Abstract: Described herein are compositions and methods of using modified placental tissue to achieve endogenous and exogenous therapeutic effects. When applied to an injured or diseased organ or body part, the modified placental tissue elicit stem cell recruitment and/or localization directly to or proximate to the site of application. Also described is a novel vacuum drying device and the use thereof.

Abstract: Sentinel Cells, respond quickly and differentially to and among various high explosives (e.g., RDX, PETN, HMX, TNT, TNG), taggant (DMDNB), and bacteria, as well as volatiles of commercial C4, Semtex 1A, and military C4. These effects are observable within 5 minutes of exposure, indicate profound biological effects in exposed cells, and are distinctly different from responses to bacteria (e.g., E. coli, mycoplasma, lipopolysaccharide from E. coli, etc.). Quantitative and mechanistic analysis of the observations indicate that the mechanisms that give rise to these responses invoke different subsets of biochemical reactions. The mechanism within the exposed Sentinel Cells is also used in canine olfactory cells (e.g., bomb dogs). The nature of the explosives-induced biological effects provide for a cell based explosives detection system (i.e., a cellular dog nose), as well as a tool for the study of biological effects of energetic materials on humans.

Abstract: Methods of ex-vivo culture of natural killer (NK) cells are provided and, more particularly, methods for enhancing propagation and/or functionality of NK cells by treating the cells with an aryl hydrocarbon antagonist in combination with cytokines driving NK cell proliferation. Also envisioned are compositions comprising cultured NK cells and therapeutic uses thereof.

Abstract: The Present Invention aims to diminish the effects of alcohol consumption by breaking down the “stacked” excess alcohol in the digestive track through its probiotic element which effectively “eats” away at the alcohol before the liver has to process it. It comprises a personal dose of Acetobacter aceti, which is a benign microorganism that is ubiquitous in the environment, existing in alcoholic ecological niches such as flowers, fruits, honey bees, as well as in water and soil.

Abstract: The disclosure provides methods for treating diabetes by promoting graft survival and improved graft function in a patient receiving an islet transplant by treating the patient with a GLP-1 receptor agonist compound prior to the islet transplant. The methods may also comprise treating the islets with a GLP-1 receptor agonist compound prior to transplanting them in a patient. The methods may eliminate the need for immunosuppressive therapy in islet transplants. Any GLP-1 receptor agonist compound know in the art can be used in the methods described herein.

Abstract: Red blood cell membrane derived microparticles (RMP) are safe, economical, effective hemostatic agents in the treatment of a wide range of bleeding conditions and can be considered as universal hemostatic agents. RMP are produced from red blood cells using a high-pressure extrusion membrane shear process and can be lyophilized after production and retain activity even when stored at room temperature. RMP can be administered to original donors (autologous treatment), thus avoiding transfusion complications, or can be administered to blood type compatible recipients. RMP produced from type O, Rh negative red cells can be given to any person regardless of blood type. RMP can be administered to reduce excessive bleeding resulting from trauma, surgeries, invasive procedures and various bleeding disorders such as platelet disorders, either congenital or acquired, and coagulation disorders, either congenital or acquired.

Abstract: The present invention relates to a method for the treatment of organs which are degenerative and/or in the pathological state by means of the use of cells, which are phenotypically stably differentiating or differentiated but not necessarily ultimately predetermined with respect to development, from a donor organ selected according to the principles of the opposite cell differentiation program (OCDP) and also to the use of cells of this type for the treatment or for the production of a drug for treatment of the same. Furthermore, the present invention relates to pharmaceutical agents comprising suitable phenotypically stable cells, cells of a first organ which is different from the second organ with respect to organ type thereby being used, which, in the normal physiological state with respect to a predetermined set of expressed genes and/or phenotypical properties, have opposite properties to the second cells in the normal physiological state.

Abstract: A method that enables quantification of cholesterol in high-density lipoprotein 3 (HDL3) in a test sample without requiring a laborious operation is disclosed. The method for quantifying cholesterol in HDL3 comprises reacting, with a test sample, a surfactant that specifically reacts with high-density lipoprotein 3, and quantifying cholesterol. The method enables specific quantification of HDL3 cholesterol in a test sample using an automatic analyzer without requirement of a laborious operation such as ultracentrifugation or pretreatment. Further, quantification of the HDL2 cholesterol level can also be carried out by subtracting the HDL3 cholesterol level from the total HDL cholesterol level obtained by a conventional method for quantifying the total HDL cholesterol in a test sample.

Abstract: The subject of the present invention is a process for producing adherent cells comprising: a. introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium; b. amplifying the cells by performing a plurality of cell passages in the same culture vessel wherein each cell passage subsequent to the first is carried out: i) by using all or part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from the microcarriers, and ii) by introducing culture medium and an increasing amount of microcarriers into the culture medium; and c. harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers. The invention also relates to the implementation of this process for the production of biological agents, serving in particular to prepare vaccines or drugs.

Abstract: A method for producing at least one compound selected from the group consisting of 1,8-cineole, and 1-methyl-1,4-cyclohexadiene is also described. The method includes culturing a microorganism on or within a culturing media in a container under conditions sufficient for producing the at least one compound.

Abstract: The present invention relates to methods for recellularization of blood vessels. This method is particularly useful for producing an allogeneic vein, wherein a donor vein is decellularized and then recellularized using whole blood or bone marrow stem cells. The allogeneic veins produced by the methods disclosed herein are particularly advantageous for implantation or transplantation into patients with vascular diseases.

Abstract: The present invention relates to a method for evaluating the improvement of skin elasticity by preparing a mimetic dermis having a structure similar to that of the real dermis, and providing a three-dimensional visualization of the differences in the degree of transformation of the mimetic dermis.

Abstract: Methods for isolating viable cancer cells from a sample that comprises a mixture of cancerous cells and normal (non-cancerous) cells are provided. In the methods, a fluid preparation comprising a mixture of cancerous and normal cells is repeatedly exposed to fluid shear stresses, whereby the repeated exposure to the fluid shear stresses preferentially imparts fluid shear stress-resistance to the cancerous cells.

Abstract: Systems, compositions, and methods for the bioremediation of a contaminant contained within a contaminated region. The systems, compositions, and methods may include supplying a first bioremediation formulation that includes an ion exchange resin to a first treatment zone that is associated with the contaminated region. The systems, compositions, and methods further may include supplying a second bioremediation formulation that includes a high-mobility oxidant, a low-mobility oxidant, and a nutrient material to a second treatment zone that is associated with the contaminated region. The systems, compositions, and methods also may include a kit of bioremediation formulations that includes the first bioremediation formulation and the second bioremediation formulation and is to be utilized during the bioremediation of the contaminated region.

Abstract: A functional microbially fermented tea extract containing a novel polyphenol derivative and a method for producing the same are provided. A functional microbially fermented tea extract containing various extracts and a novel polyphenol derivative is produced by subjecting, to an extraction treatment, a microbially fermented tea leaf obtained by culturing one species selected from Aspergillus sp. (PK-1), Aspergillus oryzae (NBRS 4214) sp. (AO-1), Aspergillus awamori (NBRS 4122) sp. (SK-1), and Eurotium sp. (KA-1) with a tea leaf. Furthermore, for example, a functional fermented tea extract enriched with a novel polyphenol derivative is produced.

Abstract: The present invention relates to a novel injectable delivery system for stem cell therapy, which comprises a thermo-sensitive amphiphlic chitosan nanogel. Therefore, the invention provides a method for repairing a tissue damage of a subject using the amphiphlic chitosan nanogel served as a carrier for delivering the stem cells to the damaged tissue. This invention also provides a method for sustaining the growth of stem cells using the amphiphlic chitosan nanogel served as a niche or scaffold.

Abstract: The present invention provides a preparing method of an allicin injection and the low temperature continuous stirring ultrafiltration device thereof. Said preparing method consists of the following steps: extracting allicin; diluting the allicin with solvent precooled to 1-4 in a clean environment, adding nitrogen gas or argon gas, and then encapsulating the solution to obtain allicin injection with different specifications.

Abstract: Inflammatory cytokines e.g. IFN-?, serve as initiating stimuli for mesenchymal stem cell (MSC) immunosuppresive activity in vivo. Other inflammatory cytokines, such as TNF alpha, the molecule hemoxygenase I, and TLR ligation of MSC, may also provide such a response. Activated MSC's promote tissue regeneration in conditions such as aging, where regeneration is impaired. Wound healing in aged mammals was enhanced by restoring tensile strength to the levels of younger mammals. Activated MSCs were useful in treating wounds in diabetic primates.

Abstract: The present invention relates to a microorganisms of the genus Corynebacterium producing 5?-inosinic acid, in which the expression of genes encoding purine biosynthesis related enzymes is increased higher than the intrinsic expression, and to a method for producing 5?-inosinic acid, comprising culturing the microorganisms of the genus Corynebacterium with improved 5?-inosinic acid productivity.

Abstract: The present invention relates to methods of in vitro preparation of a parental cell bank (PCB) from foetal tissue consisting of foetal epiphyseal tissue, foetal Achilles tendon tissue and foetal skin tissue, using a rapid mechanical primary cell culture selection of cell type to be used in methods for wound and tissue repair.