Patents by Inventor Adam Mefferd

Adam Mefferd has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • COMPOSITIONS FOR THE INACTIVATION OF VIRUS REPLICATION AND METHODS OF MAKING AND USING THE SAME
    Publication number: 20190351070
    Abstract: Provided herein are recombinant constructs, vectors and expression cassettes including a first promoter which is suitably a tRNA promoter operably connected to a first polynucleotide encoding a first single guide RNA and a second promoter operably connected to a second polynucleotide encoding a Cas9 polypeptide. The first single guide RNA includes a first portion complementary to a strand of a target sequence of a DNA virus and a second portion capable of interacting with the Cas9 polypeptide. Also provided are codon optimized Staphylococcus aureus derived Cas9 polynucleotides and polypeptides with nuclear localization signals and optionally an epitope tag. Also provided are constructs for production of sgRNAs including a tRNA. Methods of inhibiting viral replication, inhibiting expression of a target sequence from a virus or treating a viral infection or viral induced cancer using the compositions are also provided.
    Type: Application
    Filed: May 10, 2019
    Publication date: November 21, 2019
    Applicant: Duke University
    Inventors: Bryan R. Cullen, E. Matthew Kennedy, Hal P. Bogerd, Anand Kornepati, Adam Mefferd
  • Compositions for the inactivation of virus replication and methods of making and using the same
    Patent number: 10286084
    Abstract: Provided herein are recombinant constructs, vectors and expression cassettes including a first promoter which is suitably a tRNA promoter operably connected to a first polynucleotide encoding a first single guide RNA and a second promoter operably connected to a second polynucleotide encoding a Cas9 polypeptide. The first single guide RNA includes a first portion complementary to a strand of a target sequence of a DNA virus and a second portion capable of interacting with the Cas9 polypeptide. Also provided are codon optimized Staphylococcus aureus derived Cas9 polynucleotides and polypeptides with nuclear localization signals and optionally an epitope tag. Also provided are constructs for production of sgRNAs including a tRNA. Methods of inhibiting viral replication, inhibiting expression of a target sequence from a virus or treating a viral infection or viral induced cancer using the compositions are also provided.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: May 14, 2019
    Assignees: Duke University, Emory University, The United States of America as Represented by the Department of Veterans Affairs
    Inventors: Bryan R. Cullen, E. Matthew Kennedy, Hal P. Bogerd, Anand Kornepati, Adam Mefferd, Raymond F. Schinazi
  • COMPOSITIONS FOR THE INACTIVATION OF VIRUS REPLICATION AND METHODS OF MAKING AND USING THE SAME
    Publication number: 20170049909
    Abstract: Provided herein are recombinant constructs, vectors and expression cassettes including a first promoter which is suitably a tRNA promoter operably connected to a first polynucleotide encoding a first single guide RNA and a second promoter operably connected to a second polynucleotide encoding a Cas9 polypeptide. The first single guide RNA includes a first portion complementary to a strand of a target sequence of a DNA virus and a second portion capable of interacting with the Cas9 polypeptide. Also provided are codon optimized Staphylococcus aureus derived Cas9 polynucleotides and polypeptides with nuclear localization signals and optionally an epitope tag. Also provided are constructs for production of sgRNAs including a tRNA. Methods of inhibiting viral replication, inhibiting expression of a target sequence from a virus or treating a viral infection or viral induced cancer using the compositions are also provided.
    Type: Application
    Filed: February 18, 2015
    Publication date: February 23, 2017
    Applicants: DUKE UNIVERSITY, EMORY UNIVERSITY
    Inventors: Bryan R. Cullen, E. Matthew Kennedy, Hal P. Bogerd, Anand Kornepati, Adam Mefferd, Raymond F. Schinazi