Abstract: A system and method for producing high levels of autologous IL-1RA cytokine, includes a blood collection vessel, a cover, a portion of separation gel, an anticoagulant portion, a plasma collection syringe comprising a sharp needle, a buffy coat collection syringe and an incubation tube with cover. The blood collection vessel is adapted such that when containing whole blood and being centrifuged, after treatment yields separation fractions comprising, a first fraction of RBCs sediment, a second fraction of said gel, a third fraction comprising WBCs, platelets and a fourth fraction of plasma solution.

Abstract: A system and a method are disclosed for obtaining a plasma composition enriched with bioactive molecules such as growth factors and cytokines and depleted in contaminants such as viruses.

Abstract: A system for obtaining plasma enriched in platelets is disclosed which is closed to the atmosphere. The system includes: a collection tube containing an anticoagulant portion and a separation gel; a first collection syringe adapted to collect a portion of fluid relatively depleted in platelets from said collection tube after centrifugation; and a second collection syringe adapted to collect plasma enriched in platelets from said collection tube after centrifugation, said second collection syringe further comprising a filter unit adapted to filter cells included in said plasma enriched in platelets.

Abstract: A system for obtaining plasma enriched in platelets is disclosed which is closed to the atmosphere. The system includes: a collection tube containing an anticoagulant portion and a separation gel; a first collection syringe adapted to collect a portion of fluid relatively depleted in platelets from said collection tube after centrifugation; and a second collection syringe adapted to collect plasma enriched in platelets from said collection tube after centrifugation, said second collection syringe further comprising a filter unit adapted to filter cells included in said plasma enriched in platelets.

Abstract: A system and method for producing high levels of autologous IL-1RA cytokine, includes a blood collection vessel, a cover, a portion of separation gel, an anticoagulant portion, a plasma collection syringe comprising a sharp needle, a buffy coat collection syringe and an incubation tube with cover. The blood collection vessel is adapted such that when containing whole blood and being centrifuged, after treatment yields separation fractions comprising, a first fraction of RBCs sediment, a second fraction of said gel, a third fraction comprising WBCs, platelets and a fourth fraction of plasma solution.

Abstract: A system for obtaining plasma enriched in platelets is disclosed which is closed to the atmosphere. The system includes: a collection tube containing an anticoagulant portion and a separation gel; a first collection syringe adapted to collect a portion of fluid relatively depleted in platelets from said collection tube after centrifugation; and a second collection syringe adapted to collect plasma enriched in platelets from said collection tube after centrifugation, said second collection syringe further comprising a filter unit adapted to filter cells included in said plasma enriched in platelets.

Abstract: A system and method for producing high levels of autologous IL-1RA cytokine, includes a blood collection vessel, a cover, a portion of separation gel, an anticoagulant portion, a plasma collection syringe comprising a sharp needle, a buffy coat collection syringe and an incubation tube with cover. The blood collection vessel is adapted such that when containing whole blood and being centrifuged, after treatment yields separation fractions comprising, a first fraction of RBCs sediment, a second fraction of said gel, a third fraction comprising WBCs, platelets and a fourth fraction of plasma solution.

Abstract: A system and method for producing high levels of autologous IL-1RA cytokine, includes a blood collection vessel, a cover, a portion of separation gel, an anticoagulant portion, a plasma collection syringe comprising a sharp needle, a buffy coat collection syringe and an incubation tube with cover. The blood collection vessel is adapted such that when containing whole blood and being centrifuged, after treatment yields separation fractions comprising, a first fraction of RBCs sediment, a second fraction of said gel, a third fraction comprising WBCs, platelets and a fourth fraction of plasma solution.

Abstract: The present invention relates to a system and method for producing high levels of autologous IL-1RA cytokine, comprising: a blood collection vessel (1), a cover (4), a portion of separation gel (2), an anticoagulant portion (3), a plasma collection syringe comprising a sharp needle (9), a buffy coat collection syringe (11) and an incubation tube with cover (15). The blood collection vessel is adapted such that, when containing the whole blood (5), and centrifuged after treatment, yields separation fractions comprising, a first fraction of RBCs sediment (6), a second fraction of said gel (2), a third fraction comprising WBCs, platelets and a fourth fraction of plasma solution (8).

Abstract: A system for obtaining plasma enriched in platelets is disclosed which is closed to the atmosphere. The system includes: a collection tube containing an anticoagulant portion and a separation gel; a first collection syringe adapted to collect a portion of fluid relatively depleted in platelets from said collection tube after centrifugation; and a second collection syringe adapted to collect plasma enriched in platelets from said collection tube after centrifugation, said second collection syringe further comprising a filter unit adapted to filter cells included in said plasma enriched in platelets.

Abstract: The invention relates to a system and method for obtaining a cellular fraction enriched with defined cells effective in promoting a defined cellular response from an in vivo cellular sample. The system is closable to the atmosphere, and includes a cell suspension collection tube containing gel and anticoagulant, adapted such that, when containing cell suspension and centrifuged after treatment the collection tube yields separation fractions, a first fraction comprising cellular fluid, a second fraction comprising gel layer, a third fraction comprising enriched cellular portion on top of the gel layer and a fourth fraction comprising a cell poor portion. The cell poor portion may be extracted and discarded prior to extracting the enriched cellular portion.

Abstract: The present invention relates to a system and method for producing high levels of autologous IL-1RA cytokine, comprising: a blood collection vessel (1), a cover (4), a portion of separation gel (2), an anticoagulant portion (3), a plasma collection syringe comprising a sharp needle (9), a buffy coat collection syringe (11) and an incubation tube with cover (15). The vessel is adapted such that, when containing the whole blood (5), and centrifuged after treatment, yields separation fractions comprising, a first fraction of RBCs sediment (6), a second fraction of said gel (2), a third fraction comprising WBCs, platelets and), and fourth fraction of plasma solution (8).

Abstract: The invention relates to a system and method for obtaining a cellular fraction enriched with defined cells effective in promoting a defined cellular response from an in vivo cellular sample comprising: a collection tube for collecting a cell suspension (1), an anticoagulant portion (4), a portion of separation gel (3), a first collection syringe for collecting a cell depleted fluid (10), a second collection syringe for collecting the enriched cellular portion (11) and at least one needle (1O) for attaching to each syringe. The system is closable to the atmosphere, further wherein the cell suspension collection tube contains the gel (3) and the anticoagulant (4), the tube is adapted such that, when containing cell suspension and centrifuged after treatment yields separation fractions, a first fraction comprising cellular fluid (6), a second fraction comprises gel layer (3), a third fraction comprises enriched cellular portion (7) on top of the gel layer and a fourth fraction comprises a cell poor portion (8).

Abstract: An assembly comprising: (a) a test tube for receiving a blood sample or a platelet-comprising fraction thereof and for obtaining therein, by a procedure that includes centrifugation, a platelet rich plasma (PRP) fraction; (b) an elongated filter device having a first and second end and a lumen extending there between, and having a filter fitted at the first end, the filter having a effective pore size such to permit passage of platelets and not permit passage of white blood cells (WBC) larger than a defined size into the lumen, the second end having an opening; the first end of the elongated filter device being adapted for tight fitting into said test tube such that when forced into the test tube to exert pressure on the PRP fraction, the PRP fraction with the platelets filters into said lumen and white blood cells of a size large than said defined size remain in the test tube to thereby obtain a WBC-selective PRP within said lumen.

Abstract: An assembly comprising: (a) a test tube for receiving a blood sample or a platelet-comprising fraction thereof and for obtaining therein, by a procedure that includes centrifugation, a platelet rich plasma (PRP) fraction; (b) an elongated filter device having a first and second end and a lumen extending there between, and having a filter fitted at the first end, the filter having a effective pore size such to permit passage of platelets and not permit passage of white blood cells (WBC) larger than a defined size into the lumen, the second end having an opening; the first end of the elongated filter device being adapted for tight fitting into said test tube such that when forced into the test tube to exert pressure on the PRP fraction, the PRP fraction with the platelets filters into said lumen and white blood cells of a size large than said defined size remain in the test tube to thereby obtain a WBC-selective PRP within said lumen.

Abstract: A closure for sealing a sample tube and a method for establishing vacuum conditions within the tube while the closure is loosely positioned on the tube are provided. The closure comprises: (a) an inner stopper portion for insertion into the tube; and (b) an outer skirt portion for enclosing the tube, the skirt portion including at least two radially inwardly directed extensions for loosely positioning the closure on the tube, the extensions defining at least two passageways therebetween for exchanging gases between the interior of the tube and the surrounding atmosphere when the extensions are positioned on the tube. Preferably, the inner stopper portion and the outer skirt portion are integrally made.