Patents Examined by Teresa Wessendorf
-
Patent number: 8916504Abstract: The present invention features improved methods of in vitro RNA display to allow reliable expression and selection of scFv antibody molecules from expression libraries. The improved methods, in part, involve the use of mildly reducing conditions, which favor of scFv intra-chain disulphide bond and thus correct folding of the scFv antibody molecules. Although particularly suited to expression and selection of scFv antibody molecules, the methods of the invention are also expedient for in vitro RNA display of all classes of protein.Type: GrantFiled: September 30, 2009Date of Patent: December 23, 2014Assignee: Abbvie Inc.Inventors: Chung-Ming Hsieh, Yuliya A. Kutskova, John E. Memmott
-
Patent number: 8916357Abstract: Methods for identifying a compound capable of interacting with a Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance, adding test cells expressing a RTK to the device, measuring first impedances, adding a compound to at least one compound well and adding a vehicle control to at least one control well, measuring second impedances of the compound well and the control well, determining the change in the impedance for the compound well and the one control well, comparing the change in impedance between the compound well and the control well, and identifying the compound interacts with the RTK if the comparison demonstrates a significant difference between the change in impedance.Type: GrantFiled: August 7, 2012Date of Patent: December 23, 2014Assignee: ACEA Biosciences, Inc.Inventors: Yama A. Abassi, Naichen Yu, Josephine Atienza, Xiao Xu, Xiaobo Wang
-
Patent number: 8871688Abstract: The present invention relates to a method for absolute quantification of polypeptides.Type: GrantFiled: June 2, 2008Date of Patent: October 28, 2014Assignees: INSERM (Institut National de la Sante et de la Recherche Medicale), Commissariat a l'Energie AtomiqueInventors: Virginie Brun, Alain Dupuis, Jerome Garin
-
Patent number: 8796183Abstract: A method of screening for template-fixed ?-hairpin mimetics and libraries including a plurality of these mimetics is provided. The template-fixed ?-hairpin mimetics are of the following general formula: R1-Cys-Z-Cys-R2??I wherein the two cysteine residues are bridged by a disulfide bond, thereby forming a cyclic peptide; R1 and R2 are di- or tripeptide moieties of certain types, as defined herein; and Z is a chain of n amino acid residues with n being an integer from 4 to 20 and with each of these n amino acid residues being, independently, derived from any naturally occurring L-?-amino acid.Type: GrantFiled: August 2, 2011Date of Patent: August 5, 2014Assignees: Polyphor Ltd., Universität ZürichInventors: Jan Wim Vrijbloed, Daniel Obrecht, Sergio Locurio, Frank Otto Gombert, Christian Ludin, Françoise Jung
-
Patent number: 8759259Abstract: Embodiments of the invention are directed to a one-bead-two-compound method for the creation of encoded cyclic peptoid libraries. This scheme is useful for the creation of cyclic peptoid microarrays since only the cyclic peptoid, not the linear encoding molecule, contains an attachment residue and thus can be spotted onto an activated substrate.Type: GrantFiled: October 15, 2010Date of Patent: June 24, 2014Assignee: The Board of Regents of the University of Texas SystemInventors: Yong-Uk Kwon, Thomas Kodadek
-
Patent number: 8754193Abstract: A family of GFP scaffolds capable of accommodating two proximal, randomized binding loops is disclosed. GFP-based binders binding with nanomolar affinity are developed from a library of these GFP scaffolds.Type: GrantFiled: May 2, 2012Date of Patent: June 17, 2014Assignee: Wisconsin Alumni Research FoundationInventors: Eric V. Shusta, Tej Pavoor
-
Patent number: 8741814Abstract: A proteinaceous particle, for example a bacteriophage, ribosome or cell, displaying on its surface a T-cell receptor (TCR). The displayed TCR is preferably a heterodimer having a non-native disulfide bond between constant domain residues. Such display particles may be used for the creation of diverse TCR libraries for the identification of high affinity TCRs. Several high affinities are disclosed.Type: GrantFiled: October 21, 2009Date of Patent: June 3, 2014Assignees: Immunocore Limited, Adaptimmune LimitedInventors: Bent Karsten Jakobsen, Torben Bent Andersen, Peter Eamon Molloy, Yi Li, Jonathan Michael Boulter
-
Patent number: 8680020Abstract: Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis.Type: GrantFiled: July 15, 2009Date of Patent: March 25, 2014Assignee: Academia SinicaInventors: Chi-Huey Wong, Chung-Yi Wu, Susan Y. Tseng
-
Patent number: 8658388Abstract: The present invention provides antibody-based arrays for detecting the activation state and/or total amount of a plurality of signal transduction molecules in rare circulating cells and methods of use thereof for facilitating cancer prognosis and diagnosis and the design of personalized, targeted therapies.Type: GrantFiled: March 11, 2008Date of Patent: February 25, 2014Assignee: Nestec S.A.Inventors: Jeanne Harvey, Sharat Singh, Phillip Kim, Xinjun Liu, Robert Barham, Limin Liu
-
Patent number: 8648015Abstract: The present invention provides methods for protein engineering. Specifically, the invention provides methods utilizing site evaluation libraries to design libraries that optimize two or more properties of a protein.Type: GrantFiled: May 12, 2010Date of Patent: February 11, 2014Assignee: Danisco US Inc.Inventors: David A. Estell, Wolfgang Aehle
-
Patent number: 8637436Abstract: A biosensor array, system and method for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.Type: GrantFiled: August 24, 2007Date of Patent: January 28, 2014Assignee: California Institute of TechnologyInventor: Arjang Hassibi
-
Patent number: 8618024Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.Type: GrantFiled: August 31, 2006Date of Patent: December 31, 2013Assignee: Gendaq LimitedInventors: Yen Choo, Aaron Klug, Mark Isalan
-
Patent number: 8597938Abstract: Provided are methods and oligonucleotides useful as primers and templates for internal controls designed for use in Real Time Reverse Transcriptase Polymerase Chain Reactions. Use of the present methods and oligonucleotides allows validation of assay parameters and of the results that an assay set.Type: GrantFiled: October 10, 2008Date of Patent: December 3, 2013Assignee: QIAGEN Sciences LLCInventors: Jingping Yang, Li Shen
-
Patent number: 8586340Abstract: The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2] cycloaddition reactions and Staudinger modifications.Type: GrantFiled: October 11, 2006Date of Patent: November 19, 2013Assignee: The Scripps Research InstituteInventors: Meng-Lin Tsao, Feng Tian, Peter Schultz
-
Patent number: 8399383Abstract: The present invention relates to protein chips useful for the large-scale study of protein function where the chip contains densely packed reaction wells. The invention also relates to methods of using protein chips to assay simultaneously the presence, amount, and/or function of proteins present in a protein sample or on one protein chip, or to assay the presence, relative specificity, and binding affinity of each probe in a mixture of probes for each of the proteins on the chip. The invention also relates to methods of using the protein chips for high density and small volume chemical reactions. Also, the invention relates to polymers useful as protein chip substrates and methods of making protein chips. The invention further relates to compounds useful for the derivatization of protein chip substrates.Type: GrantFiled: May 4, 2001Date of Patent: March 19, 2013Assignee: Yale UniversityInventors: Michael Snyder, Mark Reed, Heng Zhu, James Frank Klemic
-
Patent number: 8372949Abstract: The present invention includes methods for selective binding of inorganic materials and the compositions that made up of the selecting agent and the target materials. One form of the present invention is a method for selecting crystal-binding peptides with binding specificity including the steps of contacting one or more amino acid oligomers with one or more single-crystals of a semiconductor material so that the oligomers may bind to the crystal and eluting the bound amino acid oligomers from the single-crystals.Type: GrantFiled: July 24, 2007Date of Patent: February 12, 2013Assignee: The Board of Regents, The University of Texas SystemInventor: Angela M. Belcher
-
Patent number: 8242058Abstract: Methods and reagents for site-selective functionalization of peptides and proteins. The methods most generally involve the reaction of a thioester with hydrazine. Reagents include bifunctional reagents of formula: H2N—NH—CH2-M-L-FG and salts thereof where M is a single bond or a chemical group carrying a non-bonding electron pair, such as —C(O)NR?—, where R? is H, or an alkyl or aryl group; L is an optional linker group as described above; and FG is a functional group having reactivity that is orthongonal to that of the hydrazine group. FG can, among others, be an azide, alkenyl, alkynyl, nitrile (—CN) or triazole group and is preferably an azide group (—N3). Methods and reagents can, for example, be combined with intein-mediated protein splicing to link proteins or fragments thereof to various chemical species or to a surface.Type: GrantFiled: July 23, 2007Date of Patent: August 14, 2012Assignee: Wisconsin Alumni Research FoundationInventors: Ronald T. Raines, Jeet Kalia
-
Patent number: 7855279Abstract: The present invention provides unstructured recombinant polymers (URPs) and proteins containing one or more of the URPs. The present invention also provides microproteins, toxins and other related proteinaceous entities, as well as genetic packages displaying these entities. The present invention also provides recombinant polypeptides including vectors encoding the subject proteinaceous entities, as well as host cells comprising the vectors. The subject compositions have a variety of utilities including a range of pharmaceutical applications.Type: GrantFiled: March 6, 2007Date of Patent: December 21, 2010Assignee: Amunix Operating, Inc.Inventors: Volker Schellenberger, Willem P. Stemmer, Chia-wei Wang, Michael D. Scholle, Mikhail Popkov, Nathaniel C. Gordon, Andreas Crameri