Abstract: The present invention is directed to a novel method of determining inhibitors of proteolytically active coagulation factors, referred to herein as anticoagulants, in a sample, in particular the qualitative detection of direct thrombin and factor Xa inhibitors in a sample. The method of the present invention allows a qualitative determination of the nature anticoagulants present in a sample. This can be achieved with only one coagulation-based test. The method can be used in a test kit, including a point-of-care (POC) system.

Abstract: The present invention is directed to a novel method of determining inhibitors of proteolytically active coagulation factors, referred to herein as anticoagulants, in a sample, in particular the qualitative detection of direct thrombin and factor Xa inhibitors in a sample. The method of the present invention allows a qualitative determination of the nature anticoagulants present in a sample. This can be achieved with only one coagulation-based test. The method can be used in a test kit, including a point-of-care (POC) system.

Abstract: The present invention is directed to a novel method of determining inhibitors of proteolytically active coagulation factors, referred to herein as anticoagulants, in a sample, in particular the qualitative detection of direct thrombin and factor Xa inhibitors in a sample. The method of the present invention allows a qualitative determination of the nature anticoagulants present in a sample. This can be achieved with only one coagulation-based test. The method can be used in a test kit, including a point-of-care system.

Abstract: A method to increase the production of a desired chemical compound in a microorganism by introduction of a DNA sequence at the 5? end of the encoding DNA gene sequence capable of forming a stem loop and capable of increasing the stability of mRNA transcripts from one or more genes, thus stabilized mRNAs, corresponding DNA sequences and microorganisms.

Abstract: The present invention relates to a newly identified microorganisms capable of direct production of hydroxytyrosol (hereinafter also referred to as Hy-T) from a carbon source obtainable from the D-glucose metabolization pathway. The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Hy-T. The invention also relates to genetically engineered microorganisms and their use for the direct production of Hy-T.

Abstract: The present invention relates to the use of polynucleotides and polypeptides as biotechnological tools in the production of hydroxytyrosol from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of hydroxytyrosol in said microorganism. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the production of hydroxytyrosol.

Abstract: The present invention relates to newly identified microorganisms capable of direct production of hydroxytyrosol (hereinafter also referred to as Hy-T) from tyrosol. The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Hy-T from tyrosol. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the conversion of tyrosol into Hy-T.

Abstract: A method to increase the production of a desired chemical compound in a microorganism by introduction of a DNA sequence at the 5? end of the encoding DNA gene sequence capable of forming a stem loop and capable of increasing the stability of mRNA transcripts from one or more genes, thus stabilized mRNAs, corresponding DNA sequences and microorganisms.