Patents Examined by Trevor L Kane
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Patent number: 11788105Abstract: Described are methods of using a lipase for hydrolysis and esterification. In a first method of producing a medium chain fatty acid by hydrolysis, the method comprises providing a polypeptide with at least 90% degree of identity to SEQ ID No. 3, and contacting the polypeptide with a medium chain fatty acid ester and water to produce the medium chain fatty acid. In a second method of forming an ester, the method comprises providing a polypeptide with at least 90% degree of identity to SEQ ID No. 3; and contacting the polypeptide with a long chain fatty acid, an alcohol, and water to form the ester of the long chain fatty acid and the alcohol.Type: GrantFiled: December 30, 2020Date of Patent: October 17, 2023Assignee: Wilmar International LimitedInventors: Ren Liang Yang, Hong Fang Zhang, Weijian Ye, Mong Jie Andre Ng, Kien Truc Giang Nguyen
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Patent number: 11781102Abstract: We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.Type: GrantFiled: April 25, 2017Date of Patent: October 10, 2023Assignee: Trizell Ltd.Inventors: Hanna P. Lesch, Joonas Malinen, Eevi Lipponen, Anniina Valkama, Hanna Leinonen
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Patent number: 11746067Abstract: The present invention comprises methods and compositions comprising a microalgae consortium. A composition comprises at least one genera of microalgae flocculated by Paenibacillus polymyxa strain, Strain 2, deposited under the Budapest Treaty as ATCC Accession No. PTA-12841. Methods of treating the soil comprise adding a composition of the present invention to soil.Type: GrantFiled: October 29, 2019Date of Patent: September 5, 2023Assignee: Core Intellectual Properties Holdings, LLCInventors: Luke Blotsky, Karin L. Hastings, Derex Q. Zellars
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Patent number: 11732277Abstract: A method for producing high purity iron oxide nanoparticles using nanoparticle-producing cells, including: a) a pre-growth step that includes amplifying the nanoparticle-producing cell(s) in a pre-growth and/or fed-batch medium/media, and b) a growth step that includes amplifying the nanoparticle-producing cell(s) originating from the pre-growth step in a growth and/or fed-batch medium/media, wherein the pre-growth and/or growth and/or fed-batch medium/media comprise(s), per kilogram or liter of pre-growth and/or growth and/or fed-batch medium/media: i) no more than 0.005 gram of yeast extract, and ii) no more than 0.001 gram of CMR agent selected from boric acid and nitrilotriacetic acid, wherein the fed-batch medium when it is present is a medium that supplements the pre-growth and/or growth medium/media, and wherein more nanoparticles are produced in the growth step than in the pre-growth step.Type: GrantFiled: October 1, 2019Date of Patent: August 22, 2023Assignee: NANOBACTERTEInventor: Edouard Alphandery
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Patent number: 11718842Abstract: A dehydrated composition containing a particle encapsulating one or more microorganisms is provided. The composition is useful for controlling the release of the microorganisms following rehydration and propagation within the particle.Type: GrantFiled: July 24, 2019Date of Patent: August 8, 2023Assignee: LAVIE BIO LTD.Inventors: Michael Ionescu, Galit Kuznets, Amir Bercovitz, Yossi Singer, Hagai Karchi
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Patent number: 11718868Abstract: Lipase activity can be detected with a biosensor that includes a quantum dot adhered to a construct having a lipase-cleavable ester to attach a fluorophore acceptor configured as a Förster resonance energy transfer (FRET) acceptor to the QD when the construct is bound thereto. Cleavage of the ester by a lipase results in a measurable reduction in FRET. In further embodiments, the cleavable ester can be used to detect esterase activity, or the ester could be replaced with a glycosidic linkage to detect glycoside activity.Type: GrantFiled: January 29, 2021Date of Patent: August 8, 2023Assignee: The Government of the United States of America, as represented by the Secretary of the NavyInventors: Igor Medintz, Joyce A. Breger, Kimihiro Susumu, Sebastian Diaz, Jesper Brask
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Patent number: 11667899Abstract: The present invention provides compositions, methods, and kits related to reverse transcriptases derived from E.r. maturase.Type: GrantFiled: June 27, 2018Date of Patent: June 6, 2023Assignee: YALE UNIVERSITYInventors: Anna Marie Pyle, Chen Zhao
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Patent number: 11613728Abstract: An object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in which at least one of the amino acid residues at positions 6, 65, 230, 231, and 275 in the amino acid sequence represented by SEQ ID NO: 2 has been substituted with another amino acid residue (mutated OrfB), and is capable of producing eicosapentaenoic acid (EPA), and the like.Type: GrantFiled: August 9, 2019Date of Patent: March 28, 2023Assignee: KYOWA HAKKO BIO CO., LTD.Inventors: Tohru Dairi, Yasuharu Satoh, Shohei Hayashi, Mai Naka, Tetsuro Ujihara
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Patent number: 11591563Abstract: A liquid sporulation method is described. A liquid culture is prepared by adding bacterial cells to a sporulation broth. The liquid culture is incubated. Spores are harvested from the incubated liquid culture by separating the spores from a culture supernatant. In some embodiments, the harvested spores are suspended in an aqueous medium to form a spore suspension. At least a portion of the culture supernatant is combined with the spore suspension.Type: GrantFiled: July 24, 2020Date of Patent: February 28, 2023Assignee: American Sterilizer CompanyInventor: Stephen Norris
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Patent number: 11566251Abstract: The present disclosure relates to the fields of biotechnology, molecular biology and genetic engineering. In particular, the present disclosure relates to a genetically modified alga comprising a recombinant cytochrome c6 gene, methods of producing the same and applications thereof. The present disclosure also relates to a codon optimised nucleic acid sequence encoding a cytochrome c6 polypeptide, expression cassette, vectors and host cell thereof. In an embodiment, the present disclosure also relates to a method of increasing biomass and photosynthetic efficiency of algae.Type: GrantFiled: December 2, 2019Date of Patent: January 31, 2023Assignee: Reliance Industries LimitedInventors: Arun Banerjee, Gautam Das, Sonal Santosh Patil, Sourav Sanyal, Smita Dattatraya Patil, Santanu Dasgupta
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Patent number: 11535543Abstract: A medium material for removing phenol contamination from groundwater, a method of producing the same, and use of the same id disclosed. In at least some embodiments, the medium material is a granular material which has an average particle diameter of 0.5-1.5 cm and is formed from a bacteria-entrapping solution, a manganese sand filter material, modified bentonite, and biochar at a mass ratio of 1:0.2-0.4:0.2-0.4:0.1-0.2 by a series of processes including strain culturing, catalysis, mixing, solidification, and the like. The medium material can remove phenol from groundwater, is a safe and environment-friendly material, has a long service life, and/or achieves waste treatment with waste.Type: GrantFiled: March 19, 2020Date of Patent: December 27, 2022Assignee: Chinese Research Academy of Environmental SciencesInventors: Yonghai Jiang, Xinying Lian, Beidou Xi, Yu Yang, Yongfeng Jia, Fu Xia, Xu Han
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Patent number: 11491194Abstract: Compositions and methods for mucosal delivery of agents are provided. The emulsion compositions are intended for administration to a mucosal surface, such as oral, gastrointestinal and nasal mucosa. The emulsion compositions provided contain one or more mucoadhesive proteins and an agent to be delivered. Methods for delivery of agents using the compositions provided herein are also provided.Type: GrantFiled: September 12, 2019Date of Patent: November 8, 2022Assignee: Virun, Inc.Inventor: Philip J. Bromley
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Patent number: 11466244Abstract: A composition includes at least one carrier containing a polysaccharide, at least one antioxidant and at least one amino acid selected from cysteine, lysine, alanine and arginine. Such a composition can be used for protection of microorganisms during drying, storage and/or reconstitution. A culture powder, a process of making the culture powder, and products containing the culture powder are also disclosed.Type: GrantFiled: June 10, 2020Date of Patent: October 11, 2022Assignee: Societe des Produits Nestle S.A.Inventors: Edwin Ananta, Biljana Bogicevic, Carsten Cramer, Alexandra Dubois, Judith Gaulocher, Katja Johnson, Jeroen Andre Muller, Matthias Perren, Guenolee Eliane Marie Prioult, Sabine Sres, Wilbert Sybesma
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Patent number: 11459593Abstract: The present invention provides a Dendrobium officinale (D. officinale) endophytic fungus strain and an extracellular polysaccharide produced thereby as well as an extraction method and application of the extracellular polysaccharide. The extraction process of the extracellular polysaccharide includes: activating a strain; culturing seed liquid; fermenting and culturing; filtering the fermented liquid under vacuum; concentrated the filtrate under vacuum; conducting alcohol precipitation on the concentrated liquid; precipitating and centrifuging; dialyzing after dissolving the precipitates in water; freezing and drying the dialysate under vacuum; and obtaining the extracellular polysaccharide. The D. officinale endophytic fungus strain belongs to Fusarium solani, and the extracellular polysaccharide produced thereby has inhibitory effects on Escherichia coli, Staphylococcus aureus, Salmonella ssp., and Bacillus subtilis.Type: GrantFiled: December 13, 2017Date of Patent: October 4, 2022Assignee: SOUTH CHINA UNIVERSITY OF TECHNOLOGYInventors: Wenyong Lou, Yingjie Zeng, Hongfeng Wang, Minhua Zong, Jing Liang
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Patent number: 11419905Abstract: The disclosure discloses Bacteroides ovatus for relieving endotoxin infection and application thereof, and belongs to the technical field of microorganisms. The Bacteroides ovatus CCFM1021 of the disclosure has low immunogenicity, and can reduce the content of pro-inflammatory factors and increase the concentration of anti-inflammatory factors in the blood of mice infected with endotoxin, up-regulate the number of Foxp3+ regulatory T cells and stabilize the composition of the intestinal microbiota. A pharmaceutical composition of the Bacteroides ovatus CCFM1021 for relieving endotoxin infection has broad application prospects.Type: GrantFiled: October 28, 2020Date of Patent: August 23, 2022Assignee: Jiangnan UniversityInventors: Wei Chen, Qixiao Zhai, Huizi Tan, Wenwei Lu, Fengwei Tian, Jianxin Zhao, Hao Zhang
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Patent number: 11377670Abstract: The present disclosure discloses a method for preparing vanillin by fermentation with eugenol as a substrate, preparing vanillin by a mixed fermentation of Penicillium simplicissimum OMK-68 and Bacillus sp. OMK-69, its potency reached 35 g/L, the mass conversion rate of eugenol reaches 83.3%. Penicillium simplicissimum OMK-68 in the present disclosure can use eugenol as a substrate for fermentation to prepare coniferyl alcohol, and its fermentation effect and substrate utilization rate are far better than wild-type Penicillium simplicissimum. The Bacillus sp. OMK-69 in the present disclosure can use coniferyl alcohol as a substrate for fermentation to prepare vanillin, and its fermentation effect and substrate utilization rate are far better than wild-type Bacillus sp.Type: GrantFiled: December 30, 2020Date of Patent: July 5, 2022Assignee: Xiamen Oamic Biotechnology Co., Ltd.Inventors: Chenguang Xing, Xijing Zhao, Yarou Zeng, Huanyan Lan, Wei Liu, Gang Liu, Zhimin Deng