Abstract: A method for determining the presence and/or concentration of a water treatment polymer in an aqueous sample, comprising producing a polyclonal or monoclonal antibody to the water treatment polymer, and using the antibody so produced as a reagent in an immunoassay, conducted on the aqueous sample.

Abstract: In accordance with the method of the invention and within the framework of the split synthesis technique, compounds are synthesized on macroscopic solid support units which are spatially organized in groups, referred to herein as strings, so that the location of each unit in a string is known and recorded. Each string is placed in a reaction vessel for contact with a suitable reagent or reagents to couple a first component on the support units. Each string is submitted to a different reaction and the sequential arrangement of the support units is maintained throughout the whole reaction step and any subsequent washing steps. At the completion of a reaction step the support units of the strings (referred to as source strings) are sorted in a different sequential arrangement to form new strings (referred to as destination strings) for the next reaction step. The reorganization of the support units into new destination strings is recorded for each reaction step in the synthesis.

Abstract: Methods are provided for generating highly diverse libraries of expression vectors encoding fusion proteins such as single-chain antibodies via homologous recombination in yeast. The method comprises: transforming into yeast cells a linearized yeast expression vector having a 5′- and 3′-terminus sequence at the site of linearization and a library of insert nucleotide sequences that are linear and double-stranded; and having homologous recombination occur between the vector and the insert sequence such that the insert sequence is included in the vector in the transformed yeast cells.

Abstract: The present invention is directed to a strategy to discover small peptides that will activate any G protein coupled receptor (GPCR) or inactivate any constitutively active GPCR. The strategy uses combinatorial peptide libraries to screen millions of random peptides for agonist/negative antagonist activity. The method of the subject invention comprises expressing a peptide of a peptide library tethered to a G protein coupled receptor of interest in a cell, and monitoring the cell to determine whether the peptide is an agonist or negative antagonist of the GPCR of interest. The peptide is tethered to the GPCR by replacing the amino terminus of the GPCR with the amino terminus of a self-activating receptor, and replacing the natural peptide ligand present in the amino terminus of the self-activating receptor with the peptide of the peptide library.

Abstract: A method of fabricating an array of biopolymers provides a shield for biochemical reactions and biochemical reactants and is particularly useful for those reactions and reactants that are susceptible to reaction with a component of the ambient environment during the fabrication of the array. The method is applicable to the conventional fabrication and synthesis methods used to fabricate a biopolymer array, such as in situ synthesis of biopolymers on an array and the attachment of pre-synthesized biopolymers on to an array. The method comprises applying a non-miscible fluid (NMF) to the array surface where the biopolymers are being synthesized or attached. The NMF is inert and insoluble with the biochemical reactants and other ancillary materials in solution used in conventional synthesis or attachment of biopolymers.

Abstract: The invention provides a method for engineering and selecting an active protease able to cleave a user-defined target amino acid sequence. The method is useful for designing proteases for medical therapeutics and industrial applications.

Abstract: A method of monitoring the amount of primary or secondary ligand in a cell, using a green fluorescent protein complex and fluorescence resonance energy transfer between two GFP molecules to monitor the primary or secondary ligand, is disclosed. Further disclosed is a method of screening a molecule, such as a peptide, for primary ligand-binding activity, which also uses a green fluorescent protein complex and fluorescence resonance energy transfer between two GFP molecules, to screen the molecule or peptide. A green fluorescent protein complex having a first green fluorescent protein, a primary ligand-binding peptide, and a second green fluorescent protein, is also disclosed.

Abstract: Methods and compositions are provided for producing arrays of polymeric binding agents. In the subject methods, the individual polymers of the array are synthesized using solid phase synthesis techniques on the surface of a substrate. A critical feature of the invention is that one or more locations on the substrate surface are spatially and temporally protected by a protective bubble during the synthesis protocol, where the protective bubble may be produced using any convenient bubble producing means. The bubble producing means may be a component of either a substrate or a structure separate from the substrate. Also provided are the arrays produced by the subject methods, kits for use in practicing the subject methods, and methods of using the arrays in analyte detection assays, including hybridization assays, such as gene discovery, differential gene expression and gene sequencing assays.

Abstract: Combinations of a cyclooxygenase-2 inhibitor and a leukotriene B4 receptor antagonist are described for treatment of inflammation and inflammation-related disorders.

Abstract: The present invention provides a method for treating pain comprising administering an analgesic dosage of olanzapine to an animal in need of such treatment.