Abstract: A method for obtaining E. coli cell lines which carry the deoR mutation is described, as well as the cell lines themselves. These cell lines are useful in cell transfection and transformation, as they transfect transform at much higher frequencies than the previously available cell lines.
Abstract: The present invention presents a DNA containing a synthetic gene for expression of human epidermal cell growth factor of the DNA sequence; ##STR1## The adoption of the DNA of the present invention improves the stability of mRNA, the translation efficiency and the influence of the high-dimensional structure which is determined by the mRNA sequence, and made it possible to produce hEGF efficiently.
Abstract: A method is provided for transferring genes into cells which comprises the step of subjecting the mixture of genes to be transferred and the target cells to an electric treatment.
Abstract: The cDNA of a new proteinase inhibitor was isolated from a cDNA library of human liver biopsy material using a synthetic Oligonucleotide probe. The cDNA can be expressed by introduction of the cDNA to a microbial host on a DNA vector. Analogs of known proteinase inhibitors can be producing by alteration of the cDNA by substituting codons in the active center of the new proteinase inhibitor.
Abstract: Method of treating a tumor in a mammal comprises administering to said mammal an effective anti-tumor amount of proteases originating from microorganisms.Method of treating a tumor in a mammal comprises administering to said mammal an effective anti-tumor amount of a protease originating from a microorganism which protease is chemically modified by one of the following procedures:(a) coupling with a saccharide,(b) introduction of a hydrophobic polymeric group,(c) alteration of electric charge of the protein surface,(d) conjugation with a low molecular weight anti-tumor agent of molecular weight less than 2,000,(e) formation of dimer or oligomer by cross-linking of protease molecules,(f) conjugation with a synthetic polycation,(g) conjugation with a synthetic polyanion, and(h) combination of the above-mentioned procedures.
Abstract: The invention relates to a process for the isolation and purification of .alpha.-interferons by (a) dissolution of the interferon-containing crude product in guandine. HCl solution and precipitation by dilution with water and/or (b) by chromatography on an HIC column under renaturing conditions.
Abstract: A process is provided for producing an ordered series of cloned, circular, DNA molecules containing shortened target DNA segments derived from a long target DNA segment, which are suitable for use in determining the nucleotide sequence of the long target DNA segment, or for targeting specific regions within the target DNA segment. The process includes producing, by molecular cloning, a plurality of double-stranded recombinant DNA molecules each containing: (i) vector DNA; (ii) a sequencing primer binding site; and, (iii) a DNA region having unique endonuclease sites and a long target DNA segment. The sequencing primer binding site is spaced from the long target DNA segment by at least a portion of said DNA region having the unique endonuclease sites. The plurality of double-stranded circular recombinant DNA molecules are cleared using two restriction endonucleases.
Abstract: The present invention teaches a restriction endonuclease which recognizes and cleaves palindromic sequence5'Pu G G N C C Py 3'3'Py C C N G G Pu 5'as well as a process for obtaining this endonuclease. One source of the endonuclease is Deinococcus radiophilus DSM 20551 (ATCC 27063). The endonuclease is useful in analysis of DNA molecules.
Abstract: Disclosed are compounds of the formula ##STR1## wherein R represents hydrogen or C.sub.1 -C.sub.4 -alkyl. These compounds are obtained by fermentation of the known saframycin A producing strain Streptomyces lavendulae No. 314 and by reaction with the amino acids L-tyrosine, L-methionine, glycine and HOOC-(CH)NH.sub.2 -CH.sub.2 -R. When R is hydrogen, saframycin Y.sub.3 and when R is methyl, saframycin Y.sub.d-1 are produced.
Abstract: Regulation of eucaryotic gene expression is controlled by procaryotic peptides. The peptides recognize specific DNA sequences present in the gene, which may be derived from procaryotic genes, and either activate or repress gene transcription. Hybrid procaryotic peptides may be used containing both repressor and activator peptides.
Type:
Grant
Filed:
December 12, 1985
Date of Patent:
May 23, 1989
Assignee:
President and Fellows of Harvard College
Abstract: The subject invention concerns a novel plasmid and its use in a microbial host to degrade a variety of organic compounds. Some of these compounds, such as ethylene dichloride, are undesirable waste products found in various dump sites. The invention also concerns a novel microbe hosting the novel plasmid. The novel plasmid has been shown to encode the gene(s) responsible for the degradation of the organic compounds. Thus, microbes hosting this plasmid, denoted pEDC, can be used to degrade ethylene dichloride, and other compounds.
Abstract: A restriction enzyme, ApaLI, which recognizes the base sequence in a double-stranded deoxyribonucleic acid molecule as shown below, and cleaves it at the arrow-marked sites, ##STR1## wherein A, G, T and C represent adenosine, guanosine, thymidine and cytidine, respectively. Also provided is a process for producing this enzyme, by growing a microorganism belonging to the genus Acetobacter.
Abstract: Bacillus strains having reduced levels of extracellular protease are produced by replacing the native chromosomal DNA sequence comprising the gene for an extracellular protease, such as subtilisin, with a partially homologous DNA sequence having an inactivating DNA segment inserted therein. The strains are useful as hosts for the expression and secretion of heterologous polypeptides or proteins.
Type:
Grant
Filed:
May 13, 1985
Date of Patent:
May 9, 1989
Assignee:
Genex Corporation
Inventors:
Stephen R. Fahnestock, Kathryn E. Fisher
Abstract: The strain Streptomyces coelicolor DSM 3030 excretes into the fermentation medium high yields of a bacteriolytic enzyme product which is very active against Gram-positive and Gram-negative bacteria. Preferred fermentation media contain sugar beet molasses and/or calcium ions.
Type:
Grant
Filed:
November 6, 1985
Date of Patent:
May 9, 1989
Assignee:
Hoechst Aktiengesellschaft
Inventors:
Gerhard Wohner, Hartmut Voelskow, Paul Prave, Erich Luck, Gert-Wolfhard von Rymon Lipinski
Abstract: The present invention discloses a biologically active basement membrane composition. When polymerized under physiological conditions, the composition forms gel-like structures whose ultrastructure resembles interconnected thin sheets of the lamina densa zone of basement membrane. The major components of the composition include laminin, type IV collagen, heparin sulfate proteoglycan, entactin and nidogen. These components polymerize in constant proportions when redissolved and allowed to reconstitute. Molecular sieve studies on the soluble extract demonstrate that laminin, entactin and nidogen are associated in a large but dissociable complex. The reconstituted matrix is biologically active and stimulates the growth and differentiation of a variety of cells, including epithelial cells, nerve cells, hair follicles and the like. The reconstituted matrix can also be used for determining metastatic potential of tumor cells and for isolating metastatic tumor cells.
Type:
Grant
Filed:
May 27, 1986
Date of Patent:
May 9, 1989
Assignee:
The United States of America as represented by the Secretary of the Department of Health and Human Services
Abstract: Recombinant DNA molecules in which an avian retroviral long terminal repeat (LTR) has been ligated to the bovine growth hormone gene are described herein. The retroviral LTR was derived from a plasmid clone of a Schmidt Rupin strain of Rous Sarcoma Virus while the bovine growth hormone (BGH) gene was derived from a lambda-bacteriophage genomic library. Using a transient eucaryotic expression assay system, the plasmid constructs were screened for their ability to direct expression and secretion of bovine growth hormone. These constructs were co-transformed into a mammalian cell (mouse) culture in order to obtain a stable cell culture secreting large amounts of bovine growth hormone.
Type:
Grant
Filed:
May 14, 1984
Date of Patent:
May 9, 1989
Assignee:
Merck & Co., Inc.
Inventors:
John J. Kopchick, Frederick C. Leung, Thomas J. Livelli, Richard H. Malavarca
Abstract: The invention concerns the intercistronic DNA sequence of the atp operon of Escherichia coli for the subunit c of the ATP synthase, a DNA structure and an expression vector which are characterized by the said intercistronic DNA sequence, and a process for the production of proteins using the same. The DNA sequence enhances the rate of synthesis of the proteins.
Type:
Grant
Filed:
July 17, 1985
Date of Patent:
May 2, 1989
Assignee:
Gesellschaft fur Biotechnologische Forschung mbH
Abstract: This invention relates to a DNA base sequence, capable of increasing the amount of protein secreted by microorganisms, and its derivative sequences; a recombinant plasmid including the whole or a part of said DNA base sequence; a method for preparing the recombinant plasmid in which, when microorganisms having introduced thereinto a recombinant DNA including the desired DNA base sequence are to be separated in the process of cloning, suitable transformants can be efficiently selected by taking the amount of protein secreted out of the cells and particularly the activity of an enzyme protein as an index; and a method of microbial breeding which comprises introducing the recombinant plasmid into a microorganism to increase the amount of protein secreted by the microorganism.
Type:
Grant
Filed:
April 8, 1988
Date of Patent:
April 25, 1989
Assignees:
Agency of Industrial Science and Technology, Ministry of International Trade and Industry