Patents Examined by William W. Moore
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Patent number: 9243301Abstract: A single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment can cleave a protein of the exocytic fusion apparatus of a nociceptive sensory afferent; a Targeting Moiety that can bind to a Binding Site on the nociceptive sensory afferent, which Binding Site can undergo endocytosis to be incorporated into an endosome within the nociceptive sensory afferent; a protease cleavage site at which site the fusion protein is cleavable by a protease, which is located between the non-cytotoxic protease and the Targeting Moiety; and a translocation domain that can translocate the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the nociceptive sensory afferent; wherein the Targeting Moiety is BAM, ?-endorphin, bradykinin, substance P, dynorphin and/or nociceptin. Nucleic acid sequences encoding the fusion proteins, methods of preparing same and uses thereof are also described.Type: GrantFiled: March 13, 2012Date of Patent: January 26, 2016Assignees: Allergan, Inc., Ipsen Bioinnovation LimitedInventors: Keith Foster, John Chaddock, Philip Marks, Patrick Stancombe, Kei Roger Aoki, Joseph Francis, Lance Steward
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Patent number: 9243042Abstract: It is an object to provide a protein having a dockerin, which is suited to production in yeasts and other eukaryotic microorganism in which sugar chain modification is predicted, and which provides excellent cohesin-dockerin binding ability, along with a use thereof. The present invention uses, as a protein for constructing a protein complex using a scaffolding protein having a type I cohesin from Clostridium thermocellum, a protein having a dockerin having at least one dockerin-specific sequence which is a dockerin-specific sequence associated with cohesin binding in type I dockerins from C. thermocellum, and which either has no intrinsic predicted N-type sugar chain modification site or has aspartic acid substituted for the asparagine of an intrinsic predicted N-type sugar chain modification site.Type: GrantFiled: March 25, 2011Date of Patent: January 26, 2016Assignee: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHOInventors: Hiroaki Suzuki, Takao Imaeda, Katsunori Kohda
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Patent number: 9187738Abstract: The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position and at a location in an external loop distal to the active site of the enzyme which is more hydrophilic; or c) a substitution or insertion at one or more of positions disclosed herein or a deletion at one or more positions disclosed herein. The invention also relates to polypeptide produced by the method and to novel nucleic acids.Type: GrantFiled: December 19, 2011Date of Patent: November 17, 2015Assignee: DUPONT NUTRITION BIOSCIENCES APSInventors: Andrei Miasnikov, Richard R. Bott, Jens Frisbæk Sørensen
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Patent number: 9181574Abstract: Methods are provided measuring L-lysine using a variant enzyme, an L-lysine measurement kit, and an enzyme sensor. Variant L-amino acid oxidase having a predetermined amino acid mutation, and having oxidase activity that is highly substrate-specific for L-lysine; a method for measuring L-lysine using this variant enzyme; an L-lysine measurement kit; and an enzyme sensor are also provided.Type: GrantFiled: July 29, 2014Date of Patent: November 10, 2015Assignees: AJINOMOTO CO., INC., TOYAMA PREFECTUREInventors: Yasuhisa Asano, Daisuke Matsui
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Patent number: 9175270Abstract: The invention relates to a method of modifying a yeast cell for the production of ethanol. According to some embodiments of the invention, the activity of the Gpd1 protein and/or the Gpd2 protein is reduced.Type: GrantFiled: October 29, 2008Date of Patent: November 3, 2015Assignee: Danisco US Inc.Inventors: Elke Nevoigt, Stephane Guillouet, Carine Bideaux, Sandrine Alfenore, Georg Hubmann
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Patent number: 9175297Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.Type: GrantFiled: April 17, 2012Date of Patent: November 3, 2015Assignee: Genomatica, Inc.Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun
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Patent number: 9169475Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.Type: GrantFiled: August 29, 2013Date of Patent: October 27, 2015Assignee: Novozymes A/SInventors: Allan Svendsen, Henriette Draborg
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Patent number: 9170262Abstract: Methods of qualitatively and/or quantitatively detecting the presence of mutations, modifications or impurities in a protein sample are provided. The methods utilize isotopically labeled variants of amino acids incorporated into proteins prior to protein digest to enable comparisons of two protein samples in bottom-up liquid chromatography.Type: GrantFiled: June 16, 2011Date of Patent: October 27, 2015Assignee: AbbVie, Inc.Inventors: Anton V. Manuilov, David H. Lee
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Patent number: 9131711Abstract: The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.Type: GrantFiled: May 23, 2014Date of Patent: September 15, 2015Assignee: Novozymes A/SInventors: Leonardo De Maria, Carsten Andersen, Lars Lehmann Hyllling Christensen, Soren Flensted Lassen, Peter Rahbek Ostergaard
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Patent number: 9133423Abstract: The present invention relates to novel subtilase variants exhibiting improvements relative to the parent subtilase in one or more properties including: wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g., cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.Type: GrantFiled: January 15, 2010Date of Patent: September 15, 2015Assignee: NOVOZYMES A/SInventors: Henriette Draborg, Vibeke Skovgaard Nielsen, Stefan Minning
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Patent number: 9127249Abstract: Provided is a novel method for preparing metabolites of atorvastatin using bacterial cytochrome P450, and a composition therefor, and more particularly, a composition for preparing 2-hydroxylated product of 4-hydroxylated product from atorvastatin including bacterial cytochrome P 450 BM3 (CYP102A1), CYP102A1 mutants, and chimeras derived from the CYP102A1 mutants, a kit therefor, and a method for preparing thereof.Type: GrantFiled: September 27, 2012Date of Patent: September 8, 2015Assignee: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITYInventors: Chul-Ho Yun, Dong-Hyun Kim, Ji-Yeon Kang, Sun-Ha Park, Sang-Hoon Ryu
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Patent number: 9090919Abstract: The present invention relates to a novel method of producing 3-hydroxypropionic acid from glycerol, and more particularly to a method of producing 3-hydroxypropionic acid by culturing in a glycerol-containing medium a mutant microorganism obtained by amplifying an aldehyde dehydrogenase-encoding gene in a microorganism having the abilities to produce coenzyme B12 and produce 3-hydroxypropionic acid using glycerol as a carbon source. The present invention enables the fermentation of glycerol even under microaerobic or aerobic conditions without having to add coenzyme B12. Thus, the invention will be very suitable for the development of biological processes for producing large amounts of 3-hydroxypropionic acid.Type: GrantFiled: October 29, 2009Date of Patent: July 28, 2015Assignee: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Chul-Ho Kim, Jeong-Woo Seo, Lianhua Luo, Baek Rock Oh, Pil-Soo Seo, Sun-Yeon Heo
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Patent number: 9080163Abstract: The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.Type: GrantFiled: May 9, 2014Date of Patent: July 14, 2015Assignee: Codexis, Inc.Inventors: Vesna Mitchell, Grzegorz Wojciechowski, Oscar Alvizo
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Patent number: 9080187Abstract: Described herein are methods, compositions and synthetic biology approaches for solvent production, including but not limited to butanol production. Described herein are recombinant bacteria and yeast strains which may be used in production of a solvent, including but not limited to butanol, from lignocellulosic and other plant-based feedstocks. Described herein are methods of producing solvents, including but not limited to butanol, using bacteria and yeast strains. Described herein are methods of producing organisms that display highly efficient butanol production.Type: GrantFiled: May 19, 2008Date of Patent: July 14, 2015Assignees: The Board of Trustees of the University of Illinois, Eastman Renewable Materials, LLCInventors: Hans P. Blaschek, Steven F. Stoddard, Zhen Shi
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Patent number: 9072736Abstract: Use of a therapeutic molecule, for the treatment of specific pain conditions, wherein the therapeutic molecule is a single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment can cleave a protein of the exocytic fusion apparatus of a nociceptive sensory afferent; a Targeting Moiety that can bind to a Binding Site on the nociceptive sensory afferent, which Binding Site can undergo endocytosis to be incorporated into an endosome within the nociceptive sensory afferent; a protease cleavage site at which site the fusion protein is cleavable by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety; and a translation domain that can translocate the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the nociceptive sensory afferent.Type: GrantFiled: January 27, 2012Date of Patent: July 7, 2015Assignees: Allergan, Inc., Syntaxin LimitedInventors: Keith Foster, John Chaddock, Philip Marks, Patrick Stancombe, K. Roger Aoki, Joseph Francis, Lance Steward
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Patent number: 9068013Abstract: In one aspect, the disclosure provides cross-linked materials that include multivalent lectins with at least two binding sites for glucose, wherein the lectins include at least one affinity ligand which is capable of competing with glucose for binding with at least one of said binding sites and is covalently linked to a cysteine residue of the lectins; and conjugates that include two or more separate affinity ligands bound to a conjugate framework, wherein the two or more affinity ligands compete with glucose for binding with the lectins at said binding sites and wherein conjugates are cross-linked within the material as a result of non-covalent interactions between lectins and affinity ligands on different conjugates. These materials are designed to release amounts of conjugate in response to desired concentrations of glucose. Depending on the end application, in various embodiments, the conjugates may also include a drug and/or a detectable label.Type: GrantFiled: July 22, 2011Date of Patent: June 30, 2015Assignee: SMART CELLS, INC.Inventors: Thomas M. Lancaster, Robert Butters, Naomi Choodnovskiy, Todd C. Zion
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Patent number: 9062291Abstract: A transglutaminase protein which is mutated to have improved heat resistance and/or pH stability. A mutation is introduced into WT transglutaminase at a cysteine residue capable of forming a disulfide bond (SS bond).Type: GrantFiled: August 14, 2009Date of Patent: June 23, 2015Assignee: AJINOMOTO CO., INC.Inventors: Keiichi Yokoyama, Mototaka Suzuki, Tatsuki Kashiwagi, Eiichiro Suzuki, Masayo Date, Seiichi Taguchi
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Patent number: 9046525Abstract: A method of counting protein subunits to determine the oligomeric state of an oligomeric protein complex includes tagging and expressing the protein subunits with a mass/charge tag and selectively removing each mass/charge tag. The number of protein subunits of the oligomeric complex corresponds to the number of mass/charge tags removed.Type: GrantFiled: August 1, 2011Date of Patent: June 2, 2015Assignee: California Institute of TechnologyInventors: Troy Walton, Chris Gandhi
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Patent number: 9005946Abstract: The invention provides variants of the Clostridium thermocellum endoglucanase (CelG) that have improved endoglucanase activity compared to the wild type enzyme. Also provided are related polynucleotides, compositions, vectors, host cells, and methods of use.Type: GrantFiled: March 10, 2010Date of Patent: April 14, 2015Assignee: Codexis, Inc.Inventors: Jie Yang, Xiyun Zhang, Ish Kumar Dhawan, Andrew Shaw
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Patent number: 9005950Abstract: The present invention relates to pectate lyase variants exhibiting alterations relative to a parent enzyme exhibiting pectate lyase activity as its major enzymatic activity; to a method of producing such enzymes; and to methods for using such enzymes in the textile, detergent and cellulose fiber processing industries. Compared to the parent enzyme, the pectate lyase variants of the present invention exhibit improved stability in detergents.Type: GrantFiled: September 13, 2013Date of Patent: April 14, 2015Assignee: Novozymes A/SInventors: Sanne Schoeder Glad, Carsten Andersen, Torben Vedel Borchert, Katja Salomon Johansen, Henrik Frisner, Mads Eskelund Bjornvad, Thomas Thisted