Patents Examined by Todd M Epstein
  • Patent number: 11015178
    Abstract: The present invention is related to a method for adding one or more L-nucleotides to the 3? end of a first L-nucleic acid, wherein the method comprises the step of reacting the one or more L-nucleotides with the first L-nucleic acid in the presence of a protein comprising an enzymatic activity exhibiting moiety, wherein the enzymatic activity is capable of adding one or more L-nucleotides to the 3? end of the first L-nucleic acid.
    Type: Grant
    Filed: December 5, 2017
    Date of Patent: May 25, 2021
    Assignee: APTARION biotech AG
    Inventors: Andreas Pech, Ralf David, Florian Jarosch, Michael Jahnz, Sven Klussmann
  • Patent number: 10982244
    Abstract: The present disclosure relates to modified homoserine dehydrogenase and a method for producing homoserine or a homoserine-derived L-amino acid using the same.
    Type: Grant
    Filed: April 10, 2019
    Date of Patent: April 20, 2021
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Su Yon Kwon, Kwang Woo Lee, Lan Huh, Kyungrim Kim, Mina Baek, Seung-ju Son, Jaemin Lee
  • Patent number: 10975400
    Abstract: A method for constructing an ALA production bacterial strain, the method enhances the activity of related enzymes promoting the synthesis of oxaloacetate and in the 5-aminolevulinic acid (ALA) production bacterial strain, or introducing exogenous related enzymes promoting the synthesis of oxaloacetate, such as phosphoenolpyruvate carboxylase or pyruvate carboxylase, and/or reducing the activity of related enzymes in the downstream metabolic pathway of succinyl coenzyme A in the bacterial strain, such as succinyl coenzyme A synthetase or succinate dehydrogenase, and/or reducing the activity of phosphoenolpyruvate carboxylated kinase and/or malic enzyme. An ALA high-yield bacterial strain constructed by utilizing the method, and method for utilizing the bacterial strain to prepare ALA.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: April 13, 2021
    Assignee: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
    Inventors: Ping Zheng, Jiuzhou Chen, Wei Pu, Jibin Sun, Xinyang Wu, Yanhe Ma
  • Patent number: 10975406
    Abstract: The invention provides compositions and methods for repeatable directed endonucleases (RDEs) and methods for repeatedly, and specifically cleaving DNA offset from the RDE's DNA recognition sequence on the target nucleic acid rather than within the DNA recognition sequence. Conservation of the recognition sequence of the target nucleic acid enables for re-localization of an RDE back to the DNA recognition sequence for further cleavage. The RDEs and methods of the invention are useful in applications including, but not limited to, recording data into a genome, timing the order of biochemical pathway events, efficient genome engineering and encoding lagged cellular death.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: April 13, 2021
    Assignee: Massachusetts Institute of Technology
    Inventors: Joseph M. Jacobson, Noah Michael Jakimo
  • Patent number: 10961554
    Abstract: The present disclosure relates to a novel promoter and a method for producing L-amino acids using the promoter, and more specifically, to a novel polynucleotide having promoter activity, a vector and a microorganism of the genus Corynebacterium comprising the polynucleotide, a method for producing L-amino acids and a fermented composition using the microorganism, and a fermented composition.
    Type: Grant
    Filed: March 27, 2019
    Date of Patent: March 30, 2021
    Assignee: CJ Cheiljedang Corporation
    Inventors: Ji Yeon Lee, Jin Sook Chang, Hyung Joon Kim, Byoung Hoon Yoon, Sun Hyoung Choi, Yunjung Choi
  • Patent number: 10920231
    Abstract: The present invention provides for a system for increasing the production of a compound using an artificial positive feedback loop (APFL). In some embodiments, the system diverts a compound produced in a first metabolic pathway into a second metabolic pathway in order to produce a compound of interest.
    Type: Grant
    Filed: January 27, 2015
    Date of Patent: February 16, 2021
    Assignee: The Regents of the University of California
    Inventor: Dominique Loque
  • Patent number: 10913959
    Abstract: A method for isomerizing a double bond is provided. A substrate is exposed to an isomerase enzyme, wherein the isomerase enzyme comprises a redox-regulated ligand switch and heme b cofactor. A reducing agent changes iron (III) to iron (II). The enzyme isomerizes double bonds in the iron (II) state but not in the iron (III) state. In one embodiment, the enzyme is homologous with 15-cis-?-carotene isomerase (Z-ISO).
    Type: Grant
    Filed: July 11, 2018
    Date of Patent: February 9, 2021
    Assignee: Research Foundation of the City University of New York
    Inventors: Eleanore T. Wurtzel, Jesús Beltrán
  • Patent number: 10913958
    Abstract: The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.
    Type: Grant
    Filed: January 10, 2018
    Date of Patent: February 9, 2021
    Assignee: LANZATECH NEW ZEALAND LIMITED
    Inventor: Michael Koepke
  • Patent number: 10906947
    Abstract: The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: February 2, 2021
    Assignee: Bolt Threads, Inc.
    Inventors: Joshua Kittleson, Thomas Stevens, Rena Hill, Carlos Gustavo Pesce
  • Patent number: 10889821
    Abstract: Presented herein are biocatalysts and methods for converting C1-containing materials to organic acids such as muconic acid or adipic acid.
    Type: Grant
    Filed: August 23, 2019
    Date of Patent: January 12, 2021
    Assignee: Alliance for Sustainable Energy, LLC
    Inventors: Calvin A. Henard, Michael T. Guarnieri
  • Patent number: 10889843
    Abstract: The present invention relates to an L-lysine-producing microorganism of the genus Corynebacterium and a method for producing L-lysine using the same.
    Type: Grant
    Filed: July 27, 2016
    Date of Patent: January 12, 2021
    Assignee: CJ Cheiljedang Corporation
    Inventors: Hyo Jeong Byun, Yoon Hee Chung, Hyung Joon Kim, Sun Young Lee, Hyun Koo Nam, Sun Mi Park, Sang Mok Lee
  • Patent number: 10870836
    Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.
    Type: Grant
    Filed: August 22, 2017
    Date of Patent: December 22, 2020
    Assignee: Illumina, Inc.
    Inventors: Cheng-Yao Chen, Erin Bomati, Molly He
  • Patent number: 10865404
    Abstract: The present invention provides an aspartase mutant, a recombinant expression vector and recombinant bacterium containing the aspartase mutant, and the use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the aspartase mutant is as set forth in SEQ ID NO: 1. In the aspartase mutant of the present invention, on the basis of wild type aspartase (with an amino acid sequence as set out in SEQ ID NO: 3), glutamic acid at position 427 is mutated into glutamine. In the present invention, by mutating the amino acid residue at position 427 into glutamine, the polar environment near an active site is changed, and thus ammonia supply during substrate reaction is further facilitated, thereby improving an enzyme activity, enhancing the ability of the enzyme in synthesizing a ?-amino acid, and providing a practical and effective strategy for industrial production of the ?-amino acid.
    Type: Grant
    Filed: June 15, 2020
    Date of Patent: December 15, 2020
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Zhiming Rao, Yaling Wang, Jiamin Chen, Taowei Yang, Meijuan Xu, Xian Zhang, Minglong Shao, Jianing Zhang, Anqi Peng, Shuping Xu, Meiqi Wu
  • Patent number: 10858635
    Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
    Type: Grant
    Filed: March 20, 2020
    Date of Patent: December 8, 2020
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Paulina Elena Salim, Derek J. Smith, Jacob Janey, Birgit Kosjek, Weng Lin Tang, Helen Hsieh, Son Q. Pham
  • Patent number: 10851393
    Abstract: The present disclosure describes a genetically engineered a KASIII-independent fatty acid biosynthetic pathway that makes use of the promiscuous nature of the rest of the FAS enzymes (3-ketoacyl-ACP synthetase, 3-ketoacyl-ACP reductase, 3-hydroxyacyl ACP dehydrase, enoyl-ACP reductase) to bypass the KASIII step by providing a Co-A precursor of two or higher than two carbons (such as the four carbon butyryl-CoA) as the starting molecule. Since many CoA-based starter molecules can be supplied for the fatty acid synthesis, much more diversified products can be obtained with various carbon-chain lengths. As such, this disclosure will serve as a powerful and efficient platform to produce low to medium chain length products carrying many different functional groups.
    Type: Grant
    Filed: February 22, 2016
    Date of Patent: December 1, 2020
    Assignee: William Marsh Rice University
    Inventors: Ka-Yiu San, Xian Zhang, Hui Wu, Dan Wang
  • Patent number: 10851362
    Abstract: Methods for producing intein-modified proteases are provided. Expression cassettes and vectors for using to genetically engineer hosts are described. Hosts genetically engineered to express one or more intein-modified proteases using expression cassettes and vectors of the invention are also provided. Methods to produce a protease and regulate its activity are described.
    Type: Grant
    Filed: July 5, 2018
    Date of Patent: December 1, 2020
    Assignee: AGRIVIDA, INC.
    Inventors: R. Michael Raab, Binzhang Shen, Gabor Lazar, Humberto De La Vega
  • Patent number: 10837007
    Abstract: The invention relates to recombinant fusion proteins comprising a fibrinogenolytic enzyme having an amino acid sequence that is C-terminally and/or N-terminally linked to the amino acid sequence of at least one high-molecular inert stabilization domain with a molecular weight of >50 kDa, for the prevention or treatment of adhesions at tissues or organs, in particular peritoneal adhesions following surgical interventions.
    Type: Grant
    Filed: August 20, 2015
    Date of Patent: November 17, 2020
    Assignee: Akesion GmbH
    Inventors: Klaus Rübsamen, Stephan Witte
  • Patent number: 10837006
    Abstract: The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: November 17, 2020
    Assignee: Novozymes A/S
    Inventors: Astrid Benie, Morten Gjermansen, Peter Rahbek Oestergaard
  • Patent number: 10829791
    Abstract: The present invention relates to a method of producing itaconic acid. Further the present invention relates to nucleic acids encoding an aconitate-delta-isomerase (ADI) and trans-aconitate decarboxylase (TAD) and uses of such nucleic acids. Provided is additionally a recombinant host cell engineered to overexpress nucleic acids of the present invention. Furthermore an expression cassette and a vector are provided which include the respective nucleic acid.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: November 10, 2020
    Assignee: RHEINISCH-WESTFAELISCHE TECHNISCHE HOCHSCHULE (RWTH) AACHEN
    Inventors: Michael Boelker, Wolfgang Buckel, Sandra Hartmann, Lars M. Blank, Nick Wierckx, Elena Geiser
  • Patent number: 10822597
    Abstract: The invention relates to improved alkaline phosphatases, pharmaceutical compositions comprising improved alkaline phosphatases and the use of improved alkaline phosphatases for preventing, treating or curing diseases.
    Type: Grant
    Filed: February 7, 2018
    Date of Patent: November 3, 2020
    Assignee: AM-Pharma B.V.
    Inventors: Willem Raaben, Luigi Johannes Cornelius Jonk, Erik Jan Van Den Berg, Andrea Van Elsas, José Luis Millán