Patents Examined by Thomas G. Wiseman
  • Patent number: 4755464
    Abstract: A method for the preparation of plasmid DNA and the expression products thereof. The method provides cells, enriched in plasmid DNA and expression products thereof, which comprise chromosomal DNA which has been substantially degraded by exposing a plasmid DNA-containing organism to an effective dose of ionizing radiation.
    Type: Grant
    Filed: April 23, 1984
    Date of Patent: July 5, 1988
    Assignee: Gentech Australia Limited
    Inventors: Donald G. MacPhee, Anthony J. Radford, Darryl C. Reanney
  • Patent number: 4753884
    Abstract: The present invention relates to pseudorabies virus mutants containing deletion and/or insertion mutations in a major viral glycoprotein gene, such that no antigenic polypeptides encoded by the viral gene are produced. As a result, animals vaccinated with such do not develop antibodies to the viral glycoprotein and can be distinguished from animals infected with pseudorabies virus field strains and known pseudorabies virus vaccine strains. The present invention also relates to vaccines for pseudorabies disease containing the same, methods for production of the same and methods for use of the same.
    Type: Grant
    Filed: September 10, 1987
    Date of Patent: June 28, 1988
    Assignees: NovaGene, Inc., Baylor College of Medicine
    Inventors: Malon Kit, Saul Kit
  • Patent number: 4753879
    Abstract: Modified human tissue plasminogen activators, the DNA sequences coding on expression for them and methods of making them in hosts transformed with those DNA sequences. The single chain form of these modified human t-PAs, t-PA (Lys 277.fwdarw.X), has a longer half life than the single chain form of native, or recombinant, t-PA, yet, in the two chain form these modified t-PAs have substantially the same affinity for fibrin and substantially the same activity as the two chain form of native, or recombinant, t-PA.
    Type: Grant
    Filed: August 27, 1984
    Date of Patent: June 28, 1988
    Assignee: Biogen N.V.
    Inventors: Joseph J. Rosa, Margaret D. Rosa
  • Patent number: 4753880
    Abstract: A novel method of selecting Streptomyces recombinant DNA-containing host cells and vectors useful in the method are described. The vectors confer spiramycin resistance to sensitive Streptomyces host cells and thus provide a convenient method of selecting Streptomyces transformants. The novel spiramycin resistance-conferring gene described can be isolated on an .about.3.8 kb Bc1I restriction fragment from plasmid pNAS105. Plasmid pNAS105 can be isolated from Streptomyces griseofuscus C581/pNAS105 (NRRL 15919).
    Type: Grant
    Filed: December 24, 1984
    Date of Patent: June 28, 1988
    Assignee: Eli Lilly and Company
    Inventors: Nancy A. Schaus, Patricia J. Solenberg
  • Patent number: 4752582
    Abstract: Cloned mouse hybridoma cell lines have been established which continuously produce antibodies that are highly specific to and exhibit high affinity for glycophorin A.sup.N and differentiate between the M and N forms of human glycophorin A.
    Type: Grant
    Filed: November 29, 1984
    Date of Patent: June 21, 1988
    Assignee: The United States of America as represented by the United States Department of Energy
    Inventors: Martin Vanderlaan, William L. Bigbee, Ronald H. Jensen, Stella S. N. Fong, Richard G. Langlois
  • Patent number: 4752576
    Abstract: Methods and compositions are provided for high level production of foreign proteins in yeast. The system is exemplified by production of .alpha..sub.1 -antitrypsin in yeast strain AB110, derived from yeast strains 2150-2-3 and AB103.1.S. carlsbergensis/S. cerevisiae hybrid strain AB110 containing plasmid pCl/1GAPATi9 was deposited at the A.T.C.C. on May 9, 1984, and given Accession No. 20709.
    Type: Grant
    Filed: June 14, 1984
    Date of Patent: June 21, 1988
    Assignee: Chiron Corporation
    Inventors: Anthony J. Brake, Robert A. Hallewell, Steven Rosenberg
  • Patent number: 4752581
    Abstract: A fibrinolytically active hybrid protein which comprises one chain of a 2-chain protease linked to a chain of a different 2-chain protease, or to the same chain of the same protease, at least one of the chains in the hybrid protein being derived from a fibrinolytically active protease, such that the hybrid protein has a catalytic site essential for fibrinolytic activity which is optionally blocked by a removable blocking group.
    Type: Grant
    Filed: December 21, 1984
    Date of Patent: June 21, 1988
    Assignee: Beecham Group p.l.c.
    Inventors: Jeffery H. Robinson, Ian Dodd
  • Patent number: 4751348
    Abstract: Plant cell lines with altered flower structures, and altered plant male and/or female sterility/fertility, and altered polyamine synthesis have been isolated using a UV mutagenizing light, polyamine synthesis inhibitors, and growth in the dark. Some of these cell lines are resistant to MGBG and exhibit elevated polyamine synthesis. Regenerated plants with unusual flowers are thus possible as are crop and flower plants with unusual flowers, and/or altered fertility capability by the method of the invention. These plant lines are also useful as research tools.
    Type: Grant
    Filed: July 16, 1983
    Date of Patent: June 14, 1988
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Russell L. Malmberg, Jean McIndoo
  • Patent number: 4751180
    Abstract: Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S.
    Type: Grant
    Filed: March 28, 1986
    Date of Patent: June 14, 1988
    Assignee: Chiron Corporation
    Inventors: Lawrence S. Cousens, Patricia A. Tekamp-Olson, Jeffrey R. Shuster, James P. Merryweather
  • Patent number: 4748118
    Abstract: A process for constructing the plasmids pDR416 and pDR420 which contain arsenic resistance genes and which are able to replicate in both T. ferroxidans and E. coli comprises preparing a recombinant of pBR325 or the like and a T. ferrooxidans plasmid, forming a deletion plasmid and inserting arsenic resistance genes e.g. from R46 by EcoR1 excision and ligation.
    Type: Grant
    Filed: November 1, 1984
    Date of Patent: May 31, 1988
    Assignee: General Mining Union Corporation Limited
    Inventors: Douglas E. Rawlings, David R. Woods
  • Patent number: 4748021
    Abstract: A biological control agent comprising the antifungal agent Trichoderma harzianum T-35 (ATCC No. 20691), which is characterized by antifungal activity against fungi of the genus Fusarium. This strain is useful for protecting most crops affected by the fungus Fusarium spp. and is more active than other previously disclosed strains. Biocontrol compositions containing T. harzianum T-35 (ATCC No. 20691) provide antifungal protection to a broad spectrum of plants, including wheat, cotton, melons and tomatoes.
    Type: Grant
    Filed: July 27, 1984
    Date of Patent: May 31, 1988
    Assignee: Yissum Research and Development Company of the Hebrew University of Jerusalem
    Inventors: Ilan Chet, Alex Sivan
  • Patent number: 4748120
    Abstract: Biological compositions are freed of functional polynucleotides by treatment of the biological composition with psoralen derivatives under irradiation conditions in which the proteins retain their original physiological activities and any polynucleotide present is rendered inactive.
    Type: Grant
    Filed: October 20, 1986
    Date of Patent: May 31, 1988
    Assignee: Diamond Scientific Co.
    Inventor: Gary P. Wiesehahn
  • Patent number: 4746511
    Abstract: A lipopolysaccharide characterized by a polysaccharide portion composed of D-arabinose and D-mannose in a 1:3/4 ration and 37 to 47% of a fatty acid portion having 14-19 carbon atoms bonded to the polysaccharide through an ester linkage. This lipopolysaccharide has physiological activities such as antitumor activity, immunizing activity, cell juvenescent activity, phagocyte activating activity, and infection preventing activity.This lipopolysaccharide is prepared by culturing a Mycobacterium or a Propionibacterium and extracting the lipopolysaccharide from the culture with a non-ionic surface active agent and purifying the extract with a molecular sieve.
    Type: Grant
    Filed: July 28, 1986
    Date of Patent: May 24, 1988
    Assignees: Chisato Maruyama, Zeria Shinyaku Kogyo Kabushiki Kaisha
    Inventors: Hiroshi Kobatake, Takahiro Suekane, Kazuhiro Kumagai, Osamu Ohya
  • Patent number: 4746539
    Abstract: It has previously been shown that the serum from patients suffering from a wide range of cancers contains a cancer marker protein having the ability to release RNA from cell nuclei. This cancer marker protein is purified by taking the protein fraction precipitating between 30% and 50% saturated aqueous ammonium sulfate solution, dialyzing a solution of the protein fraction against TMK buffer, chromatographing the dialyzed protein on a molecular sieve and isolating the fraction having a molecular weight of about 60,000; and removing albumin. Injection of the purified protein into rabbits, preparation of serum from blood of the rabbits and absorption of the sera with normal plasma fraction produces at antibody specific to the cancer marker protein and therefore useful in a radioimmunoassay or ELISA test for a wide variety of cancers.
    Type: Grant
    Filed: November 23, 1983
    Date of Patent: May 24, 1988
    Assignee: The Ohio State University Research Foundation
    Inventors: Thomas E. Webb, Dorothy E. Schumm, Margaret Hanausek-Walaszek
  • Patent number: 4746609
    Abstract: The present invention teaches restriction endonuclease Asp 718 which cleaves DNA in the palindromic recognition sequence at the sites indicated by the arrow: ##STR1## The endonuclease, obtained from Achromobacter species 718, DSM 2969, is useful in obtaining DNA fragments, e.g., for determining nucleotide sequences and other types of analysis of DNA.
    Type: Grant
    Filed: June 10, 1985
    Date of Patent: May 24, 1988
    Assignee: Boehringer Mannheim GmbH
    Inventors: Bryan Bolton, Michael J. Comer, Christoph Kessler, Georg Nesch
  • Patent number: 4745057
    Abstract: Disclosed are a novel method for inducing the high expression of a nucleotide sequence which is under the transcriptional and translational control of the yeast YG100 gene and the novel vectors, transformants and selectable DNA for the practice thereof.
    Type: Grant
    Filed: August 13, 1984
    Date of Patent: May 17, 1988
    Assignee: Eli Lilly and Company
    Inventors: Cheryl A. Beckage, Thomas D. Ingolia
  • Patent number: 4745062
    Abstract: The present invention relates to a plasmid vector for cloning and expression of a protein in a microorganism, which comprises at least one structural gene which codes the synthesis of the said protein and elements which ensure the expression of the said structural gene in a microorganism, and wherein the promotion of the structural gene is ensured by the expression promotor of the .beta.-glucosidase gene in yeasts.The present invention also relates to microorganisms transformed by the said vectors, in particular a transformed strain of S. cerevisiae, a fermentation process using the said vectors, and the enzymes prepared by the said process, in particular .beta.-glucosidase.
    Type: Grant
    Filed: December 5, 1984
    Date of Patent: May 17, 1988
    Assignee: Centre National de la Recherche Scientifique
    Inventors: Michel M. Guerineau, Alain Raynal
  • Patent number: 4745056
    Abstract: Cloning vectors are disclosed to obtain secretion of a desired protein from a host Streptomyces when a structural gene coding for the protein is inserted into the vector. The vector has: (1) regulatory DNA that includes a promoter sequence effective to start transcription in the host Streptomyces and DNA that encodes a ribosome-binding site; (2) a DNA sequence that codes for a signal sequence that occurs naturally in a Streptomyces strain or that derives from such a DNA sequence; and (3) at least one engineered restriction endonuclease recognition site positioned for the insertion of a structural gene, the DNA that encodes a signal sequence and attached structural gene being transcribed and translated together under the control of the regulatory DNA. Expression vectors are disclosed which include a structural gene coding for a desired protein so positioned. Streptomyces cells containing the vector and methods of using them to produce the desired protein are disclosed.
    Type: Grant
    Filed: October 23, 1984
    Date of Patent: May 17, 1988
    Assignee: Biotechnica International, Inc.
    Inventors: Sonia Guterman, Janice Pero, Phillips Robbins
  • Patent number: 4745064
    Abstract: The invention relates to a novel microorganism of the genus Rhodococcus, to the use thereof in a process for the microbiological degradation of amino-s-triazines and cyanuric acid in wastewaters, as well as to a process for the microbiological degradation of s-triazine derivatives to biomass or to degradation products such as NH.sup.30.sub.4 and/or chloride, which comprises the combined use of said microorganism with Pseudomonas spp.
    Type: Grant
    Filed: October 26, 1984
    Date of Patent: May 17, 1988
    Assignee: Ciba-Geigy Corporation
    Inventors: Alasdair M. Cook, Ralf Hutter
  • Patent number: 4745065
    Abstract: Disclosed is an autoreplicative plasmid containing DNA which encodes herbicide resistance, such that the plasmid is capable of transforming a yeast cell to become resistant to one or more organic herbicide growth inhibitors. Also disclosed is a process for the selective culture of yeasts transformed by the plasmid.
    Type: Grant
    Filed: May 8, 1984
    Date of Patent: May 17, 1988
    Assignee: Labofina, S.A.
    Inventors: Jean M. A. G. Delcour, Anne-Marie J. C. G. Colson-Corbisier, Annie F. J. De Baetselier-van Broekhoven, Charles Colson