Patents by Inventor Aaron Hummel

Aaron Hummel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230112792
    Abstract: Described herein are methods of introducing variation into plants. Also described herein are methods of producing a plant and/or seed, methods of breeding plants, methods of generating a commercially relevant trait or allele in a plant, and methods of generating a plant and/or seed library. Products produced from such methods (e.g., plants and/or seeds) are also described herein.
    Type: Application
    Filed: March 12, 2021
    Publication date: April 13, 2023
    Inventors: Aaron Hummel, David Schwark
  • Patent number: 11591607
    Abstract: This invention relates to CRISPR-Cas nucleases codon optimized for expression in plants and nucleic acid constructs encoding base editors comprising a CRISPR-Cas nuclease and a deaminase domain, wherein the nucleic acid constructs are optimized for expression in a plant. The invention further relates to methods of modifying nucleic acids using the nucleic acid constructs.
    Type: Grant
    Filed: October 23, 2020
    Date of Patent: February 28, 2023
    Assignee: Pairwise Plants Services, Inc.
    Inventors: Nathaniel Graham, Aaron Hummel, Yongjoo Kim, Joseph Matthew Watts
  • Publication number: 20220145334
    Abstract: This invention relates to recombinant nucleic constructs comprising CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.
    Type: Application
    Filed: November 5, 2021
    Publication date: May 12, 2022
    Inventors: Yongjoo Kim, Joseph Matthew Watts, Aaron Hummel, Shai Joshua Lawit, David Schwark
  • Publication number: 20210403898
    Abstract: Described herein are methods of modifying or editing a target nucleic acid such as methods that edit adenine to cytosine, thymine, or guanine. Compositions and systems for modifying or editing a target nucleic acid are also described. Methods, compositions and systems described herein may be used for generating allelic diversity.
    Type: Application
    Filed: June 30, 2021
    Publication date: December 30, 2021
    Inventors: Yongjoo Kim, Aaron Hummel
  • Publication number: 20210403901
    Abstract: The present invention relates to novel methods for discovering traits and generating cellular systems having improved phenotypes. In particular, the present invention provides methods for the development of plants having agronomically optimized phenotypes by using targeted mutagenesis with few or no off-target effects. Targeted mutagenesis is achieved by the introduction of a base editor complex or of a STEME complex comprising an array of guide RNAs targeting a nucleic acid sequence of interest. The present invention also relates to cellular systems obtained by the methods described herein and to the use of a base editor complex or the STEME complex comprising an array of guide RNAs for generating a cellular system having an agronomically important phenotype and for identification of an agronomically important phenotype.
    Type: Application
    Filed: November 4, 2019
    Publication date: December 30, 2021
    Applicant: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences
    Inventors: Yu Mei, Aaron Hummel, Zarir Vaghchhipawala, Caixia Gao, Chao Li, Rui Zhang
  • Publication number: 20210292754
    Abstract: Described herein are nonnaturally occurring nucleic acids that are and/or that provide a guide nucleic acid (e.g., a guide RNA). Further described herein are methods, compositions, systems (e.g., expression systems), and RNA structures (e.g., architectures), which may increase natural guide architecture (NGA) availability for Cas9 interactions.
    Type: Application
    Filed: March 16, 2021
    Publication date: September 23, 2021
    Inventors: Hae Jin Kim, Aaron Hummel, Shai Lawit, Aabid Shariff, Thomas Poorten, Nathaniel Graham
  • Publication number: 20210238598
    Abstract: Described herein are methods of modifying or editing a target nucleic acid such as methods that edit cytosine to thymine and adenine to guanine and/or methods that edit cytosine to thymine, adenine, or guanine. Compositions and systems for modifying or editing a target nucleic acid are also described. Methods, compositions and systems described herein may be used for generating allelic diversity.
    Type: Application
    Filed: January 29, 2021
    Publication date: August 5, 2021
    Inventors: Yongjoo Kim, Aaron Hummel
  • Publication number: 20210230616
    Abstract: The present invention relates to methods for targeted editing in a plant, a plant cell or material, which is combined with the parallel introduction of a phenotypically selectable trait. Furthermore, methods are provided not comprising a step of introducing a transgenic selection marker sequence.
    Type: Application
    Filed: May 7, 2018
    Publication date: July 29, 2021
    Applicant: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES
    Inventors: Caixia GAO, Rui ZHANG, Jinxing LIU, Aaron HUMMEL, Zarir VAGHCHHIPAWALA, Mathias LABS
  • Publication number: 20210207113
    Abstract: This invention relates to recombinant nucleic constructs comprising a sequence-specific DNA binding protein, DNA-dependent DNA polymerase and a DNA encoded repair template, optionally a DNA endonuclease or wherein the sequence-specific DNA binding protein comprises DNA endonuclease activity, and methods of use thereof for modifying nucleic acids in cells and organisms
    Type: Application
    Filed: January 6, 2021
    Publication date: July 8, 2021
    Inventors: Jingyi Nie, Aaron Hummel, Shai Joshua Lawit
  • Publication number: 20210171947
    Abstract: Described herein are recruitment methods and compounds, compositions, and systems for recruitment. Also described herein are methods and compositions for template editing of genomic DNA using Agrobacterium-derived T-DNA molecules and/or proteins.
    Type: Application
    Filed: December 6, 2020
    Publication date: June 10, 2021
    Inventors: Aaron Hummel, Shai Joshua Lawit, Jingyi Nie, Sabine Fräbel, Sharon Leigh Guffy
  • Publication number: 20210163968
    Abstract: The present invention relates to optimized systems for the genome editing of eukaryotic cells, preferably plant cells. Also provided is a plant delivery system comprising at least one Cpf1 enzyme, or a plant optimized construct encoding the same, to be synergistically combined with a Cpf1 guide RNA system. Further provided is a Cpf1 guide RNA system being flanked by a Hammerhead ribozyme sequence at the 5? end and by a plant-derived Hepatitis Delta Vims (HDV)-like ribozyme sequence at the 3? end, or being embedded within a coding or non-coding region, of a sequence encoding a frame sequence. Novel plant-derived HDV-like ribozyme sequences are also provided. Further provided are methods for improved genome editing, and the use of the various systems provided herein to obtain transformed plants, plant cells, tissues, organs, or a progeny thereof, or a plant material, modified in a targeted way even at difficult to access target sites.
    Type: Application
    Filed: January 11, 2019
    Publication date: June 3, 2021
    Applicant: KWS SAAT SE & CO. KGaA
    Inventors: Aaron HUMMEL, Zarir VAGHCHHIPAWALA
  • Publication number: 20210147862
    Abstract: This invention relates to recombinant nucleic constructs comprising a DNA binding domain, an endonuclease and a reverse transcriptase and methods of use thereof for modifying nucleic acids in plants.
    Type: Application
    Filed: October 23, 2020
    Publication date: May 20, 2021
    Inventors: Aaron Hummel, Joseph Matthew Watts, Shai Joshua Lawit, Nathaniel Graham, Larry Gilbertson, Ervin Nagy, Linda Rymarquis
  • Publication number: 20210147861
    Abstract: This invention relates to CRISPR-Cas nucleases codon optimized for expression in plants and nucleic acid constructs encoding base editors comprising a CRISPR-Cas nuclease and a deaminase domain, wherein the nucleic acid constructs are optimized for expression in a plant. The invention further relates to methods of modifying nucleic acids using the nucleic acid constructs.
    Type: Application
    Filed: October 23, 2020
    Publication date: May 20, 2021
    Inventors: Nathaniel Graham, Aaron Hummel, Yongjoo Kim, Joseph Matthew Watts
  • Publication number: 20210130835
    Abstract: This invention relates to recombinant nucleic constructs comprising Type V CRISPR-Cas effector proteins, reverse transcriptases and extended guide nucleic acids and methods of use thereof for modifying nucleic acids in plants.
    Type: Application
    Filed: November 5, 2020
    Publication date: May 6, 2021
    Inventors: Joseph Matthew Watts, Aaron Hummel, Yongjoo Kim, Shai Joshua Lawit, David Schwark
  • Publication number: 20210071189
    Abstract: The present invention relates to the targeted regulation of gene expression and more specifically to synthetic transcription factors (STFs) comprising at least one highly target specific engineered recognition domain based on a CRISPR/Cpf1 system and further comprising at least one activation or silencing domain to modulate the expression of a gene of interest, preferably to modulate the transcription of a morphogenic gene of a eukaryote, in particular a plant. Further disclosed are methods using the STFs to enhance transformation frequencies, to optimize successful genome editing approaches, to provide haploid or double haploid organisms, and/or to provide compositions suitable for general transformation, but also for breeding purposes.
    Type: Application
    Filed: December 21, 2018
    Publication date: March 11, 2021
    Applicant: KWS SAAT SE & CO. KGaA
    Inventors: Mathias LABS, Aaron HUMMEL, Yu MEI
  • Publication number: 20200407737
    Abstract: The present invention relates to the use of CRISPR/CasX systems in plants for genome engineering, and compositions used in such methods.
    Type: Application
    Filed: May 3, 2018
    Publication date: December 31, 2020
    Applicant: KWS SAAT SE & Co. KGaA
    Inventors: Aaron HUMMEL, Zarir VAGHCHHIPAWALA
  • Publication number: 20200354734
    Abstract: The present invention relates to improved methods for precision genome editing (GE), preferably in eukaryotic cells, and particularly to methods for GE in cells with specifically altered expression of Polymerase theta and altered characteristics of at least one further enzyme involved in a non-homologous end-joining (NHEJ) DNA repair pathway. Further provided are cellular systems and tools related to the methods provided. Specifically, methods are provided, wherein Polymerase theta and NHEJ blockage and/or GE are performed in a transient way so that the endogenous Polymerase theta and cellular NHEJ machinery is easily reactivated after a targeted edit, and/or without permanent integration of certain editing tools.
    Type: Application
    Filed: October 30, 2018
    Publication date: November 12, 2020
    Applicant: KWS SAAT SE & Co. KGaA
    Inventors: Markus NIESSEN, Aaron HUMMEL, Erik JONGEDIJK, Zarir VAGHCHHIPAWALA, Fridtjof WELTMEIER, René GLENZ
  • Publication number: 20200332307
    Abstract: The present invention relates to the targeted regulation of gene expression and more specifically to synthetic transcription factors (STFs) comprising at least one highly target specific engineered recognition domain and further comprising at least one activation or silencing domain to modulate the expression of a gene of interest, preferably to modulate the transcription of a morphogenic gene of a eukaryote. Further disclosed are methods using the STFs to enhance transformation frequencies, to optimize successful genome editing approaches, to provide haploid or double haploid organisms, and/or to provide compositions suitable for general transformation, but also for breeding purposes.
    Type: Application
    Filed: December 21, 2018
    Publication date: October 22, 2020
    Applicant: KWS SAAT SE & CO. KGaA
    Inventors: Aaron HUMMEL, Mathias LABS
  • Publication number: 20200255856
    Abstract: The present invention relates to materials and methods for creating and maintaining a cereal plant line for the production of a hybrid cereal plant, that include for example, and not limitation, using the BLue Aleurone (BLA) system.
    Type: Application
    Filed: August 29, 2018
    Publication date: August 13, 2020
    Applicants: KWS SAAT SE & Co. KGaA, THE UNIVERSITY OF SYDNEY, GLOBAL CROP INNOVATIONS PTY. LTD.
    Inventors: Norman DARVEY, Peng ZHANG, Richard TRETHOWAN, Chong Mei DONG, Jacob LAGE, Nicholas BIRD, Christopher TAPSELL, Aaron HUMMEL
  • Publication number: 20190352653
    Abstract: Materials and methods for conferring geminivirus resistance to plants or plant cells, and particularly to materials and methods for using CRISPR/Cas or CRISPR/Cpf1 systems to confer resistance to geminiviruses. Materials and methods are described to insert sequence at a double stranded break in a geminivirus genome.
    Type: Application
    Filed: December 20, 2017
    Publication date: November 21, 2019
    Applicant: KWS SAAT SE
    Inventors: Aaron Hummel, Derek Graham Bartlem