Abstract: The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.
Type:
Grant
Filed:
December 17, 2010
Date of Patent:
November 27, 2018
Assignee:
DANISCO US INC.
Inventors:
Christina Gutierrez, Colin Mitchinson, Tom T. Huang, Bruce A. Diner, Paul Joseph Fagan, William D. Hitz
Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.
Type:
Grant
Filed:
June 14, 2016
Date of Patent:
September 25, 2018
Assignee:
Genomatica, Inc.
Inventors:
Mark J. Burk, Priti Pharkya, Stephen J. Van Dien, Anthony P. Burgard, Christophe H. Schilling
Abstract: Methods for producing intein-modified proteases are provided. Expression cassettes and vectors for using to genetically engineer hosts are described. Hosts genetically engineered to express one or more intein-modified proteases using expression cassettes and vectors of the invention are also provided. Methods to produce a protease and regulate its activity are described.
Type:
Grant
Filed:
October 3, 2013
Date of Patent:
August 14, 2018
Assignee:
AGRIVIDA, INC.
Inventors:
R. Michael Raab, Binzhang Shen, Gabor Lazar, Humberto De La Vega