Abstract: The present disclosure provides a transposon system comprising: i) a nucleotide sequence encoding polypeptides that form a CRISPR-associated transposase complex; ii) a nucleotide sequence encoding a guide RNA; and iii) a transposon, or an insertion site for a transposon, flanked by CAST complex recognition sites. The present disclosure provides a prokaryotic cell comprising a subject transposon system. The transposon system is useful for editing the genome of a target prokaryotic cell. The present disclosure provides methods for editing the genome of a target prokaryotic cell. The present disclosure further provides systems and methods for identifying, within a heterogeneous population of prokaryotic cells, prokaryotic species that are susceptible to genetic modification and gene editing.

Abstract: The present invention provides for a method for screening for gene function for a bacteriophage, the method comprising: (1) (a) providing one or more host organism, such as a species or strain, libraries, (b) providing randomly barcoded transposon sequencing (such as RB-TnSeq), and (c) screening for loss-of-function (LOF) mutant phenotypes; or (2) (a) providing one or more DNA barcoded overexpression strain libraries (such as Dub-seq) using DNA of the host organism and/or phage, and (b) screening for gain-of-function (GOF).

Abstract: The present invention provides for methods of and compositions for enhancing microbial conversion of nitrate into ammonium. The methods and compositions alter the end products of microbial nitrate reduction to improve agricultural efficiency and lead to more sustainable and economically profitable agricultural practices.

Abstract: The present invention provides for methods of and compositions for enhancing microbial conversion of nitrate into ammonium. The methods and compositions alter the end products of microbial nitrate reduction to improve agricultural efficiency and lead to more sustainable and economically profitable agricultural practices.