Patents Examined by William W. Moore
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Patent number: 8013137Abstract: Disclosed are novel enteropeptidase polypeptides, polynucleotides encoding the polypeptides, nucleotide constructs, vectors, host cells comprising the polynucleotides, and methods for producing the polypeptides and polynucleotides. Such polypeptides are useful as protein engineering tool for enzymatic cleavage of fusion proteins. Also provided are kits comprising the polypeptides of the invention.Type: GrantFiled: October 4, 2007Date of Patent: September 6, 2011Assignee: National University Corporation Hokkaido UniversityInventors: Katsueki Ogiwara, Takayuki Takahashi
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Patent number: 8008057Abstract: The present invention relates to methods for producing variants of a parent JP170 subtilase and of a parent BPN? subtilase and to JP170 and BPN? variants having altered properties as compared to the parent JP170/BPN? subtilase.Type: GrantFiled: March 10, 2009Date of Patent: August 30, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Stefan Minning
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Patent number: 7977081Abstract: A nucleic acid coding for pro-carboxypeptidase B (Pro-CPB), comprising three segments A, B and C, wherein at least one of the segments has one of the sequences according to SEQ ID No. 1, 2 or 3.Type: GrantFiled: September 26, 2005Date of Patent: July 12, 2011Assignee: Merck Biosciences AGInventors: Joop Van Den Heuvel, Joerg Bartuch, Arno Cordes
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Patent number: 7951574Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: December 1, 2010Date of Patent: May 31, 2011Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
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Patent number: 7951573Abstract: The present invention relates to novel subtilases from wild-type strains of Bacillus, especially the Bacillus strains ZI344, EP655, P203, EP63, ZI120, ZI130, ZI1342 and ZI140, and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry detergent or an automatic dishwashing detergent.Type: GrantFiled: November 18, 2010Date of Patent: May 31, 2011Assignee: Novozymes A/SInventors: Preben Nielsen, Martin Borchert
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Patent number: 7910349Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: September 10, 2010Date of Patent: March 22, 2011Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
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Patent number: 7906310Abstract: The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g., in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).Type: GrantFiled: May 15, 2009Date of Patent: March 15, 2011Assignee: Novozymes A/SInventors: Peter Rahbek Oestergaard, Leonardo De Maria
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Patent number: 7906309Abstract: The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75%, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86% amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.Type: GrantFiled: April 17, 2001Date of Patent: March 15, 2011Assignee: Dyadic International (USA), Inc.Inventors: Mark Aaron Emalfarb, Peter Jan Punt, Cornelia Maria Johanna Van Zeijl
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Patent number: 7901918Abstract: The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.Type: GrantFiled: January 9, 2009Date of Patent: March 8, 2011Assignee: Novozymes A/SInventors: Mads Norregaard-Madsen, Peter Rahbek Ostergaard, Claus Bo Voge Christensen, Soren Flensted Lassen
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Patent number: 7892808Abstract: The invention relates to a novel 3D structure determined for a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an altered temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention further relates to methods of generating and preparing protease variants having different properties.Type: GrantFiled: October 8, 2004Date of Patent: February 22, 2011Assignee: Norozymes A/SInventors: Leonardo De Maria, Carsten Andersen, Lars Lehmann Hylling Christensen, Soren Flensted Lassen, Peter Rahbek Ostergaard
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Patent number: 7888093Abstract: The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.Type: GrantFiled: October 31, 2003Date of Patent: February 15, 2011Assignee: Novozymes A/SInventors: Henriette Draborg, Peter Kamp Hansen, Mads Eskelund Bjornvad, Mads Norregaard-Madsen, Mikael Mikkelsen
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Patent number: 7875445Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: February 23, 2010Date of Patent: January 25, 2011Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
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Patent number: 7867747Abstract: The present invention relates to a novel protease, a polynucleotide encoding the protease, and a fibrinolytic agent comprising the same. The protease is obtained from a new gene source by using metagenomic library technology, and can replace the conventional fibrinolytic agent.Type: GrantFiled: November 8, 2006Date of Patent: January 11, 2011Assignee: Korea Ocean Research and Development InstituteInventors: Sang-Hyeon Lee, Dong-Geun Lee, Jeong-Ho Jeon, Nam-Young Kim, Jung-Hyun Lee, Sang-Jin Kim, Min-Kyung Jang
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Patent number: 7863032Abstract: The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.Type: GrantFiled: February 9, 2009Date of Patent: January 4, 2011Assignees: Novozymes, Inc., Novozymes A/SInventors: Randy Berka, Michael Rey, Preethi Ramaiya, Jens Tønne Andersen, Michael Dolberg Rasmussen, Peter Bjarke Olsen
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Patent number: 7858360Abstract: The disclosure relates to a filamentous fungal cell in which an endogenous alkaline protease activity, an endogenous neutral metalloprotease activity, an endogenous serine protease activity, and an endogenous kexin maturase activity have been completely or partially inactivated. Particularly the endogenous alkaline protease activity, the endogenous.neutral metalloprotease activity, the endogenous serine protease activity, and the kexin maturase activity are encoded by the alp, npI, pepC and kexB genes respectively. The filamentous fungal cell is particularly suitable for production of heterologous proteins such as antibodies.Type: GrantFiled: October 17, 2006Date of Patent: December 28, 2010Assignee: Novozymes A/SInventors: Mogens Trier Hansen, Christian Isak Jorgensen, Jan Lehmbeck
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Patent number: 7858354Abstract: The present invention relates to novel subtilases from wild-type strains of Bacillus, especially the Bacillus strains ZI344, EP655, P203, EP63, ZI120, ZI130, ZI1342 and ZI140, and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry detergent or an automatic dishwashing detergent.Type: GrantFiled: January 30, 2009Date of Patent: December 28, 2010Assignee: Novozymes A/SInventors: Preben Nielsen, Martin Borchert
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Patent number: 7847077Abstract: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.Type: GrantFiled: July 16, 2007Date of Patent: December 7, 2010Assignee: Martek Biosciences CorporationInventors: James G. Metz, Craig A. Weaver, William R. Barclay, James H. Flatt
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Patent number: 7842799Abstract: To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like. Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.Type: GrantFiled: September 19, 2006Date of Patent: November 30, 2010Assignees: Noda Institute for Scientific Research, Kikkoman CorporationInventors: Osamu Hatamoto, Genryou Umitsüki, Masayuki Machida, Motoaki Sano, Akimitsu Tanaka, Chitoshi Oka, Hiroshi Maeda, Hitoshi Tainaka, Touru Ito, Tomomi Uchikawa, Tsutomu Masuda, Kenichiro Matsushima
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Patent number: 7842796Abstract: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.Type: GrantFiled: March 22, 2007Date of Patent: November 30, 2010Assignee: Martek Biosciences CorporationInventors: James G. Metz, James H. Flatt, Jerry M. Kuner, William R. Barclay
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Patent number: 7838266Abstract: Disclosed herein are modified proaerolysin (PA) peptide. In some examples, the proteins include a prostate-specific protease cleavage site and can further include a prostate-tissue-specific binding domain which functionally replaces the native PA binding domain. In other examples, the proteins include a furin cleavage site and a prostate tissue-specific binding domain which functionally replaces the native PA binding domain. Methods of using such peptides to treat prostate cancer are also disclosed.Type: GrantFiled: May 27, 2010Date of Patent: November 23, 2010Assignees: University of Victoria Innovation and Development Corporation, The Johns Hopkins UniversityInventors: Samuel R. Denmeade, John T. Isaacs, J. Thomas Buckley