Abstract: Provided herein are devices, systems, and methods for measuring the presence of an analyte of interest in a sample. Certain embodiments of the present disclosure are related to culture measurement systems that include a culture vial and a sensor, wherein the sensor is a pH sensor, a responsive label, or an indicator compound, such that the sensor is incorporated into the culture vial for measurement of the pH of the sample.

Abstract: Various embodiments of the present disclosure describe systems and methods for testing samples (e.g., biological samples, environmental samples, food samples etc.) for microbial contamination. For example, some embodiments describe an adapter assembly with a means to penetrate a septum of a collection vessel and permit gaseous communication between a headspace of the collection vessel and a sensor. In some embodiments, the gases in the headspace of the collection vessel can exit the collection vessel without contaminating the environment outside the system or allowing sample contamination. In some embodiments, the adapter assembly includes a membrane configured to prevent liquid in the collection vessel from contacting the sensor. In some embodiments, the adapter assembly can be used to access media inside the collection vessel for subculturing or aliquotting for another diagnostic process such as molecular diagnostics.

Abstract: Embodiments disclosed herein relate to methods and systems for performing an automated assay, and particularly to performing an assay on a plurality of samples on an automated instrument.

Abstract: Embodiments disclosed herein relate to methods and systems for performing an automated assay, and particularly to performing an assay on a plurality of samples on an automated instrument.

Abstract: Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Abstract: Various embodiments of the present disclosure describe systems and methods for testing samples (e.g., biological samples, environmental samples, food samples etc.) for microbial contamination. For example, some embodiments describe an adapter assembly with a means to penetrate a septum of a collection vessel and permit gaseous communication between a headspace of the collection vessel and a sensor. In some embodiments, the gases in the headspace of the collection vessel can exit the collection vessel without contaminating the environment outside the system or allowing sample contamination. In some embodiments, the adapter assembly includes a membrane configured to prevent liquid in the collection vessel from contacting the sensor. In some embodiments, the adapter assembly can be used to access media inside the collection vessel for subculturing or aliquotting for another diagnostic process such as molecular diagnostics.

Abstract: Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Abstract: Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Abstract: Embodiments disclosed herein relate to methods and systems for analysis of melting temperatures, and particularly to analysis of duplex nucleic acids.

Abstract: A process is disclosed for generating at least one partially double-stranded target nucleic acid, which contains at least one single-stranded region at a terminal end. The process comprises the steps of (a) providing at least one primer, P1, containing at least one labile nucleotide; (b) combining at least one target nucleic acid sequence with P1 to generate a double-stranded polynucleotide containing at least one labile nucleotide; (c) exposing the double-stranded polynucleotide to conditions that promote single-strand cleavage of the polynucleotide at the site of the at least one labile nucleotide of primer P1; and (d) exposing the cleaved polynucleotide to conditions that promote the dissociation of the cleaved portions of primer P1 from a terminal end. The labile nucleotide may be dUTP, wherein the single-stranded cleavage of the polynucleotide at the site of the labile nucleotide occurs by treatment with uracil N-glycosylase.

Abstract: A process is disclosed for generating at least one partially double-stranded target nucleic acid, which contains at least one single-stranded region at a terminal end. The process comprises the steps of (a) providing at least one primer, P1, containing at least one labile nucleotide; (b) combining at least one target nucleic acid sequence with P1 to generate a double-stranded polynucleotide containing at least one labile nucleotide; (c) exposing the double-stranded polynucleotide to conditions that promote single-strand cleavage of the polynucleotide at the site of the at least one labile nucleotide of primer P1; and (d) exposing the cleaved polynucleotide to conditions that promote the dissociation of the cleaved portions of primer P1 from a terminal end. The labile nucleotide may be dUTP, wherein the single-stranded cleavage of the polynucleotide at the site of the labile nucleotide occurs by treatment with uracil N-glycosylase.

Abstract: A fluorescently labeled nucleic acid having a hairpin structure between the fluorophore label and a point of attachment to a solid phase is useful as a probe to detect nucleic acid from a sample. The solid phase quenches the fluorophore label when the hairpin structure exists but this quenching is relieved by duplex formation between probe and a sample oligonucleotide. Probes for specific nucleic acid sequences can be immobilized as arrays on solid phase surfaces for detection of multiple nucleic acid sequences simultaneously from electrophoresis gels and from aqueous solutions. These probes and methods for their use can be combined with known solid phases, particularly those used for plasmon surface detection and electron transfer detection of nucleic acid. The probes can be washed and reused, and have other advantageous features over known probe methods.