Abstract: A flow cytometer apparatus is provided herein including (a) a flow cell, (b) a fluidic pathway having a second end coupled to a first end of the flow cell, (c) a probe coupled to a first end of the fluidic pathway, (d) a sensor configured to detect one or more properties of a fluid in the fluidic pathway and positioned between the probe and the first end of the flow cell, (e) a processor in communication with the sensor, and (f) a non-transitory computer readable medium having stored therein instructions that are executable to cause the processor to perform functions including: (i) receiving, via the processor, the one or more properties of the fluid in the fluidic pathway detected by the sensor, and (ii) determining, based on the detected one or more properties of the fluid in the fluidic pathway, a presence of a separation gas in the fluid in the fluidic pathway.

Abstract: A flow cytometer apparatus is provided herein including (a) a flow cell, (b) a fluidic pathway having a second end coupled to a first end of the flow cell, (c) a probe coupled to a first end of the fluidic pathway, (d) a sensor configured to detect one or more properties of a fluid in the fluidic pathway and positioned between the probe and the first end of the flow cell, (e) a processor in communication with the sensor, and (f) a non-transitory computer readable medium having stored therein instructions that are executable to cause the processor to perform functions including: (i) receiving, via the processor, the one or more properties of the fluid in the fluidic pathway detected by the sensor, and (ii) determining, based on the detected one or more properties of the fluid in the fluidic pathway, a presence of a separation gas in the fluid in the fluidic pathway.

Abstract: A method for detecting a separation gas in a fluid flow stream is provided herein. In one example, a voltage output signal is generated by a scatter detector of a flow cytometer as a flow stream of a plurality of gas-separated samples passes through the flow cytometer. The voltage output signal is sampled, and a timestamp and a voltage value are recorded for each sampled voltage of the voltage output signal that is greater than a separation gap threshold. In some examples the separation gap threshold is at least two times greater than a maximum voltage output of the samples. Flow cytometry systems including software configured to perform these method steps are also described.

Abstract: A method for detecting a separation gas in a fluid flow stream is provided herein. In one example, a voltage output signal is generated by a scatter detector of a flow cytometer as a flow stream of a plurality of gas-separated samples passes through the flow cytometer. The voltage output signal is sampled, and a timestamp and a voltage value are recorded for each sampled voltage of the voltage output signal that is greater than a separation gap threshold. In some examples the separation gap threshold is at least two times greater than a maximum voltage output of the samples. Flow cytometry systems including software configured to perform these method steps are also described.