Patents by Inventor Aaron Bensimon
Aaron Bensimon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7985542Abstract: The present invention relates to a method of detection of the presence of at least one domain of interest on a macromolecule to test, wherein said method comprises the following steps: a) determining beforehand at least two target regions on the domain of interest, designing and obtaining corresponding labeled probes of each target region, named set of probe of the domain of interest, the position of these probes one compared to the others being chosen and forming the specific signature of said domain of interest on the macromolecule to test; b) after spreading of the macromolecule to test on which the probes obtained in step a) are bound, detection of the position one compared to the others of the probes bound on the linearized macromolecule, the detection of the signature of a domain of interest indicating the presence of said domain of interest on the macromolecule to test, and conversely the absence of detection of signature or part of signature of a domain of interest indicating the absence of said doType: GrantFiled: September 7, 2006Date of Patent: July 26, 2011Assignees: Institut Pasteur, Genomic VisionInventors: Ronald Lebofsky, Aaron Bensimon, Pierre Walrafen
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Publication number: 20110159490Abstract: Eukaryotic genomes are duplicated by the activation of multiple bidirectional origins of replication. The replication programs of these cells depend on the temporal and spatial organisation of replication origins throughout the genome. To investigate the replication program in a higher eukaryote, we employed a technique called molecular combing. This technique allows for a quantitative analysis of DNA replication on a genome wide basis. As a model system, Xenopus laevis sperm chromatin were differentially labelled at successive time points after the beginning of DNA synthesis. Genomic DNA was then extracted and combed on a glass surface. Direct measurements made on the labelled DNA provided a comprehensive analysis of the spatial and temporal organisation of the X. laevis early embryo replication program and revealed that the number of replication origins activated per kilobase increases throughout the period of DNA synthesis.Type: ApplicationFiled: July 6, 2010Publication date: June 30, 2011Inventors: Aaron Bensimon, John Herrick, Olivier Hyrien
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Patent number: 7754425Abstract: The subject of the present invention is a process for aligning a macromolecule (macromolecules) on the surface S of a support, characterized in that the triple line S/A/B (meniscus) resulting from the contact between a solvent A and the surface S and a medium B is caused to move on the said surface S, the said macromolecules having a part, especially an end, anchored on the surface S, the other part, especially the other end, being in solution in the solvent A. The subject of the present invention is also a process for detecting, measuring the intramolecular distance of, separating and/or assaying a macromolecule in a sample in which a process of alignment according to the invention is used.Type: GrantFiled: May 4, 2006Date of Patent: July 13, 2010Assignees: Institut Pasteur, Centre National de la Recherche Scientifique (CRNS)Inventors: David Bensimon, Aaron Bensimon, François Heslot
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Patent number: 7732143Abstract: The invention concerns a method for detecting or locating one or several genes of one or several specific A DNA sequence or one or several molecules reacting with DNA on a B DNA characterised in that it consists in: (a) fixing and combing a certain amount of said B DNA on a combing surface; (b) reacting the product of the B combing with one or several probes, linked with the gene(s) or specific A DNA sequences, or with the molecules capable of reacting with DNA; (c) extracting information corresponding to at least one of the following categories: (1) the position of the probes, (2) the distance between the probes, (3) the size of the probes (the total sum of sizes for quantifying the number of hybridised probes) for determining therefrom the presence, the location and/or the amount of genes or specific A DNA sequences. This method can be used in particular for the diagnosis of genetic diseases.Type: GrantFiled: March 18, 2008Date of Patent: June 8, 2010Assignees: Institut Pasteur, Centre National de la Recherche Scientifique (CNRS)Inventors: Aaron Bensimon, David Bensimon, Xavier Michalet
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Publication number: 20100074533Abstract: A method of detecting a curvilinear object of a noisy image. The method includes filtering the noisy image in accordance with a two dimensional line profile. The line profile is selected within a class of steerable filters.Type: ApplicationFiled: April 14, 2008Publication date: March 25, 2010Applicants: INSTITUT PASTEUR, GENOMIC VISIONInventors: Sylvain Berlemont, Jean-Christophe Olivo-Marin, Aaron Bensimon
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Publication number: 20100041036Abstract: The present invention relates to a method of detection of the presence of at least one domain of interest on a macromolecule to test, wherein said method comprises the following steps: a) determining beforehand at least two target regions on the domain of interest, designing and obtaining corresponding labeled probes of each target region, named set of probe of the domain of interest, the position of these probes one compared to the others being chosen and forming the specific signature of said domain of interest on the macromolecule to test; b) after spreading of the macromolecule to test on which the probes obtained in step a) are bound, detection of the position one compared to the others of the probes bound on the linearized macromolecule, the detection of the signature of a domain of interest indicating the presence of said domain of interest on the macromolecule to test, and conversely the absence of detection of signature or part of signature of a domain of interest indicating the absence of said domaiType: ApplicationFiled: September 5, 2007Publication date: February 18, 2010Applicants: INSTITUT PASTEUR, GENOMIC VISIONInventors: Ronald Lebofsky, Aaron Bensimon, Pierre Walrafen
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Publication number: 20090175541Abstract: A method of detecting a curvilinear object of a noisy image. The method includes filtering the noisy image in accordance with a two dimensional line profile. The line profile is selected within a class of steerable filters. A beamlet coefficient is calculated in accordance with the filtering, wherein a coefficient above a predetermined threshold identifies a local feature.Type: ApplicationFiled: April 14, 2008Publication date: July 9, 2009Applicants: INSTITUT PASTEUR, GENOMIC VISIONInventors: Aaron Bensimon, Jean-Christophe Olivo-Marin, Sylvain Berlemont
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Publication number: 20090123926Abstract: The invention concerns a method for detecting or locating one or several genes of one or several specific A DNA sequence or one or several molecules reacting with DNA on a B DNA characterised in that it consists in: (a) fixing and combing a certain amount of said B DNA on a combing surface; (b) reacting the product of the B combing with one or several probes, linked with the gene(s) or specific A DNA sequences, or with the molecules capable of reacting with DNA; (c) extracting information corresponding to at least one of the following categories: (1) the position of the probes. (2) the distance between the probes, (3) the size of the probes (the total sum of sizes for quantifying the number of hybridised probes) for determining therefrom the presence, the location and/or the amount of genes or specific A DNA sequences. This method can be used in particular for the diagnosis of genetic diseases.Type: ApplicationFiled: March 18, 2008Publication date: May 14, 2009Inventors: Aaron Bensimon, David Bensimon, Xavier Michalet
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Patent number: 7368234Abstract: A method for detecting or locating one or several genes of one or several specific A DNA sequence or one or several molecules reacting with DNA on a B DNA where the method can include: (a) fixing and combing a certain amount of the B DNA on a combing surface; (b) reacting the product of step (a) with one or several probes, linked with the gene(s) or specific A DNA sequences, or with the molecules capable of reacting with DNA; (c) extracting information corresponding to at least one of the following categories: (1) the position of the probes, (2) the distance between the probes, (3) the size of the probes (the total sum of sizes for quantifying the number of hybridized probes) and determining from this extracted information the presence, the location and/or the amount of a gene(s) or specific A DNA sequences. This method can be used in particular for the diagnosis of genetic diseases.Type: GrantFiled: March 11, 2003Date of Patent: May 6, 2008Assignee: Institut PasteurInventors: Aaron Bensimon, David Bensimon, Xavier Michalet
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Publication number: 20080064114Abstract: The present invention relates to a method of detection of the presence of at least one domain of interest on a macromolecule to test, wherein said method comprises the following steps: a) determining beforehand at least two target regions on the domain of interest, designing and obtaining corresponding labeled probes of each target region, named set of probe of the domain of interest, the position of these probes one compared to the others being chosen and forming the specific signature of said domain of interest on the macromolecule to test; b) after spreading of the macromolecule to test on which the probes obtained in step a) are bound, detection of the position one compared to the others of the probes bound on the linearized macromolecule, the detection of the signature of a domain of interest indicating the presence of said domain of interest on the macromolecule to test, and conversely the absence of detection of signature or part of signature of a domain of interest indicating the absence of said doType: ApplicationFiled: September 7, 2006Publication date: March 13, 2008Inventors: Ronald Lebofsky, Aaron Bensimon, Pierre Walrafen
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Publication number: 20060257910Abstract: The subject of the present invention is a process for aligning a macromolecule (macromolecules) on the surface S of a support, characterized in that the triple line S/A/B (meniscus) resulting from the contact between a solvent A and the surface S and a medium B is caused to move on the said surface S, the said macromolecules having a part, especially an end, anchored on the surface S, the other part, especially the other end, being in solution in the solvent A. The subject of the present invention is also a process for detecting, measuring the intramolecular distance of, separating and/or assaying a macromolecule in a sample in which a process of alignment according to the invention is used.Type: ApplicationFiled: May 4, 2006Publication date: November 16, 2006Inventors: David Bensimon, Aaron Bensimon, Francois Heslot
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Patent number: 7122647Abstract: A product includes a surface on which a coupled complex is aligned, adherent, and stationary, obtained by a process that includes providing a support having a surface with a molecule with biological activity attached, contacting the surface with a sample containing a macromolecule to couple the macromolecule to the molecule on the surface, passing a meniscus over the coupled complex to align the coupled complex, and detecting the macromolecule in the coupled complex. The product is alternatively obtained by passing a meniscus over the molecule with biological activity prior to contacting the surface with a sample containing a macromolecule to couple the macromolecule to the molecule on the surface.Type: GrantFiled: February 13, 2003Date of Patent: October 17, 2006Assignees: Institut Pasteur, Centre National de la Recherche ScientifiqueInventors: David Bensimon, Aaron Bensimon, François Heslot
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Patent number: 7052650Abstract: Apparatus for the manipulating and testing of molecules and in particular of DNA comprising a surface on which the molecule is anchored on multiple points at one end and a paramagnetic bead on which said molecule is anchored on multiple points at its other end, magnetic means for applying a force to the bead, said magnetic means being used to control the stretching and rotation of said bead and molecule, optical magnification means and a camera for the visualisation of said bead, computer means to which the images of the camera are transmitted, said computer means comprising means for analyzing the motions of the bead. A method for the manipulating and testing of molecules and in particular of DNA in which a molecule is anchored at one end to a fixed surface and at its other end to a paramagnetic bead wherein said molecule is anchored on multiple points at each of said ends.Type: GrantFiled: June 6, 2002Date of Patent: May 30, 2006Assignee: Center National de la Recherche Scientifique (CNRS)Inventors: Terrence R. Strick, Jean F. Allemand, David Bensimon, Aaron Bensimon, Vincent Croquette
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Publication number: 20050142593Abstract: Eukaryotic genomes are duplicated by the activation of multiple bidirectional origins of replication. The replication programs of these cells depend on the temporal and spatial organisation of replication origins throughout the genome. To investigate the replication program in a higher eukaryote, we employed a technique called molecular combing. This technique allows for a quantitative analysis of DNA replication on a genome wide basis. As a model system. Xenopus Laevis sperm chromatin were differentially labelled at successive time points after the beginning of DNA synthesis. Genomic DNA was then extracted and combed on a glass surface. Direct measurements made on the labelled DNA provided a comprehensive analysis of the spatial and temporal organisation of the X. leavis early embryo replication program and revealed that the number of replication origins activated per kilobase increases throughout the period of DNA synthesis.Type: ApplicationFiled: November 5, 2004Publication date: June 30, 2005Inventors: Aaron Bensimon, John Herrick, Olivier Hyrien
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Publication number: 20050144473Abstract: The invention concerns a device containing a personalizable substrate associated to a reader allowing the conditional operation of an electric or electronic appliance after comparison of the data with a memory by an identification and comparison circuit located on an essential part of said appliance. The invention also discloses a method of use under conditional operation of electric or electronic appliances.Type: ApplicationFiled: October 25, 2004Publication date: June 30, 2005Inventors: Aaron Bensimon, Armand Berneman, David Bensimon, Danielle Berneman
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Patent number: 6809628Abstract: A device containing a personalizable substrate associated to a reader allowing the conditional operation of an electric or electronic appliance after comparison of the data with a memory by an identification and comparison circuit located on an essential part of said appliance and methods of using the device under conditional operation of electric or electronic appliances.Type: GrantFiled: March 29, 2000Date of Patent: October 26, 2004Inventors: Aaron Bensimon, Armand Berneman, David Bensimon, Danielle Berneman
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Publication number: 20040033510Abstract: The invention concerns a method for detecting or locating one or several genes of one or several specific A DNA sequence or one or several molecules reacting with DNA on a B DNA characterised in that it consists in: (a) fixing and combing a certain amount of said B DNA on a combing surface; (b) reacting the product of the B combing with one or several probes, linked with the gene(s) or specific A DNA sequences, or with the molecules capable of reacting with DNA; (c) extracting information corresponding to at least one of the following categories: (1) the position of the probes, (2) the distance between the probes, (3) the size of the probes (the total sum of sizes for quantifying the number of hybridised probes) for determining therefrom the presence, the location and/or the amount of genes or specific A DNA sequences. This method can be used in particular for the diagnosis of genetic diseases.Type: ApplicationFiled: March 11, 2003Publication date: February 19, 2004Applicant: Institut Pasteur and Centre National de la Recherche Scientifique (CNRS).Inventors: Aaron Bensimon, David Bensimon, Xavier Michalet
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Publication number: 20030175779Abstract: The subject of the present invention is a process for aligning a macromolecule (macromolecules) on the surface S of a support, characterized in that the triple line S/A/B (meniscus) resulting from the contact between a solvent A and the surface S and a medium B is caused to move on the said surface S, the said macromolecules having a part, especially an end, anchored on the surface S, the other part, especially the other end, being in solution in the solvent A.Type: ApplicationFiled: February 13, 2003Publication date: September 18, 2003Applicant: Institut Pasteur and Centre National de la Recherche ScientifiqueInventors: David Bensimon, Aaron Bensimon, Francois Heslot
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Patent number: 6548255Abstract: The subject of the present invention is a process for aligning a macromolecule (macromolecules) on the surface S of a support, characterized in that the triple line S/A/B (meniscus) resulting from the contact between a solvent A and the surface S and a medium B is caused to move on the said surface S, the said macromolecules having a part, especially an end, anchored on the surface S, the other part, especially the other end, being in solution in the solvent A. The subject of the present invention is also a process for detecting, measuring the intramolecular distance of, separating and/or assaying a macromolecule in a sample in which a process of alignment according to the invention is used.Type: GrantFiled: June 13, 2001Date of Patent: April 15, 2003Assignee: Institut Pasteur and Centre National de la Recherche ScientifiqueInventors: David Bensimon, Aaron Bensimon, François Heslot
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Publication number: 20020048767Abstract: The invention concerns a method for detecting or locating one or several genes of one or several specific A DNA sequence or one or several molecules reacting with DNA on a B DNA characterized in that it consists in. (a) fixing and combing a certain amount of said B DNA on a combing surface; (b) reacting the product of the B combing with one or several probes, linked with the gene(s) or specific A DNA sequences. or with the molecules capable of reacting with DNA; (c) extracting information corresponding to at least one of the following categories: (1) the position of the probes. (2) the distance between the probes, (3) the size of the probes (the total sum of size quantifying the number of hybridised probes) for determining there from the presence, the location and/or the amount of genes or specific A DNA sequences. This method can be used in particular for the diagnosis of genetic diseases.Type: ApplicationFiled: August 3, 2001Publication date: April 25, 2002Applicant: Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)Inventors: Aaron Bensimon, David Bensimon, Xavier Michalet