Abstract: A chemiluminescent assay method and compositions are described which use a dialkyl-substituted dioxetane which is deprotected to trigger a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with two nonspirofused alkyl groups which can be triggered by a reagent to generate light are disclosed. Dialkyl-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.

Abstract: A method and compositions including a 1,2-dioxetane and a fluorescent compound is described. In particular, enzymatic triggering of a triggerable 1,2-dioxetane admixed with a surfactant and the fluorescent compound attached to a hydrocarbon to provide a co-surfactant in a micelle or other structure providing close association of these molecules is described. The method and compositions are useful in immunoassays and in DNA probes used for various purposes.

Abstract: A method and compositions including a 1,2-dioxetane and a fluorescent compound is described. In particular, enzymatic triggering of a triggerable 1,2-dioxetane admixed with a surfactant and the fluorescent compound attached to a hydrocarbon to provide a co-surfactant in a micelle or other structure providing close association of these molecules is described. The method and compositions are useful in immunoassays and in DNA probes used for various purposes.

Abstract: A chemiluminescent assay method and compositions are described which use a dialkyl-substituted dioxetane which is deprotected to trigger a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with two nonspirofused alkyl groups which can be triggered by a reagent to generate light are disclosed. Dialkyl-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.

Abstract: A method and compositions including a 1,2-dioxetane and a fluorescent compound is described. In particular, enzymatic triggering of a triggerable 1,2-dioxetane admixed with a surfactant and the fluorescent compound attached to a hydrocarbon to provide a co-surfactant in a micelle or other structure providing close association of these molecules is described. The method and compositions are useful in immunoassays and in DNA probes used for various purposes.

Abstract: Alkene precursors for preparing dialkyl-substituted dioxetanes. The dioxetanes are useful for the detection of triggering agents including enzymes.

Abstract: Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. Compositions comprising a dioxetane with two or more water-solubilizing groups, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.

Abstract: Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Dioxetanes further instituted with two or more water-solubilizing groups disposed on the dioxetane structure provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. Compositions comprising a dioxetane with two or more water-solubilizing groups, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.

Abstract: Novel light producing 1,2-dioxetanes are described of the formula ##STR1## wherein ArOX is an aryl ring substituted with an X oxy group and A are passive organic groups which allow the 1,2-dioxetane to produce light when triggered by removing X. X is a chemically labile group which is removed by an activating agent. The 1,2-dioxetane compounds can be triggered to produce light at room temperatures.

Abstract: Novel alkene intermediates useful in the preparation of 1,2-dioxetanes.

Abstract: 4-(3-beta-D-galactopyranosylphenyl)-4-methoxyspiro[1,2-dioxetane-3,2'-adama ntane] which is useful in various chemiluminescent assays.

Abstract: Improved assay methods utilizing stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility are provided. Assays are performed by a capsule chemistry analytical assay method wherein fluid capsules comprising discrete aqueous segments containing either a chemiluminescent dioxetane reagent or an activating agent separated by an oil-based isolating fluid are flowed through a conduit, and subsequently reacted to produce light.The improvement comprises using a dioxetane substituted with two or more water-solubilizing groups disposed on the dioxetane structure to provide the chemiluminescence. Compositions comprising such a dioxetane, a non-polymeric surfactant enhancer and optionally a fluorescer, provide enhanced chemiluminescence and eliminate the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems.

Abstract: This invention relates to production of singlet oxygen and includes means and methods for storage and retrieval thereof as well as continuous production from ground state oxygen. With respect to storage and retrieval of singlet oxygen, certain aromatic acceptor compounds are oxidized to yield solid endoperoxides which, in turn, are decomposed to yield singlet oxygen and regenerate the acceptor. Continuous production of singlet oxygen occurs through irradiation of dye sensitizers with electromagnetic radiation causing activation thereof and excitation of flowing ground state oxygen.

Abstract: Polymer-bound photosensitizing catalysts are effective in the heterogeneous catalysis of various photosensitized chemical reactions, such as photooxidation reactions, photodimerization reactions, and photocycloaddition reactions, and are thereafter readily separated from the reaction medium and reaction products by simple filtration. The polymer-bound photosensitizing catalysts comprise the nucleophilic displacement reaction product of (A) a polymeric material having attached thereto an available leaving group which is capable of being displaced in a nucleophilic displacement reaction with a nucleophilic reagent and (B) a photosensitizing catalytic compound containing in its molecule a nucleophile which is capable of displacing the leaving group attached to the polymeric material in a nucleophilic displacement reaction.