Abstract: The present invention relates to an L-lysine-producing microorganism of the genus Corynebacterium and a method for producing L-lysine using the same.
Type:
Grant
Filed:
July 27, 2016
Date of Patent:
January 12, 2021
Assignee:
CJ Cheiljedang Corporation
Inventors:
Hyo Jeong Byun, Yoon Hee Chung, Hyung Joon Kim, Sun Young Lee, Hyun Koo Nam, Sun Mi Park, Sang Mok Lee
Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.
Abstract: The present invention provides an aspartase mutant, a recombinant expression vector and recombinant bacterium containing the aspartase mutant, and the use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the aspartase mutant is as set forth in SEQ ID NO: 1. In the aspartase mutant of the present invention, on the basis of wild type aspartase (with an amino acid sequence as set out in SEQ ID NO: 3), glutamic acid at position 427 is mutated into glutamine. In the present invention, by mutating the amino acid residue at position 427 into glutamine, the polar environment near an active site is changed, and thus ammonia supply during substrate reaction is further facilitated, thereby improving an enzyme activity, enhancing the ability of the enzyme in synthesizing a ?-amino acid, and providing a practical and effective strategy for industrial production of the ?-amino acid.
Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
Type:
Grant
Filed:
March 20, 2020
Date of Patent:
December 8, 2020
Assignee:
Codexis, Inc.
Inventors:
Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Paulina Elena Salim, Derek J. Smith, Jacob Janey, Birgit Kosjek, Weng Lin Tang, Helen Hsieh, Son Q. Pham
Abstract: Methods for producing intein-modified proteases are provided. Expression cassettes and vectors for using to genetically engineer hosts are described. Hosts genetically engineered to express one or more intein-modified proteases using expression cassettes and vectors of the invention are also provided. Methods to produce a protease and regulate its activity are described.
Type:
Grant
Filed:
July 5, 2018
Date of Patent:
December 1, 2020
Assignee:
AGRIVIDA, INC.
Inventors:
R. Michael Raab, Binzhang Shen, Gabor Lazar, Humberto De La Vega
Abstract: The present disclosure describes a genetically engineered a KASIII-independent fatty acid biosynthetic pathway that makes use of the promiscuous nature of the rest of the FAS enzymes (3-ketoacyl-ACP synthetase, 3-ketoacyl-ACP reductase, 3-hydroxyacyl ACP dehydrase, enoyl-ACP reductase) to bypass the KASIII step by providing a Co-A precursor of two or higher than two carbons (such as the four carbon butyryl-CoA) as the starting molecule. Since many CoA-based starter molecules can be supplied for the fatty acid synthesis, much more diversified products can be obtained with various carbon-chain lengths. As such, this disclosure will serve as a powerful and efficient platform to produce low to medium chain length products carrying many different functional groups.
Type:
Grant
Filed:
February 22, 2016
Date of Patent:
December 1, 2020
Assignee:
William Marsh Rice University
Inventors:
Ka-Yiu San, Xian Zhang, Hui Wu, Dan Wang
Abstract: The invention relates to recombinant fusion proteins comprising a fibrinogenolytic enzyme having an amino acid sequence that is C-terminally and/or N-terminally linked to the amino acid sequence of at least one high-molecular inert stabilization domain with a molecular weight of >50 kDa, for the prevention or treatment of adhesions at tissues or organs, in particular peritoneal adhesions following surgical interventions.
Abstract: The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Grant
Filed:
January 20, 2017
Date of Patent:
November 17, 2020
Assignee:
Novozymes A/S
Inventors:
Astrid Benie, Morten Gjermansen, Peter Rahbek Oestergaard
Abstract: The present invention relates to a method of producing itaconic acid. Further the present invention relates to nucleic acids encoding an aconitate-delta-isomerase (ADI) and trans-aconitate decarboxylase (TAD) and uses of such nucleic acids. Provided is additionally a recombinant host cell engineered to overexpress nucleic acids of the present invention. Furthermore an expression cassette and a vector are provided which include the respective nucleic acid.
Abstract: The invention relates to improved alkaline phosphatases, pharmaceutical compositions comprising improved alkaline phosphatases and the use of improved alkaline phosphatases for preventing, treating or curing diseases.
Type:
Grant
Filed:
February 7, 2018
Date of Patent:
November 3, 2020
Assignee:
AM-Pharma B.V.
Inventors:
Willem Raaben, Luigi Johannes Cornelius Jonk, Erik Jan Van Den Berg, Andrea Van Elsas, José Luis Millán
Abstract: The present invention provides a ?-agarase, a composition containing the same and applications thereof. The present ?-agarase provides the field a novel alternative and is favorable for the industrial utilities of the hydrolysis products of agarose. Furthermore, the present agarase is particularly modified for heterologous production by prokaryotic expression systems, and thereby is favorable for commercial use.
Type:
Grant
Filed:
March 20, 2017
Date of Patent:
November 3, 2020
Assignee:
AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE
Abstract: Disclosed is a microorganism of Escherichia sp. producing O-acetyl homoserine, and a method of producing O-acetyl homoserine in high yield using the microorganism.
Type:
Grant
Filed:
September 20, 2019
Date of Patent:
October 27, 2020
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Hyun Ah Kim, Ju Hee Seo, Yong Uk Shin, So Young Kim, Sang Kyoum Kim, Kwang Ho Na, Jee Yeon Bae, Sung Kwang Son, Hye Ryun Yoo, Jin Geun Choi
Abstract: The present application relates to a microorganism of the genus Escherichia producing L-tryptophan and, more specifically, to a microorganism of the genus Escherichia with improved activity of producing L-tryptophan by weakening or inactivating the activity of endogenous 6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase. Additionally, the present application relates to a method for producing L-tryptophan using the microorganism of the genus Escherichia.
Type:
Grant
Filed:
June 23, 2015
Date of Patent:
October 27, 2020
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Ki Yong Cheong, Kyung Rim Kim, Seok Myung Lee, Kwang Ho Lee, Keun Cheol Lee, Hyeong Pyo Hong
Abstract: Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them.
Type:
Grant
Filed:
July 31, 2017
Date of Patent:
October 13, 2020
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Sung Jae Yang, Young Mi Lee, Il Hyang Park, Chan-Hyoung Lee, Hyun Kug Cho, Seong Bo Kim, Yang Hee Kim, Seung Won Park
Abstract: A process for preparing a milk protein hydrolysate comprising hydrolysing a milk-based proteinaceous material with a microbial alkaline serine protease in combination with bromelain, a protease from Aspergillus and a protease from Bacillus.
Type:
Grant
Filed:
March 21, 2016
Date of Patent:
October 13, 2020
Assignee:
Societe des Produits Nestle S.A.
Inventors:
Francoise Maynard, Delphine Salvatore, Anne Thevenier
Abstract: Provided is a method of producing L-amino acids by using a recombinant coryneform microorganism in which the expression of a target gene is weakened by using a gene transcription inhibition method.
Type:
Grant
Filed:
March 20, 2017
Date of Patent:
October 13, 2020
Assignee:
CJ CHEILJEDANG CORP.
Inventors:
Jun Ok Moon, Sang Jo Lim, Do Hyun Kwon, Kwang Ho Lee, Hyun Won Bae
Abstract: Provided is a transformed cell improved in C4 dicarboxylic acid productivity. A transformed cell containing a foreign polynucleotide encoding a polypeptide consisting of an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence represented by SEQ ID NO: 22 or an amino acid sequence having an identity of at least 80% with any of the sequences.
Abstract: Photosynthetic microorganisms with broadened light absorption capability and increased photosynthetic activity are described. Broadened light absorption is achieved by modifying the microorganism to utilize non-native bilins. Increased photosynthetic activity results from the broadened light absorption and can also result from a decrease in self-shading. The microorganisms include Cyanobacteria, including modified Cyanobacteria.
Type:
Grant
Filed:
July 7, 2016
Date of Patent:
September 29, 2020
Assignees:
LUMEN BIOSCIENCE, INC., RELIANCE HOLDING USA, INC.
Abstract: Isolated polynucleotides encoding a BREX system are provided. Accordingly there is provided an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ and at least one of pglX, pglXI, brxP, brxHI, brxHII, brxL, brxD, brxA, brxB, brxF, and brxE, with the proviso that said BREX system does not comprise pglW, and wherein said BREX system confers phage resistance to a bacteria recombinantly expressing same; Also provided is an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ, pglX, pglW and at least one of brxD and brxHI, and wherein said BREX system confers phage resistance to a bacteria recombinantly expressing same. Also provided are compositions and methods for conferring phage resistance to bacteria or for conferring bacterial susceptibility to phages.