Abstract: Provided is a methylated tumor marker STAMP-EP2 and uses thereof in preparing a tumor diagnostic reagen. The tumor marker STAMP-EP2 herein is hypermethylated in all tumor types, is hypomethylated in corresponding normal tissue, and has high sensitivity and specificity; primers for detecting STAMP-EP2 may be used to prepare a tumor diagnostic kit.

Abstract: Disclosed herein are methods, kits and compositions for treating an inflammatory disease. These methods. kits and compositions may be particularly useful for subjects carrying a risk genotype and/or expressing a transcriptomic risk signature that is indicative of severe inflammatory disease phenotypes for which existing treatment options are limited.

Abstract: A high throughput method for detecting chromosomal aberrations and/or telomere aberrations using a biological sample of 150 ?L to 200 ?L including preparing a cytogenetic slide from the sample in a microplate, the mitotic index in the cytogenetic slide being 3 times higher on average than the conventional procedure of culturing cells in flasks with 10 to 20 mL of medium, simultaneously labeling the telomeres and centromeres with peptide nucleic acid probes with a hybridisation time from 30 minutes to 1.5 hours, flow image quantifying the fluorescence intensity of telomeres on interphase nuclei using a 10× magnification objective for overall telomere quantification, and automatically capturing the metaphase chromosomes to detect chromosomal aberrations and/or telomere aberrations in each chromosome. Also a high-throughput detection kit for quantifying telomeres and detecting chromosomal aberrations and/or telomere aberrations.

Abstract: A method of collecting oral mucosal cells from a patient and evaluating the oral mucosal cells for oral squamous cell carcinoma predisposition in the patient includes collecting a sample of chewing gum from a patient that had chewed the chewing gum for a minimum of about 5 minutes up to about 30 minutes. The chewing gum includes a molecular adherent targeted towards oral mucosal cells with oral squamous cell carcinoma predisposition. A polymerase chain reaction (PCR) is performed on a DNA template prepared from the chewed chewing gum using oligonucleotide primers that are selected to amplify nucleic acids that screen for genomic sequence variations seen in genes that are indicative of oral squamous cell carcinoma predisposition in the patient.

Abstract: The invention is related to an inducible promoter for improved and regulated gene expression, useful in synthetic biology and metabolic engineering. In particular, the present invention relates to a nucleotide sequence comprising the regulatory regions of an erythritol- and erythrulose-inducible promoter in yeast and uses thereof in an expression system thus allowing an improved and regulated gene expression and production of gene product.

Abstract: In some embodiments described herein are methods for three-dimensional analysis of a biological sample, comprising migrating a population of spatial probes into the biological sample in each of three dimensions, wherein each spatial probe comprises a targeting domain and a migration domain. Also provided are kits and compositions for use according to any of the methods described herein.

Abstract: Provided are methods and systems for metagenomic analysis. Methods involve generating libraries and databases of taxonomic signatures informative of taxonomic information in a heterogeneous microbial sample. Methods also include identification of microorganisms and biochemical activities in the sample, including identification at a plurality of taxonomic levels.

Abstract: The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, the methods involve the use of a set of probe polynucleotides, for example a set of three or more probe polynucleotides, for assessing target nucleic acids. In some aspects, the presence, amount, and/or identity of region of interest in a target nucleic acid is analyzed in situ. Also provided are polynucleotides, sets of polynucleotides, compositions, and kits for use in accordance with the methods.

Abstract: The present disclosure belongs to the field of livestock molecular biotechnology, and provides kits and methods for pedigree division and paternity testing of domestic pigs. The kits and methods specifically select 14 SSR loci of domestic pigs, especially Anqing six-end-white pigs, and synthesize primers for corresponding loci. Through capillary electrophoresis detection of the ear tissue DNA of 98 Anqing six-end-white pigs, the count of effective alleles, heterozygosity, polymorphism information content, and genetic distance, and exclusion probability at each locus are calculated, the pedigree division of domestic pigs, especially Anqing six-end-white pigs, and paternity testing are conducted. The cumulative exclusion probability of 14 microsatellite loci is 99%.

Abstract: Methods, devices and kits for sampling, releasing and stabilizing nucleic acid, including RNA and DNA, from virus, bacteria yeast and other cells is described. The released and stabilized nucleic acid may be analyzed and quantified without further sample preparation at the point of care or may be transported to a testing laboratory by shipment and analyzed directly. The nucleic acid, which can be RNA, remains safe and stable so that shipping by normal means including government postal service may be used. In addition, the RNA sample remains stable so that analysis can be performed immediately after receipt of sample or after storage for days, weeks or months. Storage may be at room or ambient temperature or cooler temperatures. The sampling apparatus used to acquire samples can interface with nucleic detection and measurement instrumentation including high throughput, parallel processing instruments.