Abstract: In one aspect, a method for detection of a target in a sample based on the change of electrochemical cyclic voltammetry peak profile is provided. The method may involve at least two or more electroactive moieties (EAMs) comprising a transitional metal complex and an anchor group. Said transitional metal complex may be capable of reversibly or semi-reversibly transferring one or more electrons. Said anchor group may allow said EAMs to covalently attach to a solid support, forming a self-assembled monolayer (SAM) on the surface. At least one EAM may further comprises a capture ligand that can be recognized and bound by a target or a surrogate target. When a sample is introduced to an EAM, the interaction between said capture ligand and said target or surrogate target may lead to change of the properties of the SAM containing all EAMs involved. Said change can be detected by an electrochemical detection system.

Abstract: The disclosure relates to novel methods for performing a solution based assay reaction with an electroactive active moiety (EAM) that subsequently forms a self-assembled monolayer (SAM) utilizing the advantages of faster solution reaction kinetics, SAM protected electrode and surface based electrochemistry for electronic measurement.

Abstract: This invention describes methods for detecting target analytes by utilizing an electroactive moiety (EAM) that functions as a substrate for a specific enzyme. If target analyte is present, a functional group is enzymatically removed from a transition metal complex resulting in quantifiable electrochemical signal at two unique potentials, E01 and E02 that is detected through a self-assembled monolayer (SAM) on an electrode.

Abstract: The disclosure relates to novel methods for performing a solution based assay reaction with an electroactive active moiety (EAM) that subsequently forms a self-assembled monolayer (SAM) utilizing the advantages of faster solution reaction kinetics, SAM protected electrode and surface based electrochemistry for electronic measurement.

Abstract: Competitive assays are provided for the detection and quantification of a target analyte utilizing a modified electro-active moiety and an enzyme, in which the target analyte and a target analog moiety are substrates. This method may be used to detect and/or quantify many classes of biological molecules and has a number of applications, e.g., in vitro diagnostic assays and devices.

Abstract: In one aspect, a method for detection of a target in a sample based on the change of electrochemical cyclic voltammetry peak profile is provided. The method may involve at least two or more electroactive moieties (EAMs) comprising a transitional metal complex and an anchor group. Said transitional metal complex may be capable of reversibly or semi-reversibly transferring one or more electrons. Said anchor group may allow said EAMs to covalently attach to a solid support, forming a self-assembled monolayer (SAM) on the surface. At least one EAM may further comprises a capture ligand that can be recognized and bound by a target or a surrogate target. When a sample is introduced to an EAM, the interaction between said capture ligand and said target or surrogate target may lead to change of the properties of the SAM containing all EAMs involved. Said change can be detected by an electrochemical detection system.

Abstract: The disclosure relates to novel methods for performing a solution based assay reaction with an electroactive active moiety (EAM) that subsequently forms a self-assembled monolayer (SAM) utilizing the advantages of faster solution reaction kinetics, SAM protected electrode and surface based electrochemistry for electronic measurement.

Abstract: This invention describes methods for detecting target analytes by utilizing an electroactive moiety (EAM) that functions as a substrate for a specific enzyme. If target analyte is present, a functional group is enzymatically removed from a transition metal complex resulting in quantifiable electrochemical signal at two unique potentials, Eo1 and Eo2 that is detected through a self-assembled monolayer (SAM) on an electrode.