Abstract: Examples of a microfluidic analyser (100, 200A, 200B, 200C) for in-vitro biosensing and analysis of a biological sample are described. The microfluidic analyser comprises a platform (102, 202A, 202B, 202C, 402A, 402B, 500) to hold at least one cartridge (300) carrying a biological sample and at least one reagent. The microfluidic analyser includes a fluid control unit (108, 1000) having needles (110, 1002, 1102) to pierceably connect with sealed ends (304) of the cartridge to establish a fluid connection with the cartridge, and a pneumatic unit (112, 1004, 1202) to provide at least one of a positive pressure and a negative pressure to the cartridge. The microfluidic analyser includes an optical unit (104, 600) comprising an optical sensor (124, 604, 800) to detect presence of a fluorescence biomarker in biological sample held in the cartridge.