Abstract: The present disclosure provides materials and methods for co-determining the cellular location and nucleotide sequence of a DNA that is contacted by (or in close proximity to) a protein of interest in a single cell. Thus the present disclosure provides methods and materials wherein the cellular location of the DNA comprising a DNA-binding site or otherwise in close proximity to a protein of interest is coupled to the sequence of said DNA to provide contemporaneous imaging and sequence measurement of a protein-DNA interaction.

Abstract: The present disclosure provides materials and methods to link imaging and sequencing measurements of a single cell. Sequencing information can be linked with phenotypic measurements that are not directly encoded in the genome such as morphological features, protein expression & localization, organelle dynamics, or the metabolic composition of a cell.