Patents Examined by Todd M Epstein
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Patent number: 11976314Abstract: The present invention relates to a transformed strain having ethanol production potential, constructed by introducing a foreign gene for ethanol production into a non-ethanol producing acetogen Eubacterium limosum and a method for producing ethanol, using the strain. According to the present invention, Eubacterium limosum which is a conventional acetogen lacking ethanol production potential is used to produce ethanol, which is a high value-added product, as a single product from carbon monoxide contained in waste gas.Type: GrantFiled: August 28, 2018Date of Patent: May 7, 2024Assignees: GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY, KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATIONInventors: In Seop Chang, Jiyeong Jeong, Shinyoung Park, In-Geol Choi, Byeonghyeok Park
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Patent number: 11965189Abstract: The present invention relates to cellobiohydrolase variants having increased thermal activity or thermostability, polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: March 24, 2017Date of Patent: April 23, 2024Assignee: Novozymes A/SInventors: Mark Wogulis, Leslie DeMars, David Osborn
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Patent number: 11959105Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.Type: GrantFiled: June 26, 2020Date of Patent: April 16, 2024Assignee: Quantum-Si IncorporatedInventors: Brian Reed, Manjula Pandey
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Patent number: 11952609Abstract: A method of producing succinic acid from a biomass. The method comprises adding citric acid to a biomass comprising microalgae to form an acidified microalgae composition. The acidified microalgae biomass composition is stored under anaerobic conditions without inoculating the acidified microalgae composition with bacteria formulated to produce succinic acid. A coproduct comprising succinic acid is produced. Other methods of producing succinic acid from a biomass are also disclosed.Type: GrantFiled: February 28, 2022Date of Patent: April 9, 2024Assignee: Battelle Energy Alliance, LLCInventors: Lynn M. Wendt, Bradley D. Wahlen, Birendra Adhikari
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Patent number: 11939583Abstract: The present invention relates to autotrophic microorganisms with altered photorespiration and improved CO2 fixation as well as a method of producing said autotrophic microorganisms. Particularly, the autotrophic microorganisms show an improved growth rate, productivity and energy conversion efficiency.Type: GrantFiled: April 15, 2019Date of Patent: March 26, 2024Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.Inventors: Lennart Schada Von Borzyskowski, Tobias Jürgen Erb, Jan Zarzycki
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Patent number: 11932897Abstract: A method of making curcuminoids in a mammalian cell. The method of making a curcuminoid in a mammalian cell includes expressing one or more enzymes in the mammalian cell, the enzymes being selected from the group consisting of tyrosine ammonia lyase (TAL), 4-coumaroyl-CoA ligase (4CL1), curcuminoid synthase (CUS), diketide-CoA synthase (DCS), curcumin synthase (CURS1), 4-coumarate 3-hydroxylase (C3H), caffeoyl-CoA 3-O-methyltransferase (CCoAMT), and acetyl-CoA carboxylase (ACC). The expressing of the one or more enzymes converts a starting material, such as tyrosine or ferulic acid, to the curcuminoid. Also provided herein are therapeutic uses for the curcuminoid made in a mammalian cell.Type: GrantFiled: December 18, 2019Date of Patent: March 19, 2024Assignee: University of Kentucky Research FoundationInventors: Logan W. Warriner, Daniel W. Pack
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Patent number: 11926852Abstract: The present invention relates to compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity for use in, e.g., animal feed. The present invention further relates to polypeptides having arabinofuranosidase activity, polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: May 28, 2021Date of Patent: March 12, 2024Assignee: Novozymes A/SInventors: Wei Peng, Ninfa Rangel Pedersen, Dan Pettersson, Jens Magnus Eklof, Soren Nymand-Grarup, Lorena G. Palmen, Rune Nygaard Monrad, Nikolaj Spodsberg, Mary Ann Stringer, Charlotte Blom, Lars Kiemer, Kristian Bertel Romer M. Krogh, Jesper Salomon
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Patent number: 11926856Abstract: The present invention relates to isolated polypeptides having D-psicose 3-epimerase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: June 2, 2022Date of Patent: March 12, 2024Assignee: Novozymes A/SInventors: Xinyu Shen, Randall Scott Deinhammer, James Ron Huffman, Kendra Stallings, Tine Hoff, Jesper Salomon, Anne Goldbech Olsen
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Patent number: 11926851Abstract: The present invention relates to esterases, more particularly to esterase variants having improved activity and/or improved thermostability compared to the esterase of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.Type: GrantFiled: December 22, 2022Date of Patent: March 12, 2024Assignee: CARBIOSInventors: Benoît David, Isabelle Andre, Maher Ben Khaled, Sophie Duquesne, Alain Marty
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Patent number: 11913056Abstract: Provided is engineered microorganisms expressing acetoacetyl-CoA reductase variants and a method for improving the yield of PHA. Compared with the wild-type acetoacetyl-CoA reductase represented by SEQ ID NO. 31, the variant has one or more of the following mutations: (1) mutation of valine at position 141 to isoleucine or leucine; (2) mutation of methionine at position 12 to threonine, serine, alanine, leucine, lysine or isoleucine; (3) mutation of isoleucine at position 194 to valine, leucine or methionine; (4) mutation of glutamic acid at position 42 to lysine, glutamine, leucine, aspartic acid, proline, threonine, asparagine, or histidine; and (5) mutation of phenylalanine at position 55 to valine, alanine or isoleucine. The variants and their coding genes can promote the synthesis and accumulation of PHA by the strain and increase the yield of PHA.Type: GrantFiled: June 28, 2022Date of Patent: February 27, 2024Assignee: SHENZHEN BLUEPHA BIOSCIENCES CO., LTD.Inventors: Jin Yin, Yu Wang, Xiaocui Xie, Huayu Zhang, Yakun Wu, Tian Liu, Pingan Tang
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Patent number: 11913037Abstract: The present invention provides engineered adenylate kinase (AdK) enzymes, polypeptides having AdK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing AdK enzymes are also provided. The present invention further provides compositions comprising the AdK enzymes and methods of using the engineered AdK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.Type: GrantFiled: April 1, 2022Date of Patent: February 27, 2024Assignee: Codexis, Inc.Inventors: Da Duan, Auric Anthony Sowell-Kantz, Aksiniya Lyubenova Petkova, Oscar Alvizo
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Patent number: 11898188Abstract: The present disclosure relates to a mutant microorganism in which a glycerol catabolic pathway and a 1,3-PDO biosynthetic pathway are introduced into a microorganism incapable of using glycerol as a carbon source, and a method of producing 1,3-PDO using the same. According to the present disclosure, it is possible to produce 1,3-PDO while growing a mutant microorganism having 1,3-PDO production ability by using the inexpensive raw material glycerol as a single carbon source. Thus, the present disclosure is useful for the economical production of 1,3-PDO.Type: GrantFiled: January 15, 2019Date of Patent: February 13, 2024Assignees: HANWHA SOLUTIONS CORPORATION, KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sang Yup Lee, Jae Sung Cho, Je Woong Kim, Yoo Sung Ko, Cindy Pricilia Surya Prabowo, Taehee Han, Euiduk Kim, Jae Won Choi, Changhee Cho, Jun Kyu Lee
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Patent number: 11879143Abstract: This disclosure relates to modified tryptophan synthase and more particularly to modified beta-subunits of tryptophan synthase. The disclosure further relates to cells expressing such modified subunits and methods of producing non-canonical amino acids.Type: GrantFiled: November 21, 2019Date of Patent: January 23, 2024Assignee: California Institute of TechnologyInventors: Andrew Richard Buller, Sabine Brinkmann-Chen, Michael Herger, David K. Romney, Javier Murciano Calles, Paul van Roye
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Patent number: 11873515Abstract: The present invention provides a dehydrogenase mutant L283V/L286V, and a preparation method and use thereof, and relates to the field of biomedicine technologies. An amino acid sequence of the mutant L283V/L286V is as shown in SEQ ID NO: 1; and the mutant is prepared by simultaneously mutating 283rd and 286th leucine of a dehydrogenase with an amino acid sequence as shown in SEQ ID NO: 3 into valine. The dehydrogenase mutant L283V/L286V shows high selectivity in catalyzing myosmine reduction reaction in a whole cell system to produce S-nornicotine, and has relatively high dehydrogenase and imine reductase activities, a short enzyme reduction time, and a high transformation rate. The product S-nornicotine obtained through the reaction has extremely high optical purity, which reduces the operation difficulty of subsequent purification.Type: GrantFiled: November 18, 2022Date of Patent: January 16, 2024Assignees: GOLDEN HEALTH (GUANGDONG) BIOTECHNOLOGY CO., LTD., TF BIOSYN BIOTECHNOLOGY CO., LTD.Inventors: Jiajun Huang, Rongxu Li, Ziying Wu, Haoxuan Hu, Shaoyu Bai, David Zhou, Tony Lu
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Patent number: 11866757Abstract: Methods using plasma-driven generation of H2O2 in an aqueous liquid may provide a substrate to enzymes, which are then capable to oxidize or hydroxylate organic compounds. A plasma device may produce an aqueous liquid comprising H2O2 for use in an enzymatic reaction.Type: GrantFiled: June 11, 2019Date of Patent: January 9, 2024Assignee: Ruhr-Universitaet BochumInventors: Julia Bandow, Abdulkadir Yayci, Marco Krewing, Robert Kourist, Alvaro Gomez Baraibar
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Patent number: 11866759Abstract: The present application relates to a technical field of Lactobacillus strains, specifically, to a method of producing calcium propionate by using Lactobacillus reuteri. The method is that: the Lactobacillus reuteri with inactivated alcohol dehydrogenase and 1,2-propanediol are mixed, then grown and reproduced, and then an enrichment culture is conducted; and, after enrichment culture, a strain is placed into a culture medium containing calcium ion for a fermentation culture, then calcium propionate is obtained.Type: GrantFiled: April 19, 2023Date of Patent: January 9, 2024Assignee: SHENZHEN AGRECOE BIOTECHNOLOGY CO., LTD.Inventors: Jianying Wang, Zongyao Kong
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Patent number: 11859222Abstract: The present invention has a purpose of providing a novel ?-galactosidase enzyme useful for the production of oligosaccharides. Disclosed is a ?-galactosidase enzyme comprising the amino acid sequence of any one of SEQ ID NOs: 1 to 4 or an amino acid sequence that is 80% or more identical to said amino acid sequence.Type: GrantFiled: October 7, 2022Date of Patent: January 2, 2024Assignee: AMANO ENZYME INC.Inventors: Yukiko Hoshi, Masamichi Okada, Akio Horii, Masayuki Hojo
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Patent number: 11851686Abstract: Improved soluble methane monooxygenases and soluble methane monooxygenase systems are provided.Type: GrantFiled: August 30, 2021Date of Patent: December 26, 2023Assignee: Industrial Microbes, Inc.Inventors: Elizabeth Jane Clarke, Derek Lorin Greenfield, Noah Charles Helman, Stephanie Rhianon Jones, Baolong Zhu
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Patent number: 11788079Abstract: The present invention relates to compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity for use in, e.g., animal feed. The present invention further relates to polypeptides having arabinofuranosidase activity, polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: February 17, 2022Date of Patent: October 17, 2023Assignee: Novozymes A/SInventors: Ninfa Rangel Pedersen, Dan Pettersson, Jens Magnus Eklof, Soeren Nymand-Grarup, Lorena Gonzalez Palmen, Rune Nygaard Monrad, Wei Peng, Nikolaj Spodsberg, Mary Ann Stringer, Charlotte Blom, Lars Kiemer, Kristian Bertel Romer M. Krogh, Jesper Salomon
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Patent number: 11781117Abstract: Disclosed are a machine learning gene mining method and a phosphinothricin dehydrogenase mutant for amino translocation. The phosphinothricin dehydrogenase mutant for amino translocation is obtained by mutation of a wild-type phosphinothricin dehydrogenase with an amino acid sequence as shown in SEQ ID No.2 at one of the following sites: (1) E263D-K134R-H96A-R290V; (2) E263D-K134R-H96A; (3) E263D-K134R; (4) E263D; (5) E263N; (6) E263C; and (7) E263G. The present invention utilizes the site-saturation mutagenesis technology to mutate a phosphinothricin dehydrogenase gene as shown in SEQ ID No. 1, finds that the 263rd, 134th, 290th and 290th positions are the key sites affecting enzyme activity and stereoselectivity, and obtains a mutant with enzyme activity and ee value much higher than those of the parent phosphinothricin dehydrogenase.Type: GrantFiled: October 20, 2021Date of Patent: October 10, 2023Assignee: ZHEJIANG UNIVERSITY OF TECHNOLOGYInventors: Yaping Xue, Feng Cheng, Dongyang Wu, Shuping Zou, Jianmiao Xu, Yuguo Zheng