Abstract: Continuous compression molding machines (CCMMs) and methods of continuous compression molding a consolidated thermoplastic matrix composite material are disclosed herein. The CCMMs include a mold, a heat zone heating structure, a consolidation zone heating structure, and a stress relaxation zone heating structure. The CCMMs also include a press structure, a demold structure, and a supply structure. The methods include providing a thermoplastic matrix composite material (TMCM) that includes a thermoplastic material to a CCMM. During the providing, the methods also include heating the TMCM within a heat zone of the CCMM, cooling and consolidating the TMCM within a consolidation zone of the CCMM, relaxing stress within the TMCM within a stress relaxation zone of the CCMM, demolding the TMCM within a demold zone of the CCMM at a mold temperature that is greater than a glass transition temperature of the thermoplastic material, and periodically compressing the TMCM.

Abstract: Disclosed herein are surgical tool systems and methods of using such to install a fixator in a biological tissue. The systems are capable of accurately measuring torque and rotational velocity and providing real time feedback to a user during surgery.

Abstract: A composite skin is attached to an underlying structure by fasteners that are countersunk into the structure. The composite skin comprises a thermoplastic material that has been melted and formed into a countersink in the structure using a heated tool.

Abstract: Aspects of the present disclosure include methods for identifying a set of fluorophore-biomolecule reagent pairs for characterizing a sample by flow cytometry. Methods according to certain embodiments include calculating a spectral spillover spreading parameter for a plurality of fluorophores, pairing each fluorophore with a biomolecule that is specific for a biomarker of a cell in the sample to generate a plurality of fluorophore-biomolecule reagent pairs, generating an adjusted spillover spreading matrix for the fluorophore-biomolecule reagent pairs based on the spectral spillover spreading parameter of each fluorophore and a biomarker classification parameter and identifying an optimal set of fluorophore-biomolecule reagent pairs based on the calculated spillover spreading values from the adjusted spillover spreading matrix. Systems and non-transitory computer readable storage medium for practicing the subject methods are also provided.

Abstract: In various embodiments method are provided for generating expression cassettes and gene therapy vectors comprising those cassettes that recapitulate the spatiotemporal pattern of expression of the endogenous gene. In certain embodiments the methods comprise (i) selecting a target gene; (ii) identifying putative regulatory elements associated with the target gene; (iii) determining if the regulatory element is a key regulatory element and (iv) providing a list of the key regulatory elements identified in step (iii).

Abstract: Continuous compression molding machines (CCMMs) and methods of continuous compression molding a consolidated thermoplastic matrix composite material are disclosed herein. The CCMMs include a mold, a heat zone heating structure, a consolidation zone heating structure, and a stress relaxation zone heating structure. The CCMMs also include a press structure, a demold structure, and a supply structure. The methods include providing a thermoplastic matrix composite material (TMCM) that includes a thermoplastic material to a CCMM. During the providing, the methods also include heating the TMCM within a heat zone of the CCMM, cooling and consolidating the TMCM within a consolidation zone of the CCMM, relaxing stress within the TMCM within a stress relaxation zone of the CCMM, demolding the TMCM within a demold zone of the CCMM at a mold temperature that is greater than a glass transition temperature of the thermoplastic material, and periodically compressing the TMCM.

Abstract: Disclosed herein are surgical tool systems and methods of using such to install a fixator in a biological tissue. The systems are capable of accurately measuring torque and rotational velocity and providing real time feedback to a user during surgery.

Abstract: Continuous compression molding machines (CCMMs) and methods of continuous compression molding a consolidated thermoplastic matrix composite material are disclosed herein. The CCMMs include a mold, a heat zone heating structure, a consolidation zone heating structure, and a stress relaxation zone heating structure. The CCMMs also include a press structure, a demold structure, and a supply structure. The methods include providing a thermoplastic matrix composite material (TMCM) that includes a thermoplastic material to a CCMM. During the providing, the methods also include heating the TMCM within a heat zone of the CCMM, cooling and consolidating the TMCM within a consolidation zone of the CCMM, relaxing stress within the TMCM within a stress relaxation zone of the CCMM, demolding the TMCM within a demold zone of the CCMM at a mold temperature that is greater than a glass transition temperature of the thermoplastic material, and periodically compressing the TMCM.

Abstract: A method and structure for processing a plurality of prepreg sheets includes a conveyor assembly having a first conveyor, a second conveyor, and a processing zone positioned between the first and second conveyors. In an implementation, a plurality of prepreg sheets are advanced from a plurality of material supply creels into the processing zone by a first conveyor and a second conveyor turning in opposite directions. Within the processing zone, a compressive pressure is applied to the prepreg sheets by first pressure plates attached to first conveyor and second pressure plates attached to the second conveyor. A thermoplastic within the prepreg sheets is melted within the processing zone by a heater to consolidate the prepreg sheets into a consolidated laminate.

Abstract: An assembly to manufacture a roll of prepreg material. The assembly includes a moving system for picking a sheet of prepreg material from a stack of sheets and moving the sheet along a feed path. An alignment system aligns a leading edge of the sheets with a trailing edge of a roll of the prepreg material. A welding system connects the sheet to the roll.

Abstract: Introduced here are session management platforms able to facilitate multimedia sessions that involve host(s) and user(s). A session management platform may enable multimedia communication between the host(s) and a participating user. The users may be added into positions in a priority queue, where the priority queue may represent an order of users to be used to select a given user for participation in the multimedia session. Upon termination of a session between the host(s) and a first participating user, the priority queue may be updated to remove the first participating user from the queue and a second participating user is selected. The multimedia session may enable multimedia communication between the host(s) and the second participating user based on the second participating user being selected.

Abstract: Described herein are transcriptional relay systems useful for reducing background signal in protein expression and reporter assays. These systems utilize a nucleic acid system wherein a promoter sequence controls expression of a synthetic transcription factor that activates transcription of a reporter molecule.

Abstract: Described herein are methods and systems useful for interrogating protein-protein interactions. These systems utilize non-functional fragments of polypeptides that can complement each other to form a fully functional fragment in vivo. The fully functional polypeptide releases a transcription regulating polypeptide that can bind and activate or repress a reporter element.

Abstract: In certain embodiments a lentiviral vector having optimized reduced size LCR with improved enhancer activity is provided. In certain embodiments direct treatment of a subject by direct introduction of the vector(s) described herein is contemplated. The lentiviral compositions may be formulated for delivery by any available route including, but not limited to parenteral (e.g., intravenous), intradermal, subcutaneous, oral (e.g., inhalation), transdermal (topical), transmucosal, rectal, and vaginal.

Abstract: Aspects of the present disclosure include methods for identifying a set of fluorophore-biomolecule reagent pairs for characterizing a sample by flow cytometry. Methods according to certain embodiments include calculating a spectral spillover spreading parameter for a plurality of fluorophores, pairing each fluorophore with a biomolecule that is specific for a biomarker of a cell in the sample to generate a plurality of fluorophore-biomolecule reagent pairs, generating an adjusted spillover spreading matrix for the fluorophore-biomolecule reagent pairs based on the spectral spillover spreading parameter of each fluorophore and a biomarker classification parameter and identifying an optimal set of fluorophore-biomolecule reagent pairs based on the calculated spillover spreading values from the adjusted spillover spreading matrix. Systems and non-transitory computer readable storage medium for practicing the subject methods are also provided.

Abstract: Introduced here are session management platforms able to facilitate multimedia sessions that involve host(s) and user(s). A session management platform may enable multimedia communication between the host(s) and a participating user. The users may be added into positions in a priority queue, where the priority queue may represent an order of users to be used to select a given user for participation in the multimedia session. Upon termination of a session between the host(s) and a first participating user, the priority queue may be updated to remove the first participating user from the queue and a second participating user is selected. The multimedia session may enable multimedia communication between the host(s) and the second participating user based on the second participating user being selected.

Abstract: Introduced here are session management platforms able to facilitate multimedia sessions that involve host(s) and user(s). A session management platform may enable multimedia communication between the host(s) and a participating user. The users may be added into positions in a priority queue, where the priority queue may represent an order of users to be used to select a given user for participation in the multimedia session. Upon termination of a session between the host(s) and a first participating user, the priority queue may be updated to remove the first participating user from the queue and a second participating user is selected. The multimedia session may enable multimedia communication between the host(s) and the second participating user based on the second participating user being selected.

Abstract: A method and structure for processing a plurality of prepreg sheets includes a conveyor assembly having a first conveyor, a second conveyor, and a processing zone positioned between the first and second conveyors. In an implementation, a plurality of prepreg sheets are advanced from a plurality of material supply creels into the processing zone by a first conveyor and a second conveyor turning in opposite directions. Within the processing zone, a compressive pressure is applied to the prepreg sheets by first pressure plates attached to first conveyor and second pressure plates attached to the second conveyor. A thermoplastic within the prepreg sheets is melted within the processing zone by a heater to consolidate the prepreg sheets into a consolidated laminate.

Abstract: An assembly to manufacture a roll of prepreg material. The assembly includes a moving system for picking a sheet of prepreg material from a stack of sheets and moving the sheet along a feed path. An alignment system aligns a leading edge of the sheets with a trailing edge of a roll of the prepreg material. A welding system connects the sheet to the roll.