Patents by Inventor Adam E. Cohen
Adam E. Cohen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10457715Abstract: Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants.Type: GrantFiled: November 28, 2016Date of Patent: October 29, 2019Assignees: President and Fellows of Harvard College, The Governors of the University of AlbertaInventors: Adam E. Cohen, Daniel Hochbaum, Peng Zou, Samouil Leon Farhi, Robert Earl Campbell, Yongxin Zhao, Daniel Jed Harrison
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Patent number: 10392426Abstract: The invention, in some aspects relates to light-activated ion channel polypeptides and encoding nucleic acids and also relates in part to compositions comprising light-activated ion channel polypeptides and methods using light-activated ion channel polypeptides to alter cell activity and function.Type: GrantFiled: February 6, 2015Date of Patent: August 27, 2019Assignees: Massachusetts Institute of Technology, The Governors of the University of Alberta, President and Fellows of Harvard CollegeInventors: Nathan Klapoetke, Edward Boyden, Yongku Cho, Brian Y. Chow, Gane K. S. Wong, Adam E. Cohen, Daniel R. Hochbaum
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Patent number: 10077463Abstract: Provided herein are photochemical crosslinkers and photocleavable crosslinkers and their uses in methods for cell selection from cell cultures. The photochemical crosslinkers comprise a fluorescent dye and a radical generator. The photocleavable crosslinkers comprise a photocleavable linker linking two electrophilic groups to each other. Also provided are systems for imaging cells comprising a plurality of cells crosslinked to extracellular matrix proteins using a crosslinker as described, an imaging apparatus, an illuminating apparatus, and software for image processing.Type: GrantFiled: January 14, 2016Date of Patent: September 18, 2018Assignee: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Miao-Ping Chien
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Patent number: 10048193Abstract: A curved surface is placed tangent to a slide and displaces a sample liquid from the point or line of contact outward. Imaging indicates a region where fluorescence is observed, and the location of the fluorescence indicates the molecular size. The radius of curvature of the lens is known, the distance from the (center) point of contact of the observed fluorescence is measured with a microscope and the distance of the lens surface to the slide's surface can then be calculated. This distance represents the size of the molecule or ensemble of molecules emitting. Similarly, absorbance, etc. could be measured with a light source below the slide.Type: GrantFiled: August 11, 2016Date of Patent: August 14, 2018Assignee: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Sabrina R. Leslie
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Patent number: 9863880Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.Type: GrantFiled: November 17, 2014Date of Patent: January 9, 2018Assignee: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, Ali Kabiri, Jason W. Sickler, Brett J. Gyarfas, Jeremy Lackey, Gerard Schmid, Benjamin Cipriany, Jack Jewell, Lawrence West, Michael Ferrigno, Paul E. Glenn, Adam E. Cohen, Anthony Bellofiore
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Publication number: 20170313757Abstract: Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants.Type: ApplicationFiled: November 28, 2016Publication date: November 2, 2017Applicants: President and Fellows of Harvard College, The Governors of the University of AlbertaInventors: Adam E. Cohen, Daniel Hochbaum, Peng Zou, Samouil Leon Farhi, Robert Earl Campbell, Yongxin Zhao, Daniel Jed Harrison
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Patent number: 9791455Abstract: The invention provides methods, cells and constructs for optical measurement of membrane potential. These methods can be used in cells that are not accessible to presently available methods using electrodes. The methods can be directed to, for example, high-throughput drug screening assays to determine agents that can affect membrane potential of a target cell.Type: GrantFiled: June 15, 2015Date of Patent: October 17, 2017Assignee: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Joel Kralj, Adam D. Douglass
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Patent number: 9702874Abstract: The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.Type: GrantFiled: November 16, 2015Date of Patent: July 11, 2017Assignee: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Joel Kralj, Adam D. Douglass, Daniel Hochbaum
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Publication number: 20170174730Abstract: The invention, in some aspects relates to light-activated ion channel polypeptides and encoding nucleic acids and also relates in part to compositions comprising light-activated ion channel polypeptides and methods using light-activated ion channel polypeptides to alter cell activity and function.Type: ApplicationFiled: March 1, 2017Publication date: June 22, 2017Applicants: Massachusetts Institute of Technology, The Governors of the University of Alberta, President and Fellows of Harvard CollegeInventors: Nathan Klapoetke, Edward Boyden, Yongku Cho, Brian Y. Chow, Gane K.S. Wong, Adam E. Cohen, Daniel R. Hochbaum
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Publication number: 20170160188Abstract: A curved surface is placed tangent to a slide and displaces a sample liquid from the point or line of contact outward. Imaging indicates a region where fluorescence is observed, and the location of the fluorescence indicates the molecular size, The radius of curvature of the lens is known, the distance from the (center) point of contact of the observed fluorescence is measured with a microscope and the distance of the lens surface to the slide's surface can then be calculated. This distance represents the size of the molecule or ensemble of molecules emitting. Similarly, absorbance, etc. could be measured with a light source below the slide.Type: ApplicationFiled: August 11, 2016Publication date: June 8, 2017Inventors: Adam E. COHEN, Sabrina R. LESLIE
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Patent number: 9518103Abstract: Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants.Type: GrantFiled: June 17, 2015Date of Patent: December 13, 2016Assignees: President and Fellows of Harvard College, The Governors of the University of AlbertaInventors: Adam E. Cohen, Daniel Hochbaum, Peng Zou, Samouil Leon Farhi, Robert Earl Campbell, Yongxin Zhao, Daniel Jed Harrison
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Publication number: 20160208308Abstract: Provided herein are photochemical crosslinkers and photocleavable crosslinkers and their uses in methods for cell selection from cell cultures. The photochemical crosslinkers comprise a fluorescent dye and a radical generator. The photocleavable crosslinkers comprise a photocleavable linker linking two electrophilic groups to each other. Also provided are systems for imaging cells comprising a plurality of cells crosslinked to extracellular matrix proteins using a crosslinker as described, an imaging apparatus, an illuminating apparatus, and software for image processing.Type: ApplicationFiled: January 14, 2016Publication date: July 21, 2016Applicant: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Miao-Ping Chien
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Publication number: 20160069876Abstract: The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.Type: ApplicationFiled: November 16, 2015Publication date: March 10, 2016Applicant: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Joel Kralj, Adam D. Douglass, Daniel Hochbaum
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Publication number: 20150369740Abstract: Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants.Type: ApplicationFiled: June 17, 2015Publication date: December 24, 2015Applicants: President and Fellows of Harvard College, The Governors of the University of AlbertaInventors: Adam E. Cohen, Daniel Hochbaum, Peng Zou, Samouil Leon Farhi, Robert Earl Campbell, Yongxin Zhao, Daniel Jed Harrison
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Patent number: 9207237Abstract: The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.Type: GrantFiled: June 12, 2014Date of Patent: December 8, 2015Assignee: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Joel Kralj, Adam D. Douglass, Daniel Hochbaum
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Publication number: 20150293336Abstract: A spatial filter includes a first focal plane to receive sample fluorescence and auto-fluorescence from a microscope, a first lens to receive the sample fluorescence and auto-fluorescence and focus rays of the sample fluorescence, a mask aperture positioned in a plane where sample fluorescence rays maximally converge, the mask aperture positioned where such rays converge to pass the rays, the aperture having a size that is a function of characteristics of the microscope, and a second lens positioned to receive the passed rays from the spatial filter and form images at a second focal plane to couple to a camera.Type: ApplicationFiled: October 30, 2013Publication date: October 15, 2015Inventors: Adam E. Cohen, Jennifer H. Hou
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Publication number: 20150285820Abstract: The invention provides methods, cells and constructs for optical measurement of membrane potential. These methods can be used in cells that are not accessible to presently available methods using electrodes. The methods can be directed to, for example, high-throughput drug screening assays to determine agents that can affect membrane potential of a target cell.Type: ApplicationFiled: June 15, 2015Publication date: October 8, 2015Applicant: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Joel Kralj, Adam D. Douglass
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Publication number: 20150223679Abstract: The invention, in some aspects relates to light-activated ion channel polypeptides and encoding nucleic acids and also relates in part to compositions comprising light-activated ion channel polypeptides and methods using light-activated ion channel polypeptides to alter cell activity and function.Type: ApplicationFiled: February 6, 2015Publication date: August 13, 2015Applicants: MASSACHUSETTS INSTITUTE OF TECHNOLOGY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE, THE GOVERNORS OF THE UNIVERSITY OF ALBERTAInventors: Nathan Klapoetke, Edward Boyden, Yongku Cho, Brian Y. Chow, Gane K.S. Wong, Adam E. Cohen, Daniel R. Hochbaum
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Publication number: 20150177150Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.Type: ApplicationFiled: November 17, 2014Publication date: June 25, 2015Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, Ali Kabiri, Jason W. Sickler, Brett J. Gyarfas, Jeremy Lackey, Gerard Schmid, Benjamin Cipriany, Jack Jewell, Lawrence West, Michael Ferrigno, Paul E. Glenn, Adam E. Cohen, Anthony Bellofiore
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Patent number: 9057734Abstract: The invention provides methods, cells and constructs for optical measurement of membrane potential. These methods can be used in cells that are not accessible to presently available methods using electrodes. The methods can be directed to, for example, high-throughput drug screening assays to determine agents that can affect membrane potential of a target cell.Type: GrantFiled: August 23, 2011Date of Patent: June 16, 2015Assignee: President and Fellows of Harvard CollegeInventors: Adam E. Cohen, Joel M. Kralj, Adam D. Douglass