Abstract: The present disclosure relates to various different types of mutations or modifications in Saccharomyces cerevisiae coding and noncoding regions leading to enhanced cellobiohydrolase I production for, e.g., supplements and nutraceuticals.

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.

Abstract: In accordance with one embodiment of the present invention, a host bus adapter for implementing a first port of a node on a network of nodes interconnected through an intelligent connection system comprising: a transceiver providing a physical interface for the first port; and a protocol controller that transmits a notification to the intelligent connection system on behalf of a remote second port indicating that the second port may have failed when the second port fails to communicate with the first port.