Abstract: A new and distinct variety of peach tree, denominated ‘UF ONE’, has a low winter chilling requirement estimated at 150 chill units (cu). The tree is of medium size, has a moderate vigorous and semi-spreading growth habit. It has showy, pink flowers, and leaves with globose glands. Trees of ‘UF ONE’ are self-fertile and regularly bear heavy annual crops of early season fruit that are large for its ripening season. Fruit are uniformly firm and yellow with non-melting, clingstone flesh. Fruit are slightly oval, and uniform with substantially symmetrical shape, and have an attractive 40% bright red skin. The fruit of ‘UF ONE’ blooms about 3 days before and ripens about 8 days after ‘UFBeauty’ peach after the first week in May at Gainesville, Fla.