Abstract: Systems and methods using EMR and other directed energies emitted at surfaces, objects and body parts, to kill, neutralize, eliminate, remove or otherwise treat or influence objects, species, dirt, weeds, worms, pests, bacteria, viruses, parasites, fungi, other pathogens and other organic and inorganic matter from target surfaces are disclosed. One embodiment includes selecting an at least one first set of parameters of directed energy, wherein the at least one first set of parameters may include one or more of a wavelength, frequency, amplitude, duration, acceleration, deceleration, pulsation, and pulsation period; emitting the directed energy based on the selected at least one first set of parameters at a target surface; measuring in real-time the temperature of the target surface; and pausing the emitting of directed energy once a trigger occurs. This process may be repeated with different sets of parameters and directed energies and carried out in series or simultaneously.