Abstract: Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.

Abstract: Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.

Abstract: Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.

Abstract: A mounting system configured to support a component relative to a mounting surface of a vehicle includes a mounting bracket including a mounting portion configured to be coupled to the mounting surface of the vehicle. The mounting bracket defining a component receptacle configured to support the component. The mounting bracket comprises a first plastic material. The mounting system also includes a seal coupled to the mounting bracket and configured to engage the mounting surface of the vehicle to inhibit ingress of debris between the mounting portion and the mounting surface of the vehicle. The seal comprises a second plastic material, different than the first plastic material. The mounting system may be included in a window assembly where the mounting surface of the vehicle is an inner surface of a transparent pane and the component is a sensor.

Abstract: An emergency oxygen system for an internal cabin of a vehicle includes a housing including one or more compartments, and one or more oxygen assemblies. The one or more oxygen assemblies include a mask and a fluid conduit. The mask is configured to be stowed within and deployed from the one or more compartments. A door is moveably secured to the housing. The door is moveable between a closed position underneath the one or more compartments to secure the mask within the one or more compartments, and an open position during a deployment event in which the mask drops down and laterally out of the one or more compartments. The mask is configured to hang down from a portion of the door when the door is in the open position. The emergency oxygen assemblies can be separate and distinct from passenger service units (PSUs).

Abstract: A resuscitation system for use in the resuscitation of a subject includes a computing device configured to input subject information and use the subject information to create a subject resuscitation strategy, a sensor configured to detect subject biosignals and use the subject biosignals to monitor for a subject cardiac arrest event, and a defibrillator configured to determine and deliver a subject resuscitation treatment. On determination of a subject cardiac arrest event, the sensor outputs sensor subject biosignals to the computing device. The computing device uses the sensor subject biosignals to adapt the subject resuscitation strategy and output the subject resuscitation strategy to the defibrillator. The defibrillator measures subject biodata and uses the subject biodata and the subject resuscitation strategy to determine and deliver a subject resuscitation treatment.

Abstract: A method for cleaning a load lock in a substrate processing system includes, in a first period, opening a first valve in fluid communication with a gas source to supply gas through a first vent into a gas volume of the load lock. The gas is supplied at a pressure and flow rate sufficient to disturb particles from surfaces of the load lock. The method includes, in a second period subsequent to the first period and with the first valve opened, opening a second valve in fluid communication with a pump and turning on the pump to flush the gas and particles from the gas volume of the load lock, and, in a third period subsequent to the second period, closing the first valve while continuing to pump the gas and the particles from the gas volume of the load lock via the second valve.

Abstract: Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.

Abstract: A mounting system configured to support a component relative to a mounting surface of a vehicle includes a mounting bracket including a mounting portion configured to be coupled to the mounting surface of the vehicle. The mounting bracket defining a component receptacle configured to support the component. The mounting bracket comprises a first plastic material. The mounting system also includes a seal coupled to the mounting bracket and configured to engage the mounting surface of the vehicle to inhibit ingress of debris between the mounting portion and the mounting surface of the vehicle. The seal comprises a second plastic material, different than the first plastic material. The mounting system may be included in a window assembly where the mounting surface of the vehicle is an inner surface of a transparent pane and the component is a sensor.

Abstract: A Reconfigurable, Flexible Multi-User (RFMU) electronically steered antenna (ESA) includes a top panel and a number of side panels that enable many contacts simultaneously with varying gain requirements from a single, in situ, installation. The top panel includes multiple subarrays and can communicate with flyover satellites, and the side panels can communicate with satellites flying past a side of the ESA. The top panel and the side panels can achieve a beam steering that covers a full or partial, variable gain, hemispheric field-of-view (FoV). The RFMU ESA terminal top and side panels are scalable using flexible modular building blocks. This enables increased contacts, increased gain or a combination thereof sized to meet desired performance.

Abstract: According to the disclosure there is provided an apparatus that assesses chest recoil of a subject during cardio pulmonary resuscitation (CPR) carried out by a rescuer on the subject and provides feedback to the rescuer. This apparatus may include a bio-signal measurement system configured to measure bio-signals of the subject. The apparatus may identify when CPR is required and may provide a CPR start signal to indicate when CPR should be started and this apparatus may identify when CPR is to be ceased. A CPR stop signal may be provided to a rescuer to indicate when CPR should be stopped. The apparatus may include electronic circuits capable of measuring impedance signals of the subject, may include sensors that sense bio-signals, and may include defibrillator shock electronics capable of providing a shock to the subject to restart or reset the heart of the subject.

Abstract: During operation, a system may obtain first and second magnetic resonance (MR) measurements associated with a biological lifeform, where the first and second MR measurements were acquired while the biological lifeform engaged in free breathing and/or without prospective gating based on a cardiac rhythm of the biological lifeform, and the first and second MR measurements were acquired at different times. Then, the system may dynamically determine first and second instances of one or more anatomical structures associated with the biological lifeform based at least in part on the first and second MR measurements, and a segmentation technique. Next, the system may perform a quantitative comparison of the first instance of the one or more anatomical structures and the second instance of the one or more anatomical structures, where the quantitative comparison has an uncertainty of less than a predefined value.

Abstract: Provided herein are machine learning-based methods for assessing the combined impact of multiple genetic variants, as well as the uses of such methods for various applications, such as in synthetic biology, personalized medicine, agricultural breeding, and genetic engineering. Also provided herein are exemplar computer-readable storage media and electronic devices for performing such methods.

Abstract: Use of CRISPR/Cas12d systems in eukaryotic organisms including plants and eukaryotic cells for genome engineering, and compositions used in such methods are disclosed.

Abstract: Methods for obtaining plant cells, plants, and plant parts, including soybean plant cells, plants, and plant parts, comprising synthetic polynucleotides that provide for increased expression of encoded RNA-guided endonucleases (RGEs), RNA guided nickase (RGNs), and RNA guided DNA binding proteins are disclosed. Also provided are soybean plant cells, plants, and plant parts comprising synthetic polynucleotides that provide for increased expression of encoded RNA-guided endonucleases (RGEs), RNA guided nickase (RGNs), and RNA guided DNA binding proteins.

Abstract: Provided herein is a method of delivering a biological material, including a genome-editing agent, to the interior of a plant cell wherein the method includes contacting the plant cell with a complex comprising the biological material and a fluorous agent. Also provided herein are compositions and reagents for practicing the method.

Abstract: The present disclosure relates to base-editing systems including a fusion protein including a DNA-binding domain and a cytidine deaminase domain and a non-protein uracil-DNA glycosylase inhibitor, and methods of using the same. The DNA-binding domains of base-editing systems of the present disclosure include domains with a variety of target region possibilities, which increase the number and type of sequences that can be edited. The npUGIs of the base-editing systems of the present disclosure improve UDG inhibition (e.g., UDG inhibition is more complete) and are suitable for use in a wide range of organisms.

Abstract: A method for cleaning a load lock in a substrate processing system includes, in a first period, opening a first valve in fluid communication with a gas source to supply gas through a first vent into a gas volume of the load lock. The gas is supplied at a pressure and flow rate sufficient to disturb particles from surfaces of the load lock. The method includes, in a second period subsequent to the first period and with the first valve opened, opening a second valve in fluid communication with a pump and turning on the pump to flush the gas and particles from the gas volume of the load lock, and, in a third period subsequent to the second period, closing the first valve while continuing to pump the gas and the particles from the gas volume of the load lock via the second valve.