Patents Examined by William H. Beismer
  • Patent number: 6054287
    Abstract: A mathematical model, the membranes and devices based upon that model to optimize protocols for the addition or removal of cryoprotectant to or from biological cells, and a method to observe the biological cells and obtain the data to implement the models. This disclosure describes the use of four equations to predict optimal protocols to add or remove cryoprotectant to or from biological cells. The equations particularly require experimentally found data, specific to cell-type and species, regarding the osmotic tolerance of the cells, where osmotic tolerance refers to the cells ability to shrink or swell to various changes in osmolality without injury. The equations further require the cryoprotectant permeability coefficient and the water permeability coefficient of the particular cells' plasma membrane.
    Type: Grant
    Filed: February 27, 1998
    Date of Patent: April 25, 2000
    Assignee: Methodist Hospital of Indiana, Inc.
    Inventors: Dayong Gao, John K. Critser
  • Patent number: 5536662
    Abstract: This invention encompasses porous microchambers which contain cells and permit liquid to flow in and out of the chamber while retaining cells within the chamber. These porous microchambers serve as disposable devices for placing cells in a microflowchamber so that properties of the cells within the porous microchamber can be measured.
    Type: Grant
    Filed: December 2, 1993
    Date of Patent: July 16, 1996
    Assignee: Molecular Devices Corporation
    Inventors: Gillian M. K. Humphries, Donald L. Miller, Jeffrey M. Libby, Henry L. Schwartz