Patents Examined by William. M. Moore
  • Process for preparing doxorubicin
    Patent number: 6210930
    Abstract: The present invention is directed to a process for improving daunorubicin and doxorubicin production by means of a recombinant microorganism in which a gene of daunorubicin metabolism is mutated. The mutated gene is preferably dnrU and/or dnrX.
    Type: Grant
    Filed: January 14, 1999
    Date of Patent: April 3, 2001
    Assignee: Pharmacia & Upjohn, S.P.A.
    Inventors: Silvia Filippini, Natalia Lomovskaya, Leonid Fonstein, Anna Luisa Colombo, C. Richard Hutchinson, Sharee L. Otten, Umberto Breme
  • Modified prokaryotic glucose isomerase enzymes with altered pH activity profiles
    Patent number: 5340738
    Abstract: A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pK.sub.a of the metal coordinating ligands or altering the K.sub.ass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.
    Type: Grant
    Filed: January 4, 1991
    Date of Patent: August 23, 1994
    Assignees: Gist-brocades, N.V., Plant Genetic Systems, N.V.
    Inventors: Anne-Marie Lambeir, Ignace Lasters, Nadir Mrabet, Wilhelmus J. Quax, Jan M. Van der Laan, Onno Misset