Patents Examined by Todd M Epstein
  • Patent number: 11518989
    Abstract: A method of improving a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) to have a higher protein score is disclosed. The method includes the steps of: making a modified RbcL of the RuBisCo, by, on an RbcL unit of the RuBisCo, either substituting Met for Leu, Phe, Val, or Ile or combinations thereof; substituting Lys for Arg, Thr, or His or combinations thereof; or both of these substitutions. The modified RbcL consequently modifies the RuBisCo and is added to a biomass host where it is stable for homologous recombination. Plastid and nucleus integration was observed. Example RbcL sequences are disclosed with the desirable substitutions. The improved RuBisCo can be used as an improved proteinaceous food source for humans and animals.
    Type: Grant
    Filed: September 30, 2020
    Date of Patent: December 6, 2022
    Assignees: National Technology & Engineering Solutions of Sandia, LLC, Arizona Board of Regents on behalf of Arizona State University
    Inventors: Ryan Wesley Davis, Joseph S. Schoeniger, Arul M. Varman, Muhammad Faisal, Aditya Pandharinath Sarnaik
  • Patent number: 11518987
    Abstract: The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: December 6, 2022
    Assignee: NOVOZYMES A/S
    Inventors: Jens Nielsen, Pernille Hede, Esben Peter Friis
  • Patent number: 11512299
    Abstract: The present invention has a purpose of providing a novel ?-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a ?-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.
    Type: Grant
    Filed: December 27, 2016
    Date of Patent: November 29, 2022
    Assignee: AMANO ENZYME INC.
    Inventors: Yukiko Hoshi, Masamichi Okada, Akio Horii, Masayuki Hojo
  • Patent number: 11499172
    Abstract: Use of a stereoselective transaminase in the asymmetric synthesis of a chiral amine. In particular, provided is use of a polypeptide in the production of a chiral amine or a downstream product using a chiral amine as a precursor. Further provided is a method for producing a chiral amine, comprising culturing a strain expressing the polypeptide so as to obtain a chiral amine. Further provided are a chiral amine production strain and a method for constructing the chiral amine production strain. The stereoselective transaminase has a broad substrate spectrum and thus has a broad application potential in the preparation of a chiral amine.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: November 15, 2022
    Assignee: Abiochem Biotechnology Co., Ltd.
    Inventors: Zhanbing Cheng, Tao Zhang, Zhenhua Tian, Shaonan Ding
  • Patent number: 11479763
    Abstract: Certain embodiments of the disclosure are directed to the expression/production of ?-galactosidases in recombinant Bacillus spp. host cells having reduced or eliminated para-nitrobenzylesterase activity.
    Type: Grant
    Filed: April 5, 2018
    Date of Patent: October 25, 2022
    Assignee: Dupont Nutrition Biosciences APS
    Inventors: Cristina Bongiorni, Karina Hansen Kjaer
  • Patent number: 11459589
    Abstract: The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.
    Type: Grant
    Filed: November 11, 2020
    Date of Patent: October 4, 2022
    Assignee: LanzaTech NZ, Inc.
    Inventor: Michael Koepke
  • Patent number: 11453895
    Abstract: The present invention relates to methods and engineered microbial hosts useful for treating lignin or a derivative thereof. In some embodiments, the host has one or more exogenous nucleic acid sequences that encode a ligninase (e.g., a laccase and/or a peroxidase).
    Type: Grant
    Filed: April 2, 2020
    Date of Patent: September 27, 2022
    Assignee: National Technology & Engineering Solutions of Sandia, LLC
    Inventors: Seema Singh, Arul Mozhy Varman
  • Patent number: 11447800
    Abstract: The present disclosure relates to a novel method of producing vanillin and/or derivatives thereof by applying improved biocatalysts. Also provided herein are expression systems for preparing said improved biocatalysts. Moreover provided herewith are novel enzyme mutants, corresponding coding sequences and vectors applicable in the biochemical production of vanillin. The present disclosure further provides recombinant host cells or organisms genetically modified for improved functional expression of biocatalysts, as well as recombinant host cells or organisms useful to produce vanillin.
    Type: Grant
    Filed: March 29, 2019
    Date of Patent: September 20, 2022
    Assignee: FIRMENICH SA
    Inventor: Fredi Bruhlmann
  • Patent number: 11441129
    Abstract: The present invention provides at least two modified gene sequences, Sequence 1 comprising of nucleotide sequence of SEQ. ID no.1, and Sequence 2 comprising of nucleotide sequence of SEQ. ID no.2, encoding the enzyme choline oxidase wherein the gene sequences have been obtained by modifying the codA gene (Accession no. X84895) encoding choline oxidase from Arthrobacter globiformis, and a method to enzymatically produce betaine using choline oxidases encoded by Sequence 1, and Sequence 2, wherein the enzymatically produced betaine has minimal undesired trimethylamine contamination.
    Type: Grant
    Filed: July 31, 2018
    Date of Patent: September 13, 2022
    Inventor: Prashant Gaur
  • Patent number: 11414683
    Abstract: The invention describes a process for the production of ethanol from a composition comprising glucose and between 50 ?M and 100 mM acetic acid, said process comprising fermenting said composition in the presence of a recombinant yeast which is capable to convert acetic acid anaerobically; maintaining the amount of undissociated acetic acid at a value of at least 50 ?M; and recovering the ethanol. Said process is useful for both starch and cellulosic based, acetic acid containing hydrolysates and advantageously results in a greater consumption of acetic acid and thus higher ethanol yields.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: August 16, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventor: Hans Marinus Charles Johannes De Bruijn
  • Patent number: 11407989
    Abstract: The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: October 29, 2015
    Date of Patent: August 9, 2022
    Assignee: Novozymes A/S
    Inventors: Annette H. Johansen, Rolf T. Lenhard, Peter Esben Friis
  • Patent number: 11390856
    Abstract: The present invention relates to variant of Family A polymerases able to synthesize a nucleic acid fragment without template and to incorporate a reversible modified terminator nucleotide during the nucleic acid fragment synthesis. The present invention further relates to uses thereof for enzymatic synthesis of nucleic acid molecules.
    Type: Grant
    Filed: August 6, 2018
    Date of Patent: July 19, 2022
    Assignee: DNA Script
    Inventors: Thomas Ybert, Elise Champion, Omar Vivar, Ahmed Said
  • Patent number: 11390857
    Abstract: The present invention provides a DNA polymerase including the sequence of SEQ ID NO. 1 or a sequence which is at least 70% identical thereto, but wherein the aspartic acid residue at position 18 of SEQ ID NO. 1, or the equivalent aspartic acid residue in other sequences, has been replaced by a non-negatively charged amino acid residue. It further provides DNA polymerases comprising the amino acid sequences of SEQ ID NO. 2, 11 and 12 and variants thereof. The present invention also provides nucleic acids encoding the DNA polymerases, a method of producing said DNA polymerases, and compositions, expression vectors and host cells or viruses comprising said DNA polymerases. The present invention also provides uses of said DNA polymerases in nucleotide polymerisation, amplification and sequencing reactions.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: July 19, 2022
    Assignee: UNIVERSITETET I TROMSØ—NORGES ARTISKE UNIVERSITET
    Inventors: Atle Noralf Larsen, Yvonne Piotrowski
  • Patent number: 11384368
    Abstract: The invention relates to a recombinant yeast comprising a recombinant yeast comprising a nucleotide sequence coding for a glycerol dehydrogenase, a nucleotide sequence coding for a ribulose-1,5-biphosphate carboxylase oxygenase (EC 4.1.1.39); a nucleotide sequence coding for a phosphoribulokinasey (EC 2.7.1.19); a nucleotide sequence allowing the expression of a glucoamylase (EC 3.2.1.20 or 3.2.1.3); and optionally a nucleotide sequence coding for a glycerol transporter. This cell can be used for the production of ethanol and advantageously produces little or no glycerol.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: July 12, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paulus Petrus De Waal, Ingrid Maria Vugt-Van Lutz
  • Patent number: 11384370
    Abstract: The present disclosure relates to a microorganism for producing a mycosporine-like amino acid, and a method for producing a mycosporine-like amino acid using the microorganism. The microorganism of the present disclosure shows an improved ability for producing a mycosporine-like amino acid and thus can be effectively used in the production of a mycosporine-like amino acid.
    Type: Grant
    Filed: August 13, 2018
    Date of Patent: July 12, 2022
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Sol Kim, Kyusung Lee, Joo Hee Lee, Jong-cheol Seok, Jae Woo Jang
  • Patent number: 11377650
    Abstract: The present invention relates to isolated polypeptides having D-psicose 3-epimerase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: August 30, 2018
    Date of Patent: July 5, 2022
    Assignee: Novozymes A/S
    Inventors: Xinyu Shen, Randall Scott Deinhammer, James Ron Huffman, Kendra Stallings, Tine Hoff, Jesper Salomon, Anne Goldbech Olsen
  • Patent number: 11370815
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.
    Type: Grant
    Filed: December 30, 2020
    Date of Patent: June 28, 2022
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce
  • Patent number: 11365417
    Abstract: Described herein are devices and methods for increasing the production of steviol glycosides, which have industrial and economic value. The steviol glycosides produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods and do not have a bitter aftertaste, making them better suited as flavor-enhancing additives to food, pharmaceutical, and nutritional supplement products.
    Type: Grant
    Filed: September 10, 2018
    Date of Patent: June 21, 2022
    Assignee: Bio Capital Holdings, LLC
    Inventor: Raul Cuero Rengifo
  • Patent number: 11352650
    Abstract: The invention provides a genetically modified micro-organism for intracellular biosynthesis of a cellular metabolite, comprising a synthetic error correction system having a penalty gene, whose expression leads to arrested growth or cell death (e.g. a toxin gene) in combination with a survival gene, whose expression provides an antidote that restores cell viability and normal growth (e.g. a cognate antitoxin gene). Alternatively, the system has a survival gene, alone, whose expression is essential for growth (i.e. essential gene). The synthetic error correction system further comprises a biosensor, whose function is to induce expression of the survival gene which leads to cell growth, only, when the cell produces a pre-defined level of a given metabolite.
    Type: Grant
    Filed: September 23, 2016
    Date of Patent: June 7, 2022
    Assignee: DANMARKS TEKNISKE UNIVERSITET
    Inventors: Peter Rugbjerg, Kira Sarup-Lytzen, Morten Sommer
  • Patent number: 11339414
    Abstract: The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.
    Type: Grant
    Filed: March 2, 2020
    Date of Patent: May 24, 2022
    Assignee: BRASKEM S.A.
    Inventors: Paulo Moises Raduan Alexandrino, Iuri Estrada Gouvea, Veronica Leite Queiroz