Abstract: The present disclosure provides materials and methods for producing droplet libraries with thousands of distinct reagents. The monodispersed libraries prepared according to the methods provided herein use multiwall plates and are compatible with microfluidic processing.

Abstract: The present disclosure provides materials and methods for partitioning cells and high throughput, single-cell epigenetic sequencing. Methods for using chemically-modified hydrogels are also provided herein. Wherein determining the epigenomic state of a single-cell comprises preparing a functionalized hydrogel, wherein said functionalized hydrogel is chemically modified with a functional group capable of binding to a capture reagent.

Abstract: Existing spatial transcriptomics technologies require dead tissues and are not compatible with live cell cultures. The present disclosure provides materials and methods for sequencing a single cell from a cell culture sample and obtaining morphologic or phenotypic measurements and information by combining sequencing approaches and spatial hashing (e.g., barcoding) at a single cell level.

Abstract: Provided herein are systems, compositions, and methods for uniquely identifying and analyzing nucleic acid molecules. In particular, provided herein are systems, compositions, and methods employing modified base identifiers to generate a unique pattern in individual nucleic acid molecules and analyzing the modified molecules, or copies thereof, to identify or differentiate the molecules.

Abstract: The present disclosure provides materials and methods for contemporaneously analyzing genotype and phenotype from frozen specimens, the present disclosure can be extended to any biologic context where obtaining nucleic acid and protein information from single nuclei is valuable. As used herein, the method is termed nuclear DAb-seq (nDAb-seq).

Abstract: The present disclosure provides materials and methods for obtaining multiple measurements from the same sample. For example, the present disclosure provides methods to perform spatial metabolomics (using, for example, mass spectrometry imaging), transcriptomics and fluorescent imaging of the same tissue section.

Abstract: The present disclosure provides materials and methods for partitioning cells and high throughput, single-cell multi-omic sequencing. Methods for pathogen detection and identification, microbiome analysis, personalized medicine, environmental analysis where single-cell information is critical are each provided herein.

Abstract: Testing devices are disclosed. In accordance with a first implementation, a testing device includes a sample chamber is to receive a sample and a reagent reservoir that contains a reagent used to determine a presence of a target molecule in the sample. A sample fluidic line is fluidically coupled to the sample chamber and a common fluidic line and a reagent fluidic line is fluidically coupled to the reagent reservoir and to the common fluidic line. A diagnostic indicator is coupled to the common fluidic line. The sample and the reagent flow through the respective sample fluidic line and the reagent fluidic line toward the common fluidic line and form a mixture within the common fluidic line and the common fluidic line enables a threshold incubation period of the mixture prior to the mixture flowing to the diagnostic indicator.

Abstract: The present disclosure provides materials and methods for sequencing a tissue sample and that allows spatial information about the tissue to be recovered by sequencing approaches at a single cell level.

Abstract: The present disclosure provides materials and methods for detecting the presence of a target, such as a nucleic acid, biomolecule or biological particle, or virus genome in a sample. The present disclosure further provides point of care detection methods that cane done quickly and simply, optionally without sophisticated instrumentation. The present disclosure further provides improved methods for detecting and quantifying detection signals from various emulsions.

Abstract: The present invention generally pertains to a system for performing injection of multiple substantially controlled volumes into or out of a droplet, and methods and kits comprising the same. The system of the present invention comprises at least one microfluidic channel, one or more injection channels, an injection inlet associated with each of the one or more injection channels, and a mechanism for disrupting an interface between a droplet and a fluid and/or emulsion, wherein the at least one microfluidic channel comprises one or more droplets are flowing therein, and wherein each of the one or more injection channels comprises at least one fluid and/or emulsion therein.

Abstract: The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein.

Abstract: The present invention generally pertains to a system for performing injection of multiple substantially controlled volumes into or out of a droplet, and methods and kits comprising the same. The system of the present invention comprises at least one microfluidic channel, one or more injection channels, an injection inlet associated with each of the one or more injection channels, and a mechanism for disrupting an interface between a droplet and a fluid and/or emulsion, wherein the at least one microfluidic channel comprises one or more droplets are flowing therein, and wherein each of the one or more injection channels comprises at least one fluid and/or emulsion therein.

Abstract: The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein.

Abstract: The present invention generally pertains to a system for performing injection of multiple substantially controlled volumes into or out of a droplet, and methods and kits comprising the same. The system of the present invention comprises at least one microfluidic channel, one or more injection channels, an injection inlet associated with each of the one or more injection channels, and a mechanism for disrupting an interface between a droplet and a fluid and/or emulsion, wherein the at least one microfluidic channel comprises one or more droplets are flowing therein, and wherein each of the one or more injection channels comprises at least one fluid and/or emulsion therein.

Abstract: The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein.

Abstract: The present invention generally pertains to a system for performing injection of multiple substantially controlled volumes into or out of a droplet, and methods and kits comprising the same. The system of the present invention comprises at least one microfluidic channel, one or more injection channels, an injection inlet associated with each of the one or more injection channels, and a mechanism for disrupting an interface between a droplet and a fluid and/or emulsion, wherein the at least one microfluidic channel comprises one or more droplets are flowing therein, and wherein each of the one or more injection channels comprises at least one fluid and/or emulsion therein.

Abstract: Methods and systems for controlling fluid flow through a cartridge for use in an assay of a fluid sample for detection of a target analyte. In one aspect, the cartridge includes a microfluidic device having a network of microfluidic channels, one or more reservoirs and one or more actuatable valves for controlling fluid flow from the reservoirs through the microfluidic channels. In some embodiments, the valve includes a deformable membrane movable between a closed configuration in which the membrane seals against the microfluidic device inhibiting fluid flow and an open configuration moved away from the microfluidic device to allow fluid flow through the microfluidic channels. Other valve embodiments utilize frangible membranes or user-removable membrane.

Abstract: The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein.