abnormal serum nucleic acid profiles to assess the risk of a transmissible spongiform encephalopathy, e.g., BSE.Type: GrantFiled: July 8, 2005Date of Patent: May 6, 2008Assignee: Chronix BiomedicalInventors: Ekkehard Schuetz, Leonid Iakoubov, Howard Urnovitz
-
Patent number: 7282328Abstract: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.Type: GrantFiled: September 19, 2003Date of Patent: October 16, 2007Assignee: New England Biolabs, Inc.Inventors: Huimin Kong, Myriam Vincent, Yan Xu
-
Patent number: 7183395Abstract: Provided are methods of identifying oligonucleotides having transcriptional or translational activity by integrating the oligonucleotide into a eukaryotic cell genome such that the oligonucleotide is operatively linked to an expressible polynucleotide, and detecting a change in expression of the expressible polynucleotide due to the operatively linked oligonucleotide. Also provided are vectors useful for identifying an oligonucleotide having transcriptional or translational regulatory activity according to a method of the invention. In addition, isolated synthetic transcriptional or translational regulatory elements identified according to a method of the invention are provided, as are kits, which contain a vector useful for identifying a transcriptional or translational regulatory element, or an isolated synthetic transcriptional or translational regulatory element or plurality of such elements. Also provided are isolated transcriptional regulatory elements.Type: GrantFiled: January 26, 2001Date of Patent: February 27, 2007Assignee: The Scripps Research InstituteInventors: Vincent P. Mauro, Gerald M. Edelman, Stephen A. Chappell, Frederick S. Jones, Geoffrey Owens, Robyn Meech
-
Patent number: 7135055Abstract: The present invention relates to composite core/shell nanoparticles and a two-step method for their preparation. The present invention further relates to biomolecule-core/shell nanoparticle conjugates and methods for their preparation. The invention also relates to methods of detection of biomolecules comprising the biomolecule or specific binding substance-core/shell nanoparticle conjugates.Type: GrantFiled: March 26, 2003Date of Patent: November 14, 2006Assignee: Nanosphere, Inc.Inventors: Chad A. Mirkin, Yun-Wei Cao, Rongchao Jin
-
Patent number: 7118907Abstract: A microfluidic system is provided that includes a substrate, a first microchannel disposed in the substrate for providing a reactant to a reaction zone, a second microchannel disposed in the substrate, and a third microchannel disposed in the substrate, the third microchannel providing fluid communication between the first and second microchannels. The system also typically includes first and second electrodes, positioned at opposite ends of the second microchannel, for providing an electric field within the second microchannel. In operation, when the reactant is in the reaction zone, a reaction product is produced having a net electric charge different from the electric charge of the reactant.Type: GrantFiled: June 5, 2002Date of Patent: October 10, 2006Assignee: Li-Cor, Inc.Inventors: John G. K. Williams, Gregory R. Bashford
-
Patent number: 7112423Abstract: The present invention provides methods and kits for amplifying target nucleic acids (including whole genomes) using nicking agents. In certain aspects, the amplification does not require the use of any external oligonucleotide primers that are capable of annealing to a portion of the target nucleic acid. This invention is useful in many areas such as genetic disease diagnoses, forensic analyses and palcoarcheological studies.Type: GrantFiled: July 15, 2002Date of Patent: September 26, 2006Assignee: Keck Graduate InstituteInventors: Jeffrey Van Ness, David J Galas, Lori K Van Ness
-
Patent number: 7105318Abstract: Method for the detection of a nucleic acid comprising the production of a plurality of amplificates of a section of this nucleic acid with the aid of two primers, one of which can bind to a binding sequence A of the nucleic acid and the other can bind to a binding sequence C? which is complementary to a sequence C which is located in the 3? direction from A and does not overlap with A, contacting the amplificates with a probe having a binding sequence D which can bind to a sequence B which is located between the sequences A and C or to the complement thereof, and detecting the formation of a hybrid of the amplificate and probe where the sequence located between the binding sequences A and C contains no nucleotides that do not belong to the binding sequence D of the probe or its complement D?.Type: GrantFiled: December 17, 2002Date of Patent: September 12, 2006Assignee: Roche Diagnostics GmbHInventors: Christoph Kessler, Gerd Haberhausen, Knut Bartl, Henrik Orum
-
Patent number: 7056716Abstract: The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.Type: GrantFiled: March 15, 2001Date of Patent: June 6, 2006Assignee: Invitrogen CorporationInventors: Robert Jason Potter, Kim Rosenthal
-
Patent number: 7037653Abstract: The invention concerns methods for detecting guar gum alone or in mixtures of guar gum and locust bean gum. The invention also concerns methods for extracting, amplifying, and detecting DNA of guar and locust bean gums and the mixtures thereof. DNAs of plants from which guar and locust bean gums are extracted are amplified by means of polymerase chain reaction (PCR) using conserved initiators. Differences in the sequences of the amplification products obtained from these two plants enable their differentiation, the identification of guar DNA in mixtures of guar and locust bean gum mixtures and the design of guar-specific PCR initiators which detect guar only in mixtures of guar and locust bean gum.Type: GrantFiled: March 2, 2001Date of Patent: May 2, 2006Assignee: Consejo Superior de Investigaciones CientificasInventors: Vicente Javier Benedí Benito, Antonio Doménech Sánchez, María Luz Hernández Viadel, Sebastián Albertí Serrano, Josep Antoni Rosselló Picornell
-
Patent number: 7033760Abstract: The present invention is directed to solid supports having metallic surfaces comprising blocking moieties and modified nucleic acids, which exhibit excellent characteristics in hybridization assays, in a stable, reproducible, rapid manner. In an additional aspect, the invention provides methods utilizing the solid supports to hybridize probe nucleic acid to target nucleic acid and methods for detecting the hybridization complex.Type: GrantFiled: August 3, 2001Date of Patent: April 25, 2006Assignee: California Institute of TechnologyInventors: Thomas J. Meade, Jon F. Kayyem
-
Patent number: 7022478Abstract: This invention relates to the use of a blood sample from a patient for evaluating stroke or cardiac ischemia in the patient.Type: GrantFiled: July 11, 2002Date of Patent: April 4, 2006Assignee: The Chinese University of Hong KongInventors: Timothy Hudson Rainer, Yuk Ming Dennis Lo, Yuk Lan Lam, Lawrence Ka Sing Wong
-
Patent number: 7015372Abstract: The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited plant artificial chromosomes (PLACs) which can serve as vectors for the construction of transgenic plant and animal cells. In addition, information on the structure and function of these regions will prove valuable in isolating additional centromeric and centromere related genetic elements and polypeptides from other species.Type: GrantFiled: August 6, 2002Date of Patent: March 21, 2006Assignee: University of ChicagoInventors: Daphne Preuss, Gregory Copenhaver
-
Patent number: 6994962Abstract: The invention relates to a method for identifying inherited point mutations in a targeted region of the genome in a large population of individuals and determining which inherited point mutations are deleterious, harmful or beneficial. Deleterious mutation are identified directly by a method of recognition using the set of point mutations observed in a large population of juveniles. Harmful mutations are identified by comparison of the set of point mutation observed in a large set of juveniles and a large set of aged individuals of the same population. Beneficial mutations are similarly identified.Type: GrantFiled: February 14, 2000Date of Patent: February 7, 2006Assignee: Massachusetts Institute of TechnologyInventor: William G. Thilly
-
Patent number: 6982146Abstract: A method and device is disclosed for high speed, automated sequencing of nucleic acid molecules. A nucleic acid molecule to be sequenced is exposed to a polymerase in the presence of nucleotides which are to be incorporated into a complementary nucleic acid strand. The polymerase carries a donor fluorophore, and each type of nucleotide (e.g. A, T/U, C and G) carries a distinguishable acceptor fluorophore characteristic of the particular type of nucleotide. As the polymerase incorporates individual nucleic acid molecules into a complementary strand, a laser continuously irradiates the donor fluorophore, at a wavelength that causes it to emit an emission signal (but the laser wavelength does not stimulate the acceptor fluorophore). In particular embodiments, no laser is needed if the donor fluorophore is a luminescent molecule or is stimulated by one.Type: GrantFiled: August 29, 2000Date of Patent: January 3, 2006Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Thomas D. Schneider, Denise Rubens