Abstract: An object to be achieved by the present invention is to prepare a droplet using a protein that has not been known heretofore to form a droplet. The present invention provides a droplet including an assembly of a protein, wherein the protein is a PDI family protein and/or an artificial protein, and contains a thioredoxin-like domain.

Abstract: Unnatural, mutant tryptophan decarboxylase proteins and methods of using same. The mutants have an amino acid sequence at least 80% identical to SEQ ID NO:1 and have substitutions at one or more of amino acid positions 98, 99, 120, 126, 339, 349, and 355 of SEQ ID NO:1. The mutants can have enhanced activity and/or substrate promiscuity and be used for synthesizing tryptamine analogs.

Abstract: Described herein are devices and methods for increasing the production of steviol glycosides, which have industrial and economic value. The steviol glycosides produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods and do not have a bitter aftertaste, making them better suited as flavor-enhancing additives to food, pharmaceutical, and nutritional supplement products.

Abstract: Provided is a method of producing an L-amino acid such as L-glutamic acid and the like. An L-amino-acid is produced by cultivating a coryneform bacterium having L-amino acid-producing ability in a culture medium, which has been modified so as to have one or more of the following modifications: (A) a modification for increasing activity of acetate kinase, (B) a modification for increasing activity of fructose-1,6-bisphosphatase, (C) a modification for decreasing activity of pyruvate dehydrogenase, (D) a modification for decreasing activity of aspartate transaminase, and (E) a modification for decreasing activity of malic enzyme; and collecting the L-amino acid from the culture medium and/or the bacterial cells.

Abstract: The present invention relates to a method for the recombinant production of olivetolic acid (OA) in a host species selected from amoebozoa, based on a hybrid-gene or enzyme of polyketide synthase 37 (PKS37) in which the C-terminal type III PKS domain from an amoeba is replaced by an olivetol synthase (OLS) from a plant, and is expressed together with an olivetolic acid cyclase from a multi-gene expression vector. Further provided is a recombinant amoebozoa host species, and an improved method for producing ?9-tetrahydrocannabinol (THC) or other cannabinoids.

Abstract: The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.

Abstract: A method for site-selective deuteration of amino acids using a protein system having an aminotransferase (e.g., DsaD) and/or a small partner protein (e.g., DsaE). A non-deuterated amino acid is contacted with deuterium and an aminotransferase or a combination of an aminotransferase and a partner protein, to yield a C?-deuterated or a C?- and C?-deuterated amino acid. C?-deuterated amino acids can be accessed by contacting a C?- and C?-deuterated amino acid with non-deuterium hydrogen and an aminotransferase to wash out the deuterium at the C? carbon atom by the non-deuterium hydrogen.

Abstract: The instant application provides methods to improve the total starch yield and/or gluten yield from corn kernels in a wet milling process, the method comprising admixing corn kernels or a fraction of the corn kernels with an enzyme composition comprising an effective amount of one or more hydrolytic enzymes, wherein at least one of said hydrolytic enzymes is selected from the group consisting of a GH30 polypeptide, a GH5 polypeptide or a combination thereof.

Abstract: Industrial fermentation for the production of lactic acid from organic waste combined with chemical recycling of polylactic acid are provided, to obtain lactic acid at high yields.

Abstract: The present invention relates to animal feed and animal feed additives comprising polypeptides having protease activity, as well as methods of producing proteases, methods of producing animal feed and animal feed additives comprising proteases, and methods of using proteases to improve animal performance and the nutritional value of animal feed.

Abstract: Recombinant cells and methods for producing methyl ketones, such as medium-chain methyl ketones. The recombinant cells include recombinant acyl-ACP thioesterase genes, recombinant ?-ketoacyl-CoA thioesterase genes, and recombinant acyl-CoA synthetase genes, in addition to other modifications. The methods include culturing the recombinant cells to produce the methyl ketones and isolating the produced methyl ketones.

Abstract: Provided are a phytase mutant and a coding DNA molecule thereof, a vector, and a host cell. The phytase mutant comprises an amino acid sequence having at least 90% identity with SEQ ID NO: 3, and compared with SEQ ID NO: 3, and contains an amino acid substitution at at least one position selected from the group consisting of 36, 126, 211, 253, 258, and 266. The heat resistance of the mutant is significantly improved, thus facilitating the wide application of phytase in feed.

Abstract: Aerobic microbial processes comprise culturing microbes comprising a (per)chlorate respiration pathway in a bioreactor in a bioprocess employing microbial respiration of chloroxyanions as a source of oxygen for the bioprocess, in the absence of external addition of molecular oxygen.

Abstract: Provided are subtilase variants having improved stability, detergent compositions comprising the variants, use of the variants and detergent compositions in a cleaning process such as laundry or hard surface cleaning such as dishwashing, and methods of producing the variants.

Abstract: The present invention relates to a genetically engineered microorganism expressing (i) formate tetrahydrofolate (THF) ligase, methenyi-THF cyclohydrolase and methylene-THF dehydrogenase, (ii) the enzymes of the glycine cleavage system (GCS), (iii) serine deaminase and serine hydroxymethyltransferase (SHMT), (iv) an enzyme increasing the availability of NADPH, and (v) optionally formate dehydrogenase (FDH), and wherein the genetically engineered microorganism has been genetically engineered to express at least one of the enzymes of (i) to (v), wheren said enzyme is not expressed by the corresponding microorganism that has been used to prepare the genetically engineered microorganism, and wherein the enzymes of (i) to (v) are genomically expressed.

Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

Abstract: The present invention relates to enzymes having DNA polymerase and 3?-5? exonuclease activities. In particular, the present invention relates to a heat labile enzyme possessing a DNA polymerase II activity and a 3?-5? exonuclease activity of marine origin. Furthermore, the present invention relates to a DNA polymerase primarily exerting a 3?-5? activity, i.e. where the polymerase activity is absent. The present invention furthermore relates to the use of the exonuclease activity to degrade the 3?-5? strand of double stranded DNA to perform single stranded overhang, e.g. in recombinant cloning processes, or in processes for removal of contaminating nucleic acid molecules.

Abstract: Disclosed are variants of a glycoside hydrolase having improved stability, e.g., in the presence of a protease, and the use of such variants in detergent applications, such as laundry or dish wash.

Abstract: Methods and engineered hosts are disclosed that convert a lignocellulosic xylose-containing biomass source into xylonic acid and/or xylonate, which can be further processed into other useful derivatives. In particular, an exemplary engineered Bacidiomycetes, e.g., R. toruloides, host produces/expresses one or more fungal enzymes that convert xylose into xylonic acid/xylonate. Methods of using such hosts to consume pretreated lignocellulosic biomass in combination with certain native promoters and heterologous genes are also described herein.

Abstract: A genetically modified microorganism that can produce 3-hydroxyadipic acid and/or ?-hydromuconic acid with a high yield; and a method of producing 3-hydroxyadipic acid and/or ?-hydromuconic acid using the genetically modified microorganism, are disclosed. The genetically modified microorganism has an ability to produce 3-hydroxyadipic acid and/or ?-hydromuconic acid, and has an enhanced enzymatic activity to catalyze a reaction to reduce 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, wherein, in the genetically modified microorganism, a dicarboxylic acid excretion carrier function is deleted or decreased.