Patents Examined by Yong Pak
  • Patent number: 8962298
    Abstract: Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.
    Type: Grant
    Filed: April 30, 2007
    Date of Patent: February 24, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Vasantha Nagarajan, Charles E. Nakamura, Jean-Francois Tomb
  • Patent number: 8951766
    Abstract: Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.
    Type: Grant
    Filed: January 18, 2013
    Date of Patent: February 10, 2015
    Assignee: BASF Enzymes LLC
    Inventors: Janne Samuli Kerovuo, Sylke Haremza, Oliver Koch, Tilo Habicher, Dan E. Robertson, Grace Desantis, Ryan McCann, Peter Luginbuhl
  • Patent number: 8945864
    Abstract: The object of the present invention is to provide a method of determining 1,5-anhydroglucitol, including using (a) a protein which consists of the amino acid sequence of SEQ ID NO: 2; (b) a protein which consists of an amino acid sequence having deletion, substitution and/or addition of one or more amino acid residues in the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity; or (c) a protein which consists of an amino acid sequence having a homology of at least 60% with the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity.
    Type: Grant
    Filed: June 22, 2007
    Date of Patent: February 3, 2015
    Assignees: Ikeda Food Research Co., Ltd., Nippon Kayaku Kabushiki Kaisha
    Inventors: Emi Ishimaru, Hirokazu Sanada, Hironori Omura, Hideki Yoshioka, Shuhei Tsukamoto, Minoru Masuda
  • Patent number: 8945899
    Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Grant
    Filed: December 15, 2009
    Date of Patent: February 3, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • Patent number: 8911981
    Abstract: Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: December 16, 2014
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • Patent number: 8906667
    Abstract: A method of increasing cellular NADPH levels by expressing one or more genes that encode an enzyme that causes the production of NADPH. The system is combined with other enzymes that require NADPH, thus improving the overall yield of the desired product.
    Type: Grant
    Filed: August 29, 2007
    Date of Patent: December 9, 2014
    Assignee: William Marsh Rice University
    Inventors: Ka-Yiu San, George Bennett, Henry Lin, Irene Martinez, Jiangfeng Zhu
  • Patent number: 8906663
    Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
    Type: Grant
    Filed: November 16, 2012
    Date of Patent: December 9, 2014
    Assignee: Verenium Corporation
    Inventors: David Weiner, Ellen Chi, Jennifer Ann Chaplin, Aileen Milan, Jay M. Short, Grace DeSantis, Mark Madden, Mark J. Burk, Dan E. Robertson
  • Patent number: 8895307
    Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
    Type: Grant
    Filed: July 16, 2012
    Date of Patent: November 25, 2014
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • Patent number: 8889126
    Abstract: Methods of treating or preventing axonal degradation in neuropathic diseases in mammals are disclosed. The methods can comprise administering to the mammal an effective amount of an agent that acts by increasing sirtuin activity in diseased and/or injured neurons. The methods can also comprise administering to the mammal an effective amount of an agent that acts by increasing NAD activity in diseased and/or injured neurons. Also disclosed are methods of screening agents for treating a neuropathies and recombinant vectors for treating or preventing neuropathies.
    Type: Grant
    Filed: May 28, 2010
    Date of Patent: November 18, 2014
    Assignee: Washington University
    Inventors: Jeffrey Milbrandt, Toshiyuki Araki, Yo Sasaki
  • Patent number: 8883467
    Abstract: The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: November 11, 2014
    Assignee: Codexis, Inc.
    Inventors: Robert McDaniel, Behnaz Behrouzian, Louis Clark, Douglas Hattendorf, Fernando Valle
  • Patent number: 8871456
    Abstract: The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs recognized by lipoic acid ligase and lipoic acid ligase mutants.
    Type: Grant
    Filed: February 24, 2012
    Date of Patent: October 28, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Alice Y. Ting, Marta Fernandez Suarez, Hemanta Baruah
  • Patent number: 8865438
    Abstract: A process for the preparation of a monovalent succinate salt includes: a) fermenting a carbohydrate source to succinic acid by means of a micro-organism, b) adding a alkaline earth metal hydroxide, carbonate and/or hydrogencarbonate, the alkaline earth metal being calcium or magnesium, as neutralising agent during the fermentation in an aqueous medium and causing the formation of calcium succinate or magnesium succinate, c) reacting the alkaline earth metal succinate salt in an aqueous medium with a monovalent hydroxide, carbonate and/or hydrogencarbonate base to form an alkaline earth metal hydroxide, carbonate and/or hydrogencarbonate and a monovalent succinate salt, d) separating the monovalent succinate salt from the alkaline earth metal hydroxide, carbonate and/or hydrogencarbonate, and e) recycling the alkaline earth metal hydroxide, carbonate and/or hydrogencarbonate to step b.
    Type: Grant
    Filed: December 2, 2009
    Date of Patent: October 21, 2014
    Assignee: Purac Biochem B.V.
    Inventors: Jan Van Krieken, Jan Van Breugel
  • Patent number: 8859251
    Abstract: The invention describes novel pharmaceutical compositions for the treatment of virus infections and cancer. The pharmaceutical compositions include mutant oligoadenylate synthetases (OAS) that have either enhanced cell permeability, reduced oxidative potential, improved antiviral activity, improved enzymatic activity, or absent enzymatic activity. The pharmaceutical compositions have improved drug properties and retain or have enhanced antiviral activity relative to their native forms. The pharmaceutical compositions further include chemically modified oligoadenylate synthetases, such chemical modifications being designed to increase serum stability and reduce immunogenicity in vivo. Such chemical modifications further increase drug stability and manufacturability in vitro. Compositions composed of more than ninety novel modifications are described. Also described are antibodies to polypeptides of the invention.
    Type: Grant
    Filed: July 1, 2013
    Date of Patent: October 14, 2014
    Assignee: Kineta Two, LLC
    Inventors: Shawn P. Iadonato, Charles L. Magness, Mark Branum, Maralee McVean, Christina Scherer
  • Patent number: 8852584
    Abstract: The bacteriolytic effect of a bacterial cell wall lytic enzyme can be increased by the addition of a surfactant to the enzyme. As a result, the time required for lysis of cariogenic bacterium with the bacterial cell wall lytic enzyme can be shortened, and the practical utility of the bacterial cell wall lytic enzyme (such as automutanolysin) as a prophylactic or therapeutic agent for dental caries can be improved.
    Type: Grant
    Filed: July 25, 2006
    Date of Patent: October 7, 2014
    Assignee: Two Cells Co., Ltd.
    Inventors: Motoyuki Sugai, Hitoshi Komatsuzawa
  • Patent number: 8846358
    Abstract: In a step of contacting an organic material including formic acid ions and a carbon source other than the formic acid ions with a microorganism having a formate dehydrogenase gene and a hydrogenase gene under an anaerobic condition, concentration of the formic acid ions in the organic material is set to be not less than 0.01 mol/L and not more than 0.5 mol/L, and concentration of the carbon source is set to not less than 0.1 mmol/L and not more than 200 mmol/L. This allows continuously producing hydrogen for a long time, without dropping the ability of the microorganism to produce hydrogen.
    Type: Grant
    Filed: May 5, 2009
    Date of Patent: September 30, 2014
    Assignees: Sharp Kabushiki Kaisha, Research Institute of Innovative Technology for the Earth
    Inventors: Akihito Yoshida, Hideaki Yukawa, Masayuki Inui
  • Patent number: 8828704
    Abstract: Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein. Specifically disclosed herein are the use of coenzyme B12-independent butanediol dehydratases that catalyzes the substrate to product conversion of 2,3-butanediol to 2-butanone in the process of producing 2-butanol and 2-butanone.
    Type: Grant
    Filed: April 29, 2008
    Date of Patent: September 9, 2014
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Gail K. Donaldson, Andrew C. Eliot, Lori Ann Maggio-Hall, Vasantha Nagarajan, Charles E. Nakamura, Jean-Francois Tomb
  • Patent number: 8828703
    Abstract: Proteins including engineered sequences which inhibit proteases are disclosed, including proteins having two or more engineered Kunitz domains, and uses of such proteins.
    Type: Grant
    Filed: December 27, 2006
    Date of Patent: September 9, 2014
    Assignee: Dyax Corp.
    Inventor: Robert C. Ladner
  • Patent number: 8802401
    Abstract: The disclosure relates to engineered P450 polypeptides and use of such polypeptides in chemoenzymatic methods to construct selectively protected carbohydrates, which are useful as building blocks for preparation of carbohydrate derivatives and oligosaccharides
    Type: Grant
    Filed: June 18, 2008
    Date of Patent: August 12, 2014
    Assignees: The California Institute of Technology, The Scripps Research Institute
    Inventors: Frances H. Arnold, Chi-Huey Wong, Yuuichi Mitsuda, Michael M. Chen, Clay Bennett, William Greenberg, Jared Crawford Lewis, Sabine Bastian
  • Patent number: 8778658
    Abstract: The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.
    Type: Grant
    Filed: July 13, 2012
    Date of Patent: July 15, 2014
    Assignee: Verdezyne, Inc.
    Inventors: Stephen Picataggio, Tom Beardslee
  • Patent number: 8778651
    Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
    Type: Grant
    Filed: November 12, 2012
    Date of Patent: July 15, 2014
    Assignee: Verenium Corporation
    Inventors: Kelvin Wong, Jay M. Short, Mark Burk, Grace DeSantis, Robert Farwell, Kelly Chatman