Abstract: The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Abstract: The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradaic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradaic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included, for collecting the microbial cells adjacent to a liquid interface formed by the cushioning liquid, upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed to prior to extraction of the pretreated sample.

Abstract: Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included, for collecting the microbial cells adjacent to a liquid interface formed by the cushioning liquid, upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed to prior to extraction of the pretreated sample.

Abstract: Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.

Abstract: The present invention provides a microfluidic devices and methods of use thereof for the concentration and capture of cells. A pulsed non-Faradic electric field is applied relative to a sample under laminar flow, which results to the concentration and capture of charged analyte. Advantageously, pulse timing is selected to avoid problems associated with ionic screening within the channel. At least one of the electrodes within the channel is coated with an insulating layer to prevent a Faradic current from flowing in the channel. Under pulsed application of a unipolar voltage to the electrodes, charged analyte within the sample is moved towards one of the electrodes via a transient electrophoretic force.

Abstract: The present invention provides a bar-code driven, completely automated, microplate-based analyzer system for performing chemical, biochemical or biological assays. The analyzer is a modular, bench-top instrument that compactly integrates subsystems for sample dispensing, liquid handling, microplate transport, thermal incubation, vortexing, solid phase separation and optical reading. An internal processor is included for automating the instrument, and a user interface to facilitate communication with the operator via a touch-sensitive liquid-crystal display (LCD), and communicating with a remote network via multiple protocols. The analyzer includes firmware resident within the processing system and the user interface allows the operator to select pre-defined assay batch protocols and the user interface is configured in such as way so as to restrict an operator from programming the firmware.

Abstract: The present invention provides methods and devices for optical performance monitoring using co-located switchable fiber Bragg gratings (FBGs) in conjunction with a few detectors. This arrangement combines the merit of both tunable FBG filters and discrete detectors to achieve high spectral resolution, fast detection process and great dynamic range. By using parallel co-located FBGs in conjunction with a banded architecture, the tuning range of each FBG becomes much smaller (equivalent of ˜0.08% strain). As a result, not only does the update speed of the spectral characteristics for each channel become much faster, but it effectively eliminates the concern of FBG long-term reliability as well.

Abstract: The present invention provides a bar-code driven, completely automated, microplate-based analyzer system for performing chemical, biochemical or biological assays. The analyzer is a modular, bench-top instrument that compactly integrates subsystems for sample dispensing, liquid handling, microplate transport, thermal incubation, vortexing, solid phase separation and optical reading. An internal processor is included for automating the instrument, and a user interface to facilitate communication with the operator via a touch-sensitive liquid-crystal display (LCD), and communicating with a remote network via multiple protocols. The analyzer includes firmware resident within the processing system and the user interface allows the operator to select pre-defined assay batch protocols and the user interface is configured in such as way so as to restrict an operator from programming the firmware.

Abstract: The present invention provides a method of conducting an assay for the detection of a target analyte with enhanced sensitivity, dynamic range, detection limit, selectivity and accuracy using a sandwich assay format. A liquid sample is first brought into contact with a solid phase, where the solid phase is coated with receptors that have a high affinity for an analyte that may be present in the sample. After an incubation period in which the analyte binds to the receptors, and is thereby immobilized onto the solid phase, a colloidal solution of dye particles is introduced. The dye particles are coated with a second type of receptor that also has a high affinity for the analyte, but a low affinity for the first receptor and also a low affinity for the solid phase. The dye particles therefore bind to the analyte and become immobilized onto the solid phase. The solid phase is then separated from the liquid phase, which in turn separates the bound dye particles from the unbound dye particles.

Abstract: The present invention provides methods and devices for optical performance monitoring using co-located switchable fiber Bragg gratings (FBGs) in conjunction with a few detectors. This arrangement combines the merit of both tunable FBG filters and discrete detectors to achieve high spectral resolution, fast detection process and great dynamic range. By using parallel co-located FBGs in conjunction with a banded architecture, the tuning range of each FBG becomes much smaller (equivalent of ˜0.08% strain). As a result, not only does the update speed of the spectral characteristics for each channel become much faster, but it effectively eliminates the concern of FBG long-term reliability as well.

Abstract: An optical performance monitor having a tunable fiber grating sandwiched between the first and second reflective surfaces of a fiber etalon provides high sensitivity, while simultaneously monitoring an optical data signal. Individual channels within the optical data signal are monitored by tuning the fiber grating over the wavelength range of the optical data signal to measure an intensity of each channel. As the tunable grating scans the wavelength range of interest, it simultaneously changes the resonant cavity length of the etalon, thus allowing the etalon to effectively monitor the tunable grating with a wavelength outside the wavelength range of the optical data signal.

Abstract: The present invention describes a method and devices for scaling the capability of a re-configurable add/drop multiplexer while not affecting the express traffic that goes through it. The invention allows field upgradability of re-configurable add/drop multiplexers with pre-selected wavelengths or wavebands such that other wavelengths or bands can be added or removed without service interruptions. This will then allow for a pay-as-you-grow model and therefore alleviate the need for having costly OADMs with a large number of addressable channels. In addition, in one of the embodiments we will describe a method for removing single point of failure through design, which is highly desirable from a network maintenance perspective.

Abstract: A method to actuate a fiber-Bragg grating (FBG) using piezoelectric actuators is disclosed. In this method, the displacement of the piezoelectric actuator will induce a strain in the FBG, resulting in a shift of the Bragg wavelength (&lgr;Bragg). A suitable application of this device is in optical communications for the controlled switching or filtering of a channel within a specified bandwidth. The piezoelectric actuator is driven by applying effective voltage versus time profiles to the piezoelectric actuator coupled to a fiber Bragg grating to vary strain in the fiber portion containing the grating in order to switch the selected wavelengths of light reflected and/or transmitted by the fiber-Bragg grating.

Abstract: The present invention relates to a method and devices for multiplexing and demultiplexing optical signals. The present invention relates to a method and devices for integrating the multiplexer and demultiplexer functionality with the switch array. The wavelength switchable optical filter comprises an optical waveguide having a grating along a section thereof, the grating comprising a pre-selected number of spaced sub-gratings with each sub-grating having a sub-grating period, the grating has a spectral response characterized by a pre-selected number of spaced band-pass windows spanning a pre-selected wavelength range. The optical filter includes modifying means connected to the optical waveguide for modifying the sub-grating period, refractive index or a combination thereof for selectively opening or closing each of said band pass windows independently of all the other band pass windows.

Abstract: An optical performance monitor having a tunable fiber grating sandwiched between the first and second reflective surfaces of a fiber etalon provides high sensitivity, while simultaneously monitoring an optical data signal. Individual channels within the optical data signal are monitored by tuning the fiber grating over the wavelength range of the optical data signal to measure an intensity of each channel. As the tunable grating scans the wavelength range of interest, it simultaneously changes the resonant cavity length of the etalon, thus allowing the etalon to effectively monitor the tunable grating with a wavelength outside the wavelength range of the optical data signal.

Abstract: The method and apparatus forming the present invention allows for rapid analysis of the optical spectrum and its content by controlling the spectral position of a channel select element and using a ratiometric wavelength and power determination technique to accurately analyze the transmitted or elected portion of the optical spectrum. The channel select element can be either a transmissive or reflective device which can be tuned by way of a control signal. The system can be operated in either scan and post process mode or in a continuous feedback mode where the position of the channel select element is determined by the ratiometric wavelength determination system and used to control the wavelength position of the channel select element. The ratiometric wavelength and power determination system, in its typical form, is based on dividing the spectrally encoded optical signal into two parts.

Abstract: The present invention provides a method and apparatus for controlling the detailed strain field imposed on a guided-wave Bragg grating. The spectral and temporal form of radiant energy traveling down optical fibers is important in many fields, including communications and sensing. The spectral distribution and center wavelength of short optical pulses is of particular importance. In the communication field the information carrying capacity of an optical fiber can be limited by the dispersion experienced by short pulses as they propagate along an optical fiber. Chirped fiber optic intracore Bragg gratings can be used to compensate for this dispersion. However, a grating with a different degree of chirp would be required for each particular communication system.

Abstract: The present invention discloses a method for demodulating, processing and multiplexing the signals from an array of structurally integrated Bragg grating laser sensors in an optoelectronic element in order that this sensing information can be transmitted from the structure by means of a single channel and a user friendly interconnect or free-space propagation. The invention also discloses a method for determining the wavelength of narrowband optical signals such as arises from Bragg grating laser sensors and includes, for each sensor, processing the optical signal through a spectral filter with a spectral transmission function that can be accurately translated by a control parameter which has been calibrated with respective to wavelength. The filtered signal is compared to the reference signal at different values of the control parameter to determine a value of the control parameter where a predetermined relationship of the spectrally filtered signal and the reference signal is achieved.